MicroRNA‑3651 promotes colorectal cancer cell proliferation through directly repressing T‑box transcription factor 1

Chang-feng, LI; Ding, Dayong; Gao, Yongjian; Li, Yongchao

doi:10.3892/ijmm.2020.4458

Cited by 8 publications

(12 citation statements)

References 41 publications

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“…Western blot in this study also showed that decreased expression of this marker leads to inactivation of PI3K / AKT and MAPK / ERK signaling pathways in colorectal cancer cells. Finally, this study suggested that this marker has oncogenic potential in colorectal cancer (33).…”

Section: Discussionmentioning

confidence: 54%

“…It is located in the nucleus of a human cell on chromosome 9 (9q22.31) and is estimated to be 24 nucleotides in length. Changes in the expression of this miRNA have been studied in various cancers, including esophageal cancer (25), nasopharynx (29), liver cells (32) and colorectal (30,33). Also, according to articles that examined such biomarkers in precancerous lesions compared to cancerous and normal tissue, and observed changes in the incidence of these biomarkers (31), resulting in the possibility of changes in the expression of this miRNA in cancer.…”

Section: Discussionmentioning

confidence: 99%

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miRNA-3651: a Diagnostic Marker in Oral Squamous Cell Carcinoma?

Saeidmohamadi

Ghorbanpour

Jolehar

2021

Preprint

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Background and aim: microRNAs are a group of small non-coding single-stranded RNAs that control post-transcriptional gene expression. They can act as oncogenes or tumor suppressors by amplifying or preventing the expression of certain genes. present study was conducted to assess the expression of miRNA-3651 in paraffin blocks of oral squamous cell carcinoma (OSCC) cells using qRT-PCR method in Islamic Azad university, dental branch of Tehran in 1399.Material and methods: This case-control study was conducted on 20 paraffin blocks of irritation fibroma (IF) as control group and 20 paraffin blocks of patients with oral squamous cell carcinoma as case group. After RNA extraction, qRT-PCR was performed. all experiments were repeated 3 times for each sample. Eventually the data were analyzed by SPSS 24 statistics software. Differences in miRNA expression levels between the two groups were compared using the independent samples t-test. In order to evaluate the correlation between mean levels of miRNA-3651 marker and different variables (grade, age and sex of patients), Kruskal-Wallis test, Pearson's correlation coefficient test and independent samples t-test were used, respectively.Results: The results showed that the mean expression of this biomarker method was 10.15± 5.44 rpm in normal tissue and 8.11± 1.57 rpm in cancerous tissue. Despite the lower expression of miRNA-3651 in cancerous tissue samples than normal samples, this decrease was not statistically significant (P> 0.05). There was no significant difference between the mean level of miRNA-3651 marker and different grades, age and sex of patients (p> 0.05).Conclusion: In the end, it seems that the evaluation of changes in the expression of miRNAs such as miRNA-3651, can be a minimally invasive method, in early detection and screening of patients with OSCC. Decreased expression of miRNA-3651 marker in cancerous tissue compared to normal tissue indicates the importance of these biomarkers, including miRNA-3651 in the diagnosis of oral cancer, and the researchers of this project suggest further and broader investigations on the mechanism of action and signaling cascades associated with this marker in oral cancer.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

miRNA-3651: a Diagnostic Marker in Oral Squamous Cell Carcinoma?

Saeidmohamadi

Ghorbanpour

Jolehar

2021

Preprint

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“…Previous studies have demonstrated microRNA–T-box member regulatory modulation in several biological contexts in both homeostasis and diseases, in particular for Tbx1 , Tbx3 , and Tbx5 . Tbx1 is targeted by miR-3651 in colorectal cancer, promoting cell proliferation [ 62 ]; by miR-451a in cutaneous basal carcinoma, suppressing cell growth [ 63 ]; and by miR-96 in dental epithelial progenitor cells [ 64 ]. In addition, indirect evidence supports a role of the miR-17-92 cluster in the regulation of Tbx1 expression during midface development [ 68 ] and for miR-182 during otocyst-derived cell differentiation [ 88 ].…”

Section: Discussionmentioning

confidence: 99%

“…Among those T-box genes expressed during cardiogenesis, microRNA-mediated post-transcriptional regulation has only been reported for Tbx1 , Tbx3 , and Tbx5 in distinct biological contexts. Tbx1 is targeted by miR-3651 in colorectal cancer, promoting cell proliferation [ 62 ]; by miR-451a in cutaneous basal carcinoma, suppressing cell growth [ 63 ]; and by miR-96 in dental epithelial progenitor cells [ 64 ]. Furthermore, only indirect evidence supports a role of the miR-17-92 cluster in regulation of Tbx1 expression during myocardial differentiation from cardiac progenitors [ 65 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Spatio-Temporal Regulation of T-Box Gene Expression by microRNAs during Cardiac Development

Alzein

Lozano-Velasco

Hernández-Torres

et al. 2021

JCDD

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Cardiovascular development is a complex process that starts with the formation of symmetrically located precardiac mesodermal precursors soon after gastrulation and is completed with the formation of a four-chambered heart with distinct inlet and outlet connections. Multiple transcriptional inputs are required to provide adequate regional identity to the forming atrial and ventricular chambers as well as their flanking regions; i.e., inflow tract, atrioventricular canal, and outflow tract. In this context, regional chamber identity is widely governed by regional activation of distinct T-box family members. Over the last decade, novel layers of gene regulatory mechanisms have been discovered with the identification of non-coding RNAs. microRNAs represent the most well-studied subcategory among short non-coding RNAs. In this study, we sought to investigate the functional role of distinct microRNAs that are predicted to target T-box family members. Our data demonstrated a highly dynamic expression of distinct microRNAs and T-box family members during cardiogenesis, revealing a relatively large subset of complementary and similar microRNA–mRNA expression profiles. Over-expression analyses demonstrated that a given microRNA can distinctly regulate the same T-box family member in distinct cardiac regions and within distinct temporal frameworks, supporting the notion of indirect regulatory mechanisms, and dual luciferase assays on Tbx2, Tbx3 and Tbx5 3′ UTR further supported this notion. Overall, our data demonstrated a highly dynamic microRNA and T-box family members expression during cardiogenesis and supported the notion that such microRNAs indirectly regulate the T-box family members in a tissue- and time-dependent manner.

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“…MicroRNAs (miRs) are small noncoding RNAs that can target the 3ʹ untranslated region (3ʹUTR) of message RNAs (mRNAs) to degrade mRNAs and/or inhibit mRNA translation. Until now, several studies have reported the roles of miRs in cell proliferation, migration, invasion, and angiogenesis of CC [19][20][21]. Our previous study demonstrated that miR-6727-5p was highly expressed in CC tissues and promoted the proliferation of CC cells [22].…”

Section: Introductionmentioning

confidence: 99%