Enhancement of α-Mangostin Wound Healing Ability by Complexation with 2-Hydroxypropyl-β-Cyclodextrin in Hydrogel Formulation

Abstract

α-Mangostin (α-M), one of the active compounds in Garcinia mangostana peel, has been effectively used in wound healing. However, its poor solubility in aqueous solution causes low bioavailability for skin ulcers, hindering its application in wound healing. The aim of this study was to improve the solubility of α-M through complex formation with 2-hydroxypropyl-β-cyclodextrin (α-M/HP-β-CD CX) and to evaluate the healing activity of the complex. The α-M/HP-β-CD CX was incorporated in a sodium carboxymethylcellulose hydrogel (α-M/HP-β-CD CX HG), and the in vivo healing activity was examined in mice. Evaluation of α-M/HP-β-CD CX HG, including organoleptic evaluation, homogeneity, pH, spreadability, swelling ratio, consistency, scanning electron microscopy (SEM), and in vitro drug release, was carried out. The complex formation of α-M/HP-β-CD CX was confirmed by FTIR and PXRD analysis. The solubility of the α-M/HP-β-CD CX in water linearly increased about 11.7-fold compared to α-M alone, and by 3.5-fold compared to the α-M/HP-β-CD physical mixture (α-M/HP-β-CD CX PM). The α-M/HP-β-CD CX HG was homogenous, the pH was found to be in the neutral range, the spread area was 5 cm, and the consistency was stable until 14 days. SEM analysis showed that α-M/HP-β-CD CX HG surged due to the porous structure of the HG. In addition, in vitro release of α-M from α-M/HP-β-CD CX HG was considerably increased compared to α-M/HP-β-CD PM HG and α-M HG. Notably, in vivo evaluation in mice showed that α-M/HP-β-CD CX HG significantly accelerated the wound healing ability compared to other HGs. Thus, α-M/HP-β-CD CX HG has potential as a new formulation of α-M for wound healing therapy.

Antibiotic-Loaded Psyllium Husk Hemicellulose and Gelatin-Based Polymeric Films for Wound Dressing Application

“…Antibiotic-loaded modern wound dressings (hydrogels, polymeric films, wafers, etc.) have been introduced to circumvent the drawbacks associated with the semisolid preparations and dry dressings [ 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. These modern dressings can locally deliver antibiotics, absorb wound exudate, and provide a moist environment to enhance cell proliferation at the wound site [ 14 ].…”

Methods for Improving Alpha-Mangostin Solubility: A Review

“…Beta cyclodextrin has 2 derivatives of 2.6 dimethyl- -cyclodextrin and 2-hydroxypropyl- -cyclodextrin, which are commonly used to form inclusion complexes with alphamangostin. 2-hydroxypropyl- -cyclodextrin is able to bind with alpha mangostin to form complex bonds in hydrogel formulation [79]. The results showed 2.6 dimethyl- -cyclodextrin results in a more soluble complex than 2-hydroxypropyl- -cyclodextrin [42].…”

ALPHA-MANGOSTIN (Garcinia mangostana Linn.) AND ITS POTENTIAL APPLICATION IN MITIGATING CHRONIC WOUND HEALING

“…The wound area was measured after 7 and 14 days of treatment. Based on their observation, α-mangostin/HP-β-CD CX had a faster wound closure rate compared to control groups (Wathoni et al, 2020).…”