Enhancing PPARγ by HDAC inhibition reduces foam cell formation and atherosclerosis in ApoE deficient mice

Strategies to gain novel Alzheimer’s disease diagnostics and therapeutics using modulators of ABCA transporters

“… 403 Decreased intracellular cholesterol levels were also observed. 402 Trichostatin A reduced aortic atherosclerotic plaques in high-fat diet-fed Apoe knock-out mice, 410 and an upregulation of ABCA1, PPAR-γ, and LXR-α/β protein levels was observed in aortic cells as well as peritoneal macrophages. 410 …”

“…Several natural compounds target the PPAR pathway, such as the flavonoids homoeriodictyol, 402 hesperetin-7- O -β- d -glucopyranoside, 402 scutellarein, 403 and the antimycotic trichostatin A. 410 These compounds increased Abca1 402 and Pparg 402 mRNA as well as ABCA1, 402 , 410 PPAR-α, 403 and PPAR-γ 402 , 410 protein levels in vitro 402 , 410 and in vivo . 403 Decreased intracellular cholesterol levels were also observed.…”

Echinococcus granulosus cyst fluid suppresses inflammatory responses by inhibiting TRAF6 signalling in macrophages

“…Collected peritoneal exudate cells were washed with phosphatebuffered saline (PBS) three times and centrifuged at 1500 rpm for 5 min. Cells were resuspended and plated in six-well plates at a density of 1 × 10 6 cells per well in DMEM containing L-glutamine supplemented with 10% fetal bovine serum (FBS) according to previous reports (Chen et al, 2020;Gao et al, 2020;He et al, 2020). After overnight culture, the non-adherent cells were removed, and the attached cells (enriched in peritoneal macrophages) were treated with LPS (1 μg mL −1 ) or LPS and EgCF (2.15 mg mL −1 ) for the indicated periods as a previous report (Hou et al, 2020).…”

Histone Deacetylases (HDACs) and Atherosclerosis: A Mechanistic and Pharmacological Review

“…Additionally, PPARγ is a transcriptional factor critically involved in lipid metabolism and possesses anti-inflammation properties (Vallée et al, 2019). Recent study showed that, by activating PPARγ through elevating acetylation of C/EBPα (CCAAT enhancer binding protein α), TSA increased ABCA1/ABCG1 expression and reduced TNF-α/IL-1β, which contributed to the inhibition of foam cell formation and atherogenesis (Gao et al, 2020).…”

“…In ox-LDL-treated HAECs and aortas from ApoE −/− mice, HDAC6 was upregulated, and its selective inhibitor tubacin prevented endothelial dysfunction and the development of AS (Leucker et al, 2017). Several HDACi such as TSA (Gao et al, 2020), metacept-1 (Vinh et al, 2008), BuA (He and Moreau, 2019), and SAHA (Ye et al, 2018;Manea et al, 2020) have been demonstrated to inhibit the development of AS.…”