Enhancing identification of cancer types via lowly-expressed microRNAs

Rasnic, Roni; Linial, Michal

doi:10.1093/nar/gkx210

Cited by 14 publications

(7 citation statements)

References 43 publications

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“…The emergence of de novo miRNA families has increased by more than three-fold in mammals, and these are overrepresented within introns, likely catalyzed by host gene transcription (Meunier et al, 2013). These miRNAs may be equally as, if not more, important in human-specific diseases, as evidenced by a recent study indicating that profiles of lowly expressed miRNAs significantly enhance cancer phenotyping (Rasnic et al, 2017). Moreover, the effects of most miRNAs on protein expression are very modest (Baek et al, 2008), and the majority of miRNA deletion models do not exhibit any observable defects (Miska et al, 2007).…”

Section: Biogenesis and Function Of Mirnasmentioning

confidence: 99%

The role of microRNAs in chronic respiratory disease: recent insights

Stolzenburg¹,

Harris²

2017

Biological Chemistry

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Chronic respiratory diseases encompass a group of diverse conditions affecting the airways, which all impair lung function over time. They include cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD) and asthma, which together affect hundreds of millions of people worldwide. MicroRNAs (miRNAs), a class of small non-coding RNAs involved in post-transcriptional gene repression, are now recognized as major regulators in the development and progression of chronic lung disease. Alterations in miRNA abundance occur in lung tissue, inflammatory cells, and freely circulating in blood and are thought to function both as drivers and modifiers of disease. Their importance in lung pathology has prompted the development of miRNA-based therapies and biomarker tools. Here, we review the current literature on miRNA expression and function in chronic respiratory disease and highlight further research that is needed to propel miRNA treatments for lung disorders towards the clinic.

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Section: Biogenesis and Function Of Mirnasmentioning

confidence: 99%

The role of microRNAs in chronic respiratory disease: recent insights

Stolzenburg¹,

Harris²

2017

Biological Chemistry

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“…Therefore, it is necessary to further explore the pathogenesis of AB at the molecular level. miRNAs are small non-coding RNAs that are 19-24 nucleotides in length [15]. They can mediate gene expression by binding the 3'-untranslated region of target messenger RNAs (mRNAs), leading to translational repression or degradation of the target mRNAs [16,17].…”

Section: Introductionmentioning

confidence: 99%

Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

et al. 2020

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Background:Ameloblastoma (AB) is a common odontogenic epithelial tumor, with locally invasive behavior and high recurrence. In this study, we hypothesized that miR-524-5p could be involved in the tumor microenvironment by targeting interleukin-33 (IL-33)/suppression of tumorigenicity 2 (ST2) in AB. Material/Methods:The microRNA (miRNA) expression profile of AB tissues and normal oral mucosa tissues (NOM; 6 paired samples) was analyzed. The miRNAs with fold change ³2 and P<0.05 were considered to be differentially expressed. Among them, downregulated miR-524-5p was verified by real-time qPCR. Potential targets of miR-524-5p were predicted by bioinformatics analysis. The expression levels of target genes were detected using real-time qPCR and Western blot, respectively. Immunohistochemistry analysis of target genes was performed, and we also assessed the correlation between miR-524-5p and its target. Results:Microarray analysis results first indicated miR-524-5p is a downregulated miRNA in AB tissues. Real-time qPCR results confirmed the expression pattern of miR-524-5p in AB tissues. Moreover, IL-33 and its receptor ST2 were significantly overexpressed. As shown in immunohistochemistry results, IL-33 was positively expressed in lymphocytes and plasma cells, suggesting that IL-33/ST2 participates in tumor immune responses in the tumor microenvironment. Correlation analysis suggested that miR-524-5p expression was negatively correlated with IL-33/ST2. Conclusions:Our findings reveal that downregulated miR-524-5p can participate in the tumor microenvironment of AB by targeting the IL-33/ST2 axis.

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“…The seed region of miRNAs can form a complementary base pair with the 3'-untranslated region (3'UTR) of target gene mRNAs, leading to mRNA degradation or translation inhibition (5). The dysregulation of miRNAs has been reported to be associated with the initiation and development of a number of cancer types (6). In colorectal cancer, a number of miRNAs, including miR-17-5p and miR-21, have been revealed to be key drivers of tumorigenesis (7,8).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑3651 promotes colorectal cancer cell proliferation through directly repressing T‑box transcription factor 1

et al. 2020

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colorectal cancer is a commonly diagnosed gastrointestinal malignancy worldwide with a high mortality rate. Accumulating evidence has indicated that the expression of a number of microRNAs (miRNAs) is associated with the development of colorectal cancer. However, the precise molecular mechanism of these miRNAs in regulating cancer progression is yet to be determined. In the present study, miR-3651 was demonstrated to be overexpressed in colorectal cancer tissues compared with normal tissues, and to be associated with the tumor-node-metastasis stage. The downregulation of miR-3651 was found to induce growth arrest and apoptosis in colorectal cancer cells. In addition, western blot analysis demonstrated that the downregulation of miR-3651 inactivated PI3K/AKT and MAPK/ERK signaling in colorectal cancer cells. Bioinformatics analysis predicted T-box transcription factor 1 (TBX1) as a potential target gene of miR-3651, and a dual-luciferase reporter assay confirmed that TBX1 was directly repressed by miR-3651. The results of the current study also indicated that TBX1 was associated with the miR-3651 mediated activation of oncogenic signaling and colorectal cancer cell proliferation. In conclusion, the results of the current study revealed the oncogenic potential of miR-3651 in colorectal cancer.

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Enhancing identification of cancer types via lowly-expressed microRNAs

Cited by 14 publications

References 43 publications

The role of microRNAs in chronic respiratory disease: recent insights

The role of microRNAs in chronic respiratory disease: recent insights

Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

MicroRNA‑3651 promotes colorectal cancer cell proliferation through directly repressing T‑box transcription factor 1

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