Enhancing antimicrobial activity of TiO2/Ti by torularhodin bioinspired surface modification

Ungureanu, Camelia; Dumitriu, Cristina; Popescu, Simona; Enculescu, Monica; Tofan, Vlad; Popescu, Marian; Pîrvu, Cristian

doi:10.1016/j.bioelechem.2015.09.001

Cited by 61 publications

(42 citation statements)

References 63 publications

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“…Regarding xylose cultivation, the favored torularhodin and torulene production at higher C/N might surpass the low r lip and r x values obtained, by offering product diversification and possible profitability as these non-conventional carotenoids are not produced at a large scale and only a number of yeasts and fungi can synthesize them naturally (Kot et al 2018). Furthermore, studies show that they possess interesting properties against cancer (Du et al 2016), microbial activity (Ungureanu et al 2016), and as antioxidants (Dimitrova et al 2013). In terms of processes, results obtained with R. toruloides show a possibility to control the profile of carotenoids during cultivation throughout the change on C/N ratio and dilution rate.…”

Section: Discussionmentioning

confidence: 99%

C/N ratio and carbon source-dependent lipid production profiling in Rhodotorula toruloides

Lopes

Bonturi

Kerkhoven

et al. 2020

Appl Microbiol Biotechnol

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Microbial oils are lipids produced by oleaginous microorganisms, which can be used as a potential feedstock for oleochemical production. The oleaginous yeast Rhodotorula toruloides can co-produce microbial oils and high-value compounds from lowcost substrates, such as xylose and acetic acid (from hemicellulosic hydrolysates) and raw glycerol (a byproduct of biodiesel production). One step towards economic viability is identifying the best conditions for lipid production, primarily the most suitable carbon-to-nitrogen ratio (C/N). Here, we aimed to identify the best conditions and cultivation mode for lipid production by R. toruloides using various low-cost substrates and a range of C/N ratios (60, 80, 100, and 120). Turbidostat mode was used to achieve a steady state at the maximal specific growth rate and to avoid continuously changing environmental conditions (i.e., C/N ratio) that inherently occur in batch mode. Regardless of the carbon source, higher C/N ratios increased lipid yields (up to 60% on xylose at a C/N of 120) but decreased the specific growth rate. Growth on glycerol resulted in the highest specific growth and lipid production (0.085 g lipids/gDW*h) rates at C/Ns between 60 and 100. We went on to study lipid production using glycerol in both batch and fed-batch modes, which resulted in lower specific lipid production rates compared with turbisdostat, however, fed batch is superior in terms of biomass production and lipid titers. By combining the data we obtained in these experiments with a genome-scale metabolic model of R. toruloides, we identified targets for improvements in lipid production that could be carried out either by metabolic engineering or process optimization. Keywords Rhodotorula toruloides. Microbial oil. Biorefineries. Genome-scale metabolic model. Flux balance analysis. Carbon to nitrogen ratio. Alternative substrates Helberth Júnnior Santos Lopes and Nemailla Bonturi contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

C/N ratio and carbon source-dependent lipid production profiling in Rhodotorula toruloides

Lopes

Bonturi

Kerkhoven

et al. 2020

Appl Microbiol Biotechnol

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“…The flat band potential for uncoated Ti50Zr alloy estimated by Mott–Schottky equation is −0.86 V, with about 100 mV more negative than the values reported in literature for TiO 2 , −0.75 V (vs. SCE), but much more positive than the values observed for ZrO 2 , −1.58 V vs Ag/AgCl/KCl . Taking into account the ratio of 2.30 for TiO 2 :ZrO 2 , estimated from EDX analysis, assuming that only TiO 2 and ZrO 2 is on the surface, the E FB value is expected to be closer to that of TiO 2 .…”

Section: Resultsmentioning

confidence: 67%

Influence of electrospun TiO₂ nanowires on corrosion resistance and cell response of Ti50Zr alloy

Manole

Dinischiotu²,

Nica³

et al. 2018

Materials & Corrosion

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TiO2 nanowires are fabricated by electrospinning on Ti50Zr alloy, by changing the elaboration conditions. The surface covered with nanowires is characterized by performing scanning electronic microscopy and contact angle measurements. The best surface from the point of view of nanowires organization is chosen based on experimental conditions. The electrochemical stability is tested in Fusayama saliva. The response of HGF‐1 gingival fibroblasts cells is established and discussed. Cellular response is evaluated by the cell viability, lactate dehydrogenase test, nitric oxide level and interleukines IL‐6 and IL‐8 secretion. A very small increase of IL‐8 expression was noticed only for the samples obtained at 15 kV, whereas for IL‐6 no change compared to control was observed. This small increase could be related to the structural changes in the TiO2 semiconductor nanowires surface. Such data suggest that this alloy covered with TiO2 n‐type semiconductor nanowires can be successfully used in biomedical applications without generating significant inflammatory processes.

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“…In order to investigate potential biological properties of complexes 1 – 4 , the microbial activities of 1 – 4 were investigated. Gram‐negative bacteria is more prone to cytotoxic activity than that of the gram‐positive one due to the more complicated cell wall structure consisting of several peptidoglycan layers in gram‐positive bacteria . Hence, we selected E. coli (gram‐negative) bacterial strain to explain the antimicrobial effect complexes 1 – 4 .…”

Section: Resultsmentioning

confidence: 99%

Mononuclear metal (II) complexes of a Bis(organoamido)phosphate ligand with antimicrobial activities against Escherichia coli

Pal

Tarafdar

Sinha

et al. 2017

Applied Organom Chemis

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A new tripodal ligand [PO(NH 2 MePy) 3 ] (L) ( 2 MePy = 2-(4-methyl pyridyl)) have been synthesized by treating phosphorous oxychloride with 2-Amino-4methylpyridine in toluene under refluxing condition. The ligand was appeared as a white solid and characterized by several standard analytical and spectroscopic techniques such as FT-IR, NMR ( 1 H, 13 C{ 1 H} and 31 P{ 1 H}) and ESI-MS spectroscopy. The ligand (L) undergone metal-assisted hydrolysis of one P-N bond when treated it with hydrated metal nitrates, M(NO 3 ) 2 ·xH 2 O (M = Zn, Cu, Co and Ni) under hydrothermal reaction condition in DMF-H 2 O (1:1). This results in the formation of four mononuclear complexes [{PO 2 (NH 2 MePy) 2 } 2 M] [M = Zn (1), Cu (2), Co (3), Ni (4)], where ligand (L) hydrolyses to a anionic bis(organoamido)phosphate, [PO 2 (NH 2 MePy) 2 ] − . All complexes were completely characterized by various analytical techniques and their solid state molecular structures were established by single crystal X-ray diffraction. All complexes are isostructural with a metal (II) ion situating at the centre of a distorted octahedron. Two tridentate [PO 2 (NH 2 MePy) 2 ] − ligands are coordinated to metal(II) ion through N-and Odonor atoms, thus neutralizing the charge of the complex. Optical properties of all complexes in solid state have been studied. Moreover, antimicrobial activities of complexes 1-4 have been explored. To the best of our knowledge, this is the first report of such compounds investigated for their antimicrobial activities. KEYWORDS antimicrobial activity, bis(organoamido)phosphate ligand, mononuclear metal complex, P-N bond hydrolysis, X-ray diffraction

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Enhancing antimicrobial activity of TiO2/Ti by torularhodin bioinspired surface modification

Cited by 61 publications

References 63 publications

C/N ratio and carbon source-dependent lipid production profiling in Rhodotorula toruloides

C/N ratio and carbon source-dependent lipid production profiling in Rhodotorula toruloides

Influence of electrospun TiO₂ nanowires on corrosion resistance and cell response of Ti50Zr alloy

Mononuclear metal (II) complexes of a Bis(organoamido)phosphate ligand with antimicrobial activities against Escherichia coli

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Enhancing antimicrobial activity of TiO2/Ti by torularhodin bioinspired surface modification

Cited by 61 publications

References 63 publications

C/N ratio and carbon source-dependent lipid production profiling in Rhodotorula toruloides

C/N ratio and carbon source-dependent lipid production profiling in Rhodotorula toruloides

Influence of electrospun TiO2 nanowires on corrosion resistance and cell response of Ti50Zr alloy

Mononuclear metal (II) complexes of a Bis(organoamido)phosphate ligand with antimicrobial activities against Escherichia coli

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Influence of electrospun TiO₂ nanowires on corrosion resistance and cell response of Ti50Zr alloy