Enhancing the in vitro and in vivo detection of aneuploidy by fluorescence in situ hybridization with the use of bromodeoxyuridine as a proliferation marker

Balakrishnan, Sharada; Payawal, J; Schuler, Maik; Hasegawa, L.; Eastmond, David A.

doi:10.1016/s1383-5718(02)00220-6

Cited by 13 publications

(6 citation statements)

References 29 publications

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“…Numeric chromosome imbalance (aneuploidy) is a hallmark of most solid tumours, whether spontaneous or induced by carcinogens (Pathak & Multani 2006). Data on chromosomal alterations in chemically induced urinary bladder carcinomas are scarce, with only a few studies having been reported (Debiec-Rychter et al 1989;Balakrishnan et al 2002;Vecchione et al 2004). Here, we describe the DNA content in low-grade and highgrade papillary carcinomas and the preliminary cytogenetic analysis in one low-grade papillary carcinoma and in one high-grade papillary carcinoma of the rat urinary bladder.…”

Section: Discussionmentioning

confidence: 93%

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DNA study of bladder papillary tumours chemically induced by N‐butyl‐N‐(4‐hydroxybutyl) nitrosamine in Fisher rats

Oliveira

Adega

Palmeira

et al. 2006

Int J Experimental Path

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To examine DNA abnormalities in bladder papillary tumours induced by N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) in female rats, using image cytometric DNA analysis and cytogenetics. Thirty female rats were exposed to BBN in their drinking water for 20 weeks. One group of 10 animals served as controls. The animals exposed to BBN were killed at a rate of two per week, with the bladder being collected under aseptic conditions and those tumours with exophytic growth removed. The nuclear DNA content of the tumours was evaluated using image cytometric analysis. In two rats part of the tumour pieces was stipulated for culturing. Cytogenetic analysis was performed on at least 30 cells from each cell population and on both tumours. Papillary carcinomas were classified as low grade and high grade. DNA ploidy studies were carried out on 28 low-grade and 21 high-grade papillary carcinomas. Histograms obtained by image analysis showed that a normal urothelium was diploid; 28.6% and 100% of low-and high-grade papillary carcinomas were aneuploid respectively. Both tumours used for cell culture showed multiple numerical and structural chromosome alterations and several marker chromosomes. Image cytometric DNA analysis proved to be a good and reliable method for examining DNA alterations in papillary bladder carcinomas. The present findings establish that the DNA content is statistically different between low-grade and high-grade papillary carcinomas and that deviation from the diploid number is markedly higher in the high-grade ones. In addition, the occurrence of marker chromosomes seems to be related to the aggressiveness of the tumour.

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Section: Discussionmentioning

confidence: 93%

“…Data on chromosomal alterations in chemically induced urinary bladder carcinomas are scarce, with only a few studies having been reported (Debiec‐Rychter et al. 1989; Balakrishnan et al. 2002; Vecchione et al.…”

mentioning

confidence: 99%

DNA study of bladder papillary tumours chemically induced by N‐butyl‐N‐(4‐hydroxybutyl) nitrosamine in Fisher rats

Oliveira

Adega

Palmeira

et al. 2006

Int J Experimental Path

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“…There are only scarce and indirect evidence of the possibility of the action of aneugens in dividing cells. For example, Balakrishnan et al [11] demonstrated that in vitro effects of noscapine and vincristine on rat lymphocytes and in vivo effects of vinblastine on hepatocytes caused an increase in the hyperploidy rate depending on the agent concentration only in dividing cells, incorporating bromodeoxyuridine, whereas this effect was absent in quiescent cells.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells of humans exposed to adverse environmental factors

Nazarenko¹,

Timoshevsky²

2005

Russ J Genet

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Fluorescence in situ hybridization (FISH) was used to compare aneuploidy rates in four autosomes and two sex chromosomes in interphase nuclei of noncultivated (quiescent) and cultivated (induced to divide with phytohemagglutinin (PHA)) leukocytes in people engaged in nuclear-chemical industry and in a control group of people not exposed to mutagenic factors occupationally or at home. The overall rates of numerical chromosome aberrations for all of the six chromosomes studied showed little difference, although a higher rate of loss of the X-and Y-chromosomes was observed in the exposed group. In individuals exposed to several adverse environmental factors, the overall rate of numerical chromosome aberrations in cultivated cells after at least one DNA replication cycle exceeded that in noncultivated cells by 52% ( P = 0.01), whereas only a trend for its increase was observed in the control group (23%, P = 0.25). Thus, the effect of adverse environmental factors in humans caused more than a twofold increase in the difference between the rates of aneuploid cells in cultivated and noncultivated leukocytes in the exposed group as compared to control. It is conjectured that cell division is accompanied by the expression of potential damage of mitotic chromatid segregation apparatus accumulated in vivo. These defects, realized during cell division, bring about numerical chromosome aberrations.

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“…Moreover, the researcher should not overlook another significant fact: the aneugen effect is manifested only in proliferating cells. An examination of the effect of preparations of plant origin, antitussive (noskapin) and antitumorous (vincristine and vinblastin), on rat lymphocytes and hepatocytes in vitro and in vivo showed that the hyperploidy frequency increased only in dividing cells with incorporated bromodeoxyuridine, while this effect was absent in G 0 cells [43].…”

Section: Agents Inducing Aneuploidy In Cells Of Rodents and Humansmentioning

confidence: 99%

Interphase cytogenetics in estimation of genomic mutations in somatic cells

2005

Russ J Genet

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Enhancing the in vitro and in vivo detection of aneuploidy by fluorescence in situ hybridization with the use of bromodeoxyuridine as a proliferation marker

Cited by 13 publications

References 29 publications

DNA study of bladder papillary tumours chemically induced by N‐butyl‐N‐(4‐hydroxybutyl) nitrosamine in Fisher rats

DNA study of bladder papillary tumours chemically induced by N‐butyl‐N‐(4‐hydroxybutyl) nitrosamine in Fisher rats

Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells of humans exposed to adverse environmental factors

Interphase cytogenetics in estimation of genomic mutations in somatic cells

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