Enhancing beta-carotene content in potato by rnai-mediated silencing of the beta-carotene hydroxylase gene

Eck, Joyce Van; Conlin, Brian J.; Garvin, David F.; Mason, Hugh S.; Navarre, Duroy A.; Brown, Charles R.

doi:10.1007/bf02986245

Cited by 111 publications

(41 citation statements)

References 32 publications

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“…Thus, it is possible that, by silencing of the CHY1, CHY2, LUT5, and LCY-e genes, the levels of b-carotene in golden tubers may increase even further. This has been experimentally confirmed for the LCY-e and CHY genes (Diretto et al, 2006(Diretto et al, , 2007bvan Eck et al, 2007).…”

Section: Callimentioning

confidence: 51%

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Transcriptional-Metabolic Networks in β-Carotene-Enriched Potato Tubers: The Long and Winding Road to the Golden Phenotype

Diretto¹,

Al‐Babili²,

Tavazza³

et al. 2010

Plant Physiology

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Vitamin A deficiency is a public health problem in a large number of countries. Biofortification of major staple crops (wheat [Triticum aestivum], rice [Oryza sativa], maize [Zea mays], and potato [Solanum tuberosum]) with b-carotene has the potential to alleviate this nutritional problem. Previously, we engineered transgenic "Golden" potato tubers overexpressing three bacterial genes for b-carotene synthesis (CrtB, CrtI, and CrtY, encoding phytoene synthase, phytoene desaturase, and lycopene b-cyclase, respectively) and accumulating the highest amount of b-carotene in the four aforementioned crops. Here, we report the systematic quantitation of carotenoid metabolites and transcripts in 24 lines carrying six different transgene combinations under the control of the 35S and Patatin (Pat) promoters. Low levels of B-I expression are sufficient for interfering with leaf carotenogenesis, but not for b-carotene accumulation in tubers and calli, which requires high expression levels of all three genes under the control of the Pat promoter. Tubers expressing the B-I transgenes show large perturbations in the transcription of endogenous carotenoid genes, with only minor changes in carotenoid content, while the opposite phenotype (low levels of transcriptional perturbation and high carotenoid levels) is observed in Golden (Y-B-I) tubers. We used hierarchical clustering and pairwise correlation analysis, together with a new method for network correlation analysis, developed for this purpose, to assess the perturbations in transcript and metabolite levels in transgenic leaves and tubers. Through a "guilt-by-profiling" approach, we identified several endogenous genes for carotenoid biosynthesis likely to play a key regulatory role in Golden tubers, which are candidates for manipulations aimed at the further optimization of tuber carotenoid content.

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Section: Callimentioning

confidence: 51%

“…The first one was a "blocking" strategy and relied on the posttranscriptional gene silencing of key biosynthetic steps: the CHY hydroxylase, acting on b-carotene (Diretto et al, 2007b;van Eck et al, 2007), the ZEP epoxidase, acting on zeaxanthin , and the LCY-e cyclase, acting on lycopene and starting the a-branch (Diretto et al, 2006; Fig. 1).…”

mentioning

confidence: 99%

Transcriptional-Metabolic Networks in β-Carotene-Enriched Potato Tubers: The Long and Winding Road to the Golden Phenotype

Diretto¹,

Al‐Babili²,

Tavazza³

et al. 2010

Plant Physiology

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“…White fleshed potatoes have 50-100 μg per 100 g fresh weight (FW) of total carotenoids while yellow-fleshed potatoes have 100-350 μg per 100 g FW. Dark-yellow potatoes may contain up to 1,000 μg per 100 g FW while the highest recorded contents reach to 2,600 μg per 100 g FW (Brown et al 1993;Lu et al 2001;Nesterenko and Sink 2003;Brown 2005;Brown et al 2005;Reyes et al 2005Brown et al 2006Brown et al 2007;Van Eck et al 2007;Brown 2008a, b).…”

Section: Introductionmentioning

confidence: 99%

Variability of Phytonutrient Content of Potato in Relation to Growing Location and Cooking Method

et al. 2008

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“…Most potatoes are a poor source of beta-carotene, although some lines produce high levels of zeaxanthin, a xanthophyll that is derived from beta-carotene by the activity of the enzyme beta-carotene hydroxylase (BCH; Brown et al 1993). By silencing the BCH gene in potato using RNAi, Van Eck et al (2007) were able to significantly increase beta-carotene content of tubers, even in lines that normally accumulate only low levels of zeaxanthin. Although the beta-carotene levels reached in these lines were lower than carotenoid-rich vegetables such as carrots or sweet potatoes, biofortification of potatoes offers an opportunity to provide added nutritional benefits to a food source that is already popular globally.…”

Section: Nutritionmentioning

confidence: 99%

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

et al. 2015

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Potato is the world's most important vegetable crop, with nearly 400 million tons produced worldwide every year, lending to stability in food supply and socioeconomic impact. In general, potato is an intensively managed crop, requiring irrigation, fertilization, and frequent pesticide applications in order to obtain the highest yields possible. Important traits are easy to find in wild relatives of potato, but their introduction using traditional breeding can take 15-20 years. This is due to sexual incompatibility between some wild and cultivated species, a desire to remove undesirable wild species traits from adapted germplasm, and difficulty in identifying broadly applicable molecular markers. Fortunately, potato is amenable to propagation via tissue culture and it is relatively easy to introduce new traits using currently available biotech transformation techniques. For these reasons, potato is arguably the crop that can benefit most by modern biotechnology. The benefits of biotech potato, such as limited gene flow to conventionally grown crops and weedy relatives, the opportunity for significant productivity and nutritional quality gains, and reductions in production cost and environmental impact, have the potential to influence the marketability of newly developed varieties. In this review we will discuss current and past efforts to develop biotech potato varieties, traits that could be impacted, and the potential effects that biotech potato could have on the industry.Resumen La papa es el cultivo hortícola más importante en el mundo, con cerca de 400 millones de toneladas producidas a nivel mundial anualmente, acreditando la estabilidad en el suministro de alimentos e impacto socioeconómico. En general, la papa es un cultivo manejado intensivamente, que requiere riego, fertilización y aplicaciones frecuentes de plaguicidas para obtener los más altos rendimientos posibles. Los caracteres importantes son fáciles de encontrar en parientes silvestres de la papa, pero su introducción usando el mejoramiento tradicional puede llevar de 15 a 20 años. Esto es debido a la incompatibilidad sexual entre algunas especies silvestres y cultivadas, el deseo para eliminar características indeseables de las especies silvestres del germoplasma adaptado, y la dificultad en la identificación de marcadores moleculares aplicables ampliamente. Afortunadamente, la papa es receptiva a la propagación por cultivo de tejidos y es relativamente fácil la introducción de nuevos caracteres usando técnicas biotecnológicas de transformación actualmente disponibles. Por estas razones, la papa es probablemente el cultivo que se puede beneficiar mayormente por la biotecnología moderna. Los beneficios de la papa biotecnológica, como el flujo genético limitado a cultivos que se siembran convencionalmente y a los parientes como malezas, la oportunidad para productividad significativa y logros en calidad nutricional, y las reducciones en los costos de producción e impacto ambiental, tienen el potencial para influenciar la comercialización...

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Enhancing beta-carotene content in potato by rnai-mediated silencing of the beta-carotene hydroxylase gene

Cited by 111 publications

References 32 publications

Transcriptional-Metabolic Networks in β-Carotene-Enriched Potato Tubers: The Long and Winding Road to the Golden Phenotype

Transcriptional-Metabolic Networks in β-Carotene-Enriched Potato Tubers: The Long and Winding Road to the Golden Phenotype

Variability of Phytonutrient Content of Potato in Relation to Growing Location and Cooking Method

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

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