Enhancing oxidation activity and stability of iso-1-cytochrome c and chloroperoxidase by immobilization in nanostructured supports

Oxidative transformation of dibenzothiophene by chloroperoxidase enzyme immobilized on (1D)-γ-Al2O3 nanorods

“…2, where physical adsorption of enzyme in both supports exhibited a sharp initial rise, suggesting a high affinity between CPO and Al-nR-500 • C/700 • C/900 • C materials; this may be caused by favorable electrostatic interactions between enzyme and support. Indeed, this phenomenon has been recognized as a key role in the immobilization process [1,2], where the physical adsorption of enzyme and its stability are controlled by electrostatic interactions [32].…”

“…Nanocatalysis encompasses chemical and biological catalysis, and bring together advances in nanotechnology and bionanotechnology such as the immobilization of biocatalyst on nanostructured materials commonly used as supports in heterogeneous catalysis [1,2]. Enzymes are biocatalysts and play key roles in several metabolic reactions, they functioning with a great specificity to catalyze chemical reactions, showing advantageous applications in industry and refinery due to their chemo and stereospecificity in mild conditions [3].…”

“…However, due to their proteic origin, they are highly labile to several physicochemical factors such as changes in temperature and pH, exposure to organic solvents and high concentrations of salts [4]. To avoid the unfolding and reduction of activity, enzymes have been entrapped by immobilization inside the voids of nanostructured materials like carbon nanotubes [5], nanospheres [6], nanogold particles [7], flower-like nanocrystals [8], MCM-41 [1], SBA-15 [1,9], SBA-16 [10] and mesocellular foam [1,4], among others. Mesoporous materials (2-50 nm) exhibited structural properties at the nanometer scale; the large surface area is one of the remarkable, because this allows the material to fulfill technological demands such as adsorption, separation, catalysis, sensors and immobilization of huge biomolecules like enzymes and drugs [11].…”

“…In a previous work, some of us reported the immobilization of CPO from Caldariomyces fumago and yeast iso-1-cytochrome c (CYC-Sc) from Saccharomyces cerevisiae in some nanostructured supports i.e. MCM-41, SBA-15 and MCF, resulting in considerable improvements on the catalytic enzymatic activity and protein stability of referred enzymes [1]. In this work, we report the use of novel (1D)-␥-Al 2 O 3 nanorods linked networks (Al-nR), which have been recently synthesized [24], for CPO enzyme immobilization and their application for oxidative transformation processes.…”

Bienzymatic nanoreactors composed of chloroperoxidase–glucose oxidase on Au@Fe3O4 nanoparticles: Dependence of catalytic performance on the bioarchitecture

“…Furthermore, the stability, under both storage and operational conditions, e.g., towards denaturation by heat or organic solvents, can be enhanced [7]. Currently, a range of supports have been reported for the immobilization of CPO, such as mesoporous supports [6,8,9], solgels [10,11], multi-walled carbon nanotubes [12,13] and other inorganic or organic solid supports [14,15]. The binding of an enzyme to a support (carrier) can be physical, ionic, or covalent in nature.…”