Enhancing acetone biosynthesis and acetone–butanol–ethanol fermentation performance by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae integrated with exogenous acetate addition

Luo, Hongzhen; Ge, Laibing; Zhang, Jingshu; Ding, Jian; Rui, Chen; Shi, Zhongping

doi:10.1016/j.biortech.2015.09.116

Cited by 55 publications

(27 citation statements)

References 25 publications

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“…Acetone might be one of the potential oxygenate fuels which could be used because of its similar latent heating value relative to gasoline. Acetone (as also known as dimethyl ketone) may originate not only from coal, natural gas or petroleum sources but also from microbial fermentation [21]. It has been claimed that using acetone as an additive in gasoline could reduce emissions and increase efficiency due to its low flash point and smaller carbon chain [22,23].…”

Section: Introductionmentioning

confidence: 99%

An Experimental Study on the Potential Usage of Acetone as an Oxygenate Additive in PFI SI Engines

Meng

Zeng

et al. 2016

Energies

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To face the challenges of fossil fuel shortage and stringent emission norms, there is growing interest in the potential usage of alternative fuels such as bio-ethanol and bio-butanol in internal combustion engines. More recently, Acetone-Butanol-Ethanol (ABE), the intermediate product of bio-butanol fermentation, has been gaining a lot of attention as an alternative fuel. The literature shows that the acetone in the ABE blends plays an important part in improving the combustion performance and emissions, owing to its higher volatility. Acetone and ethanol are the low-value byproducts during bio-butanol production, so using acetone and ethanol as fuel additives may have both economic and environmental benefits. This study focuses on the differences in combustion, performance and emission characteristics of a port-injection spark-ignition engine fueled with pure gasoline (G100), ethanol-containing gasoline (E10 and E30) and acetone-ethanol-gasoline blends (AE10 and AE30 at A:E volumetric ratio of 3:1). The tests were conducted at 1200 RPM, under gasoline maximum brake torque (MBT) at 3 bar and 5 bar brake mean effective pressure (BMEP). Performance and emission data were measured under various equivalence ratios. Based on the comparison of combustion phasing, brake thermal efficiency, brake specific fuel consumption and various emissions of different fuels, it was found that using acetone as an oxygenate additive with the default ECU calibration (for gasoline) maintained the thermal efficiency and showed lower unburned HC emissions.

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Section: Introductionmentioning

confidence: 99%

An Experimental Study on the Potential Usage of Acetone as an Oxygenate Additive in PFI SI Engines

Meng

Zeng

et al. 2016

Energies

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“…xylose and cellobiose instead of glucose, to overcome catabolite repression in ABE fermentation with Clostridium saccharoperbutylacetonicum N1-4 led to production of 16g/L butanol without catabolite repression (Noguchi et al, 2013). Co-culturing of Clostridium acetobutylicum ATCC 824 with Saccharomyces cerevisiae (secreting favorable amino acids) aided in production of 14.0g/L butanol due to favourable redox balance (Luo et al, 2016). Co-culturing of engineered Clostridium cellulovorans and Clostridium beijerinckii in fermentation using corn cobs as substrate resulted in production of 11.5g/L butanol (Wen et al, 2017).Co-culturing of Clostridium beijerinckii F6 and Sachharomyces cerevisiae resulted in production of 12.75g/L butanol (Wu et al, 2019) (Table 8)…”

Section: Use Of Organic Acidsmentioning

confidence: 99%

Recent Advances in Microbial Production of Butanol as a Biofuel

Goyal

Khanna

2019

Int J Appl Sci Biotechnol

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In lieu of rising crude oil prices, exhaustion of petroleum feed stocks and environmental challenges, only renewable fuels have the potential to match the energy requirements of the future. Among the various renewable fuels, butanol has recently gained a lot of attention because of its advantages over other biofuels. Its microbial production by clostridia through ABE fermentation is being explored for improved yield and cost effectiveness. Using lignocellulosic wastes successfully for butanol production through ABE fermentation is a major breakthrough to deal with the future energy crisis. Genetic engineering of microbes to increase the carbon and redox balance, cell recycling, media optimization, mathematical modelling and tolerance improvement strategies are being attempted to overcome the hurdles of high production cost, by products formation leading to low yield and product toxicity. Along with genetic engineering major research is cantered on heterologous host engineering for improved butanol production and tolerance. This review highlights the recent advances in improving yield and tolerance to butanol in both Clostridial and heterologous hosts from genetic engineering and fermentation methodology aspects. Int. J. Appl. Sci. Biotechnol. Vol 7(2): 130-152

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“…Routes to produce acetone from petroleum eventually eclipsed fermentative processes, and recent research on C. acetobutylicum has predominantly focused on the production of bio‐butanol as a drop‐in replacement for gasoline, in which context acetone is often seen as an undesired byproduct to be minimized (Jang et al, ). However, acetone remains a valuable product in its own right (Luo et al, ). As a commodity chemical, acetone prices in recent years have exceeded those for ethanol, although it remains less valuable than n‐butanol.…”

Section: Introductionmentioning

confidence: 99%

“…replacement for gasoline, in which context acetone is often seen as an undesired byproduct to be minimized (Jang et al, 2012). However, acetone remains a valuable product in its own right (Luo et al, 2016).…”

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confidence: 99%

A synthetic enzymatic pathway for extremely thermophilic acetone production based on the unexpectedly thermostable acetoacetate decarboxylase from Clostridium acetobutylicum

Zeldes

Straub

Otten

et al. 2018

Biotech & Bioengineering

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One potential advantage of an extremely thermophilic metabolic engineering host (T ≥ 70°C) is facilitated recovery of volatile chemicals from the vapor phase of an active fermenting culture. This process would reduce purification costs and concomitantly alleviate toxicity to the cells by continuously removing solvent fermentation products such as acetone or ethanol, a process we are calling "bio-reactive distillation." Although extremely thermophilic heterologous metabolic pathways can be inspired by existing mesophilic versions, they require thermostable homologs of the constituent enzymes if they are to be utilized in extremely thermophilic bacteria or archaea. Production of acetone from acetyl-CoA and acetate in the mesophilic bacterium Clostridium acetobutylicum utilizes three enzymes: thiolase, acetoacetyl-CoA: acetate CoA transferase (CtfAB), and acetoacetate decarboxylase (Adc). Previously reported biocatalytic pathways for acetone production were demonstrated only as high as 55°C. Here, we demonstrate a synthetic enzymatic pathway for acetone production that functions up to at least 70°C in vitro, made possible by the unusual thermostability of Adc from the mesophile C. acetobutylicum, and heteromultimeric acetoacetyl-CoA:acetate CoA-transferase (CtfAB) complexes from Thermosipho melanesiensis and Caldanaerobacter subterraneus, composed of a highly thermostable α-subunit and a thermally labile β-subunit. The three enzymes produce acetone in vitro at temperatures of at least 70°C, paving the way for bio-reactive distillation of acetone using a metabolically engineered extreme thermophile as a production host.

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Enhancing acetone biosynthesis and acetone–butanol–ethanol fermentation performance by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae integrated with exogenous acetate addition

Cited by 55 publications

References 25 publications

An Experimental Study on the Potential Usage of Acetone as an Oxygenate Additive in PFI SI Engines

An Experimental Study on the Potential Usage of Acetone as an Oxygenate Additive in PFI SI Engines

Recent Advances in Microbial Production of Butanol as a Biofuel

A synthetic enzymatic pathway for extremely thermophilic acetone production based on the unexpectedly thermostable acetoacetate decarboxylase from Clostridium acetobutylicum

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