The analysis of the loss of distributed photovoltaic power generation systems involves the interests of energy users, energy-saving service companies, and power grid companies, so it has always been the focus of the industry and society in some manner or another. However, the related analysis for an actual case that considers different cooperative corporations' benefits is lacking in the presently available literature. This paper takes the distributed rooftop photovoltaic power generation project in an industrial park as the object, studies the analysis and calculation methods of line loss and transformer loss, analyzes the change of transformer loss under different temperatures and different load rates, and compares the data and trend of electricity consumption and power generation in industrial parks before and after the photovoltaic operation. This paper explores and practices the analysis method of the operating loss of distributed photovoltaic power generation and provides an essential reference for the benefit analysis and investment cost estimation of distributed photovoltaic power generation systems in industrial parks. The analyzed results reveal that the change loss is stable after the photovoltaic is connected, and there is no additional transformer loss. And before and after the photovoltaic system installation, there was no significant change in the total monthly data difference between the total meter and the sub-meter.