Enhancing anti-cancer activity of erlotinib by antibody conjugated nanofibrin - In vitro studies on lung adenocarcinoma cell lines

Vedakumari, Sathyaraj Weslen; Senthil, Rethinam; Sathiya, S.; Babu, Chidambaram Saravana; Sastry, T. P.

doi:10.1016/j.matchemphys.2018.11.061

Cited by 15 publications

(8 citation statements)

References 43 publications

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“…Firstly, the cell cycle analysis in the present study indicates a decrease of G0/G1 and an increase of sub-G0/G1, confirming that apoptosis may result from cell cycle arrest. Vedakumari et al showed that BV-ERB-FN-treated A549 cells exhibited efficient cell cycle arrest with a decrease in the G0/G1 phase and an increase in the sub-G0/G1 phase resulting in apoptosis of cancer cells [55]. Secondly, our study showed that the expression level of caspase-3/7 was increased after the PAM treatment in the A549 cells.…”

Section: Discussionsupporting

confidence: 59%

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Joh

Chung

et al. 2020

Oxidative Medicine and Cellular Longevity

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Cold atmospheric plasma (CAP) has been reported to have strong anticancer effects in vitro and in vivo. CAP has been known to induce apoptosis in most cancer cells by treatment to cells using direct and indirect treatment methods. There are many reports of apoptosis pathways induced by CAP, but for indirect treatment, there is still a lack of fundamental research on how CAP can cause apoptosis in cancer cells. In this study, we applied an indirect treatment method to determine how CAP can induce cancer cell death. First, plasma-activated medium (PAM) was produced by a 2.45 GHz microwave-excited atmospheric pressure plasma jet (ME-APPJ). Next, the amounts of various reactive species in the PAM were estimated using colorimetric methods. The concentration of NO2– and H2O2 in PAM cultured with cancer cells was measured, and intracellular reactive oxidative stress (ROS) changes were observed using flow cytometry. When PAM was incubated with A549 lung cancer cells, there was little change in NO2– concentration, but the concentration of H2O2 gradually decreased after 30 min. While the intracellular ROS of A549 cells was rapidly increased at 2 hours, there was no significant change in that of PAM-treated normal cells. Furthermore, PAM had a significant cytotoxic effect on A549 cells but had little effect on normal cell viability. In addition, using flow cytometry, we confirmed that apoptosis of A549 cells occurred following flow cytometry and western blot analysis. These results suggest that among various reactive species produced by PAM, hydrogen peroxide plays a key role in inducing cancer cell apoptosis.

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Section: Discussionsupporting

confidence: 59%

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Joh

Chung

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…In addition to the amine groups of antibodies, [117] the carboxyl groups of antibodies can be activated for the conjugation with nanoparticles through the formation of amide bonds via the EDC/NHS chemistry. [118] Despite the convenience of utilizing the natural amine or carboxyl groups in antibodies, this direct EDC/NHS-based conjugation strategy still has its own limitations. One apparent problem of the EDC/NHS chemistry is that the direct attachment may cause the self-polymerization of antibodies since the amine and carboxyl groups are both present in the antibody molecules.…”

Section: -Ethyl-3-(3-dimethylaminopropyl)carbodiimide/n-hydroxysuccin...mentioning

confidence: 99%

Antibody‐Incorporated Nanomedicines for Cancer Therapy

Chen

2022

Advanced Materials

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“…Nanoparticles (core size of 198 nm and shell size of 210 nm) showed a BCZ conjugation efficiency of 82% and ζ potential close to +5 mV [ 70 ]. BCZ has been conjugated on the surface of erlotinib-loaded fibrin nanoparticles by reacting amino group of fibrin with carboxyl group of BCZ ( d = 79 nm; ζ potential = +17 mV; spherical shape with smooth surface) [ 71 ].…”

Section: Development Of Nanoparticulate Drug Delivery Systems For Bczmentioning

confidence: 99%

“…BCZ-loaded PLA nanoparticles decreased survival of A549 cells and human breast cancer cells (MCF-7 and MDA-MB-231) cells [ 50 ]. Also using A549 cells, BCZ conjugated in erlotinib-loaded fibrin nanoparticles demonstrated higher cytotoxicity (IC50 = 0.84 µM) and apoptosis (61.7 ± 1.3%) than free erlotinib (IC50 = 2.3 µM; 18.35 ± 2.1%); causing a gradual decrease in G0/G1 phase, an increase in sub-G0/G1 phase, and a marked intracellular uptake [ 71 ]. Similarly, BCZ and erlotinib co-loaded in lipid-polymer hybrid nanoparticles were more cytotoxic in comparison to the free drugs against two human non-small cell lung cancer cell lines (A549 and H1975) with marked cellular uptake (about 70%) in A549 cells [ 65 ].…”

Section: Pharmacological Applicationsmentioning

confidence: 99%

“…Therefore, these nanocarriers can decrease the multiple administrations by parenteral or intravitreal route, improving the patient compliance to treatment. Furthermore, nanoformulations with BCZ presented good tolerability [ 35 , 49 , 52 ] and hemocompatibility [ 38 , 54 , 65 , 71 ]. The intravitreal injection of nanoparticles with BCZ did not cause ocular toxicity in rabbits [ 44 ] neither in mice [ 45 ] or in rats [ 36 ].…”

Section: Limitations and Advantages Of Bcz Nanocarriersmentioning

confidence: 99%

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Organic Nanocarriers for Bevacizumab Delivery: An Overview of Development, Characterization and Applications

et al. 2021

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Bevacizumab (BCZ) is a recombinant humanized monoclonal antibody against the vascular endothelial growth factor, which is involved in the angiogenesis process. Pathologic angiogenesis is observed in several diseases including ophthalmic disorders and cancer. The multiple administrations of BCZ can cause adverse effects. In this way, the development of controlled release systems for BCZ delivery can promote the modification of drug pharmacokinetics and, consequently, decrease the dose, toxicity, and cost due to improved efficacy. This review highlights BCZ formulated in organic nanoparticles providing an overview of the physicochemical characterization and in vitro and in vivo biological evaluations. Moreover, the main advantages and limitations of the different approaches are discussed. Despite difficulties in working with antibodies, those nanocarriers provided advantages in BCZ protection against degradation guaranteeing bioactivity maintenance.

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Enhancing anti-cancer activity of erlotinib by antibody conjugated nanofibrin - In vitro studies on lung adenocarcinoma cell lines

Cited by 15 publications

References 43 publications

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Antibody‐Incorporated Nanomedicines for Cancer Therapy

Organic Nanocarriers for Bevacizumab Delivery: An Overview of Development, Characterization and Applications

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