Enhancing acid tolerance of Leuconostoc mesenteroides with glutathione

Kim, Ji Eun; Eom, Hyun-Ju; Kim, Yujin; Ahn, Ji-Eun; Kim, Jeong Hwan; Han, Nam Soo

doi:10.1007/s10529-011-0815-1

Cited by 29 publications

(13 citation statements)

References 15 publications

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“…paraplantarum/pentosus, shared similar (GTG) 5 profile and showed same adaptation patterns. Several mechanisms are involved in LAB stress tolerance: glutathione system (Kim et al 2012;Zhang and Li 2013), the so-called general stress response or acid tolerance response (ATR) (van de Guchte et al 2002), and the ability to detoxify reactive oxygen species, thanks to antioxidant enzymes (An et al 2011;Bruno-Bárcena et al 2010;Ramesh et al 2011). Although LAB are catalase negative, other enzymes, such as superoxide dismutase, peroxidases or NADH oxidases might be involved in detoxification.…”

Section: Discussionmentioning

confidence: 99%

Identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves

Fessard¹,

Bourdon

Payet

et al. 2016

Can. J. Microbiol.

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From 6 samples of tropically grown fruits and leaves, 10 lactic acid bacteria belonging Leuconostoc, Weissella, and Lactobacillus species were isolated and identified by 16S rRNA gene sequencing and (GTG)5 fingerprinting. Acidification kinetics determined from BHI broth cultures showed genus-related patterns. In particular, Weissella cibaria appeared to act as a potent acidifier. Tolerance of isolates to acid, oxidative, or salt stress was highly variable and strain dependent. Isolate S14 (Leuconostoc pseudomesenteroides) growth was not affected by the presence of 0.05% H2O2, while Lactobacillus spp. isolates (S17 and S29) were the most tolerant to pH 4.5. The growth of 4 isolates, S5 (Leuconostoc mesenteroides), S14 and S10 (Leuconostoc pseudomesenteroides), and S27 (W. cibaria), was not affected by 5% NaCl. Nutritional beneficial properties were examined through measurement of antioxidant activities of short-term fermented pineapple juice, such as LDL oxidation and polyphenol content, and through exopolysaccharide formation from sucrose. Two isolates, S14 and S27, increased the antioxidant capacity of pineapple juice. The robust capacity of W. cibaria and of Leuconostoc pseudomesenteroides for vegetable lactic fermentation aimed to ameliorate food nutritional and functional quality was highlighted.

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Section: Discussionmentioning

confidence: 99%

Identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves

Fessard¹,

Bourdon

Payet

et al. 2016

Can. J. Microbiol.

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“…cremoris [75] and Leu. mesenteroides [76] from varying degree of acid challenge. Regulation of potassium export channels by GSH is not yet proven in LAB and the mechanism for protection is suggested to be either sacrificial action of GSH which prevents rapid fall of intracellular pH or glutathionylation of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) enzyme which helps to sustain glycolysis at the required level.…”

Section: Role Of Glutathione In Food Grade Labmentioning

confidence: 99%

Current status and emerging role of glutathione in food grade lactic acid bacteria

et al. 2012

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Lactic acid bacteria (LAB) have taken centre stage in perspectives of modern fermented food industry and probiotic based therapeutics. These bacteria encounter various stress conditions during industrial processing or in the gastrointestinal environment. Such conditions are overcome by complex molecular assemblies capable of synthesizing and/or metabolizing molecules that play a specific role in stress adaptation. Thiols are important class of molecules which contribute towards stress management in cell. Glutathione, a low molecular weight thiol antioxidant distributed widely in eukaryotes and Gram negative organisms, is present sporadically in Gram positive bacteria. However, new insights on its occurrence and role in the latter group are coming to light. Some LAB and closely related Gram positive organisms are proposed to possess glutathione synthesis and/or utilization machinery. Also, supplementation of glutathione in food grade LAB is gaining attention for its role in stress protection and as a nutrient and sulfur source. Owing to the immense benefits of glutathione, its release by probiotic bacteria could also find important applications in health improvement. This review presents our current understanding about the status of glutathione and its role as an exogenously added molecule in food grade LAB and closely related organisms.

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“…is susceptible to acidic stress, low pH (≤5·0) resulted in a low growth rate and reduced PLA production (Kim et al . ). This indicated that the initial pH of the medium might be an important factor that affects PLA production.…”

Section: Resultsmentioning

confidence: 97%

Production of natural antimicrobial compound d -phenyllactic acid using Leuconostoc mesenteroides ATCC 8293 whole cells involving highly active d -lactate dehydrogenase

Shin

Lee

et al. 2014

Lett Appl Microbiol

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Significance and Impact of the Study: This is the first study on the production of D-phenyllactic acid, which is a natural antimicrobial compound, from phenylpyruvate using Leuconostoc mesenteroides cells. The strain, ATCC 8293, that was used in the study, possesses high stereoselectivity and delivers a high yield. Therefore, it might be a promising candidate for use in large-scale production facilities and in fermented foods. D-PLA, and the yields were between 75Á2 and 83Á3%. Higher conversion yields were obtained at pH 6Á0-7Á0 when growing cells were used, while the optimum pH range was broader for resting cells. The time required for the complete conversion of PPA into PLA could be shortened to 3 h using resting cells. D-ldh, an enzyme encoded by the LEUM_1756 gene of Leuc. mesenteroides ATCC 8293, was found to be responsible for the conversion of PPA into PLA. The K m and k cat values of the enzyme for PPA were found to be 15Á4 mmol l À1 and 5645 s À1 , respectively. The conditions required for the efficient production of D-PLA were optimized for both growing and resting cells of Leuc. mesenteroides, with special emphasis on achieving high stereoselectivity and conversion yield.

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Enhancing acid tolerance of Leuconostoc mesenteroides with glutathione

Cited by 29 publications

References 15 publications

Identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves

Identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves

Current status and emerging role of glutathione in food grade lactic acid bacteria

Production of natural antimicrobial compound d -phenyllactic acid using Leuconostoc mesenteroides ATCC 8293 whole cells involving highly active d -lactate dehydrogenase

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