Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions

Joshi, Rohit; Sahoo, Khirod Kumar; Singh, Anil Kumar; Anwar, Khalid; Pundir, Preeti; Gautam, Raj Kumar; Krishnamurthy, S. L.; Sopory, Sudhir K.; Pareek, Ashwani; Singla‐Pareek, Sneh L.

doi:10.1093/jxb/erz462

Cited by 98 publications

(58 citation statements)

References 85 publications

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“…However, the transgenic lines in this study do not appear to have progressed to commercial production. Improved tolerance to drought, salinity and sodic conditions (soil with an exchangeable sodium of >6% of the cation exchange capacity) was also shown using an ABA-inducible promoter to target the transgene [25]. In both studies, effects could have arisen through ROS scavenging and protection of cell membranes by trehalose.…”

Section: Ricementioning

confidence: 99%

“…• Importance of T6P signalling in crops: For the improvement of crop yields there are likely to be few individual genes that can be targeted, yet for T6P signalling there are examples of several TPS and TPP genes that underlie yield traits [13,25,43,45]. Further confirmation of T6P as a major regulator of yield was shown where increasing T6P chemically in wheat improved grain size and growth recovery from drought [42].…”

Section: Perspectivesmentioning

confidence: 99%

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Trehalose 6-phosphate signalling and impact on crop yield

Paul

Watson

Griffiths

2020

Biochemical Society Transactions

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The domestication and breeding of crops has been a major achievement for mankind enabling the development of stable societies and civilisation. Crops have become more productive per unit area of cultivated land over the course of domestication supporting a current global population of 7.8 billion. Food security crops such as wheat and maize have seen large changes compared with early progenitors. Amongst processes that have been altered in these crops, is the allocation of carbon resources to support larger grain yield (grain number and size). In wheat, reduction in stem height has enabled diversion of resources from stems to ears. This has freed up carbon to support greater grain yield. Green revolution genes responsible for reductions in stem height are known, but a unifying mechanism for the active regulation of carbon resource allocation towards and within sinks has however been lacking. The trehalose 6-phosphate (T6P) signalling system has emerged as a mechanism of resource allocation and has been implicated in several crop traits including assimilate partitioning and improvement of yield in different environments. Understanding the mode of action of T6P through the SnRK1 protein kinase regulatory system is providing a basis for a unifying mechanism controlling whole-plant resource allocation and source-sink interactions in crops. Latest results show it is likely that the T6P/SnRK1 pathway can be harnessed for further improvements such as grain number and grain filling traits and abiotic stress resilience through targeted gene editing, breeding and chemical approaches.

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Section: Ricementioning

confidence: 99%

Section: Perspectivesmentioning

confidence: 99%

Trehalose 6-phosphate signalling and impact on crop yield

Paul

Watson

Griffiths

2020

Biochemical Society Transactions

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“…The production of ROS singlet oxygen ( 1 O 2 ) under excessive light inhibits D1 protein synthesis and hampers the repair of photodamaged PSII, dampened carbohydrate replenishment and senescence. Similarly, under heat stress, electrons from NADH produced by the soluble, membrane-bound complexes at the inner mitochondrial membrane are disrupted or uncoupled [ 139 , 140 ]. During stress acclimation, plants undergo metabolite changes that involve reactive oxygen intermediates (ROI) production and defense regulated genes during stress, such as ROI scavenging enzymes and heat shock proteins [ 141 ].…”

Section: Impact Of Individual Combined and Sequential Stresses On Plantsmentioning

confidence: 99%

Elucidating the Response of Crop Plants towards Individual, Combined and Sequentially Occurring Abiotic Stresses

Anwar

Joshi

Dhankher

et al. 2021

IJMS

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In nature, plants are exposed to an ever-changing environment with increasing frequencies of multiple abiotic stresses. These abiotic stresses act either in combination or sequentially, thereby driving vegetation dynamics and limiting plant growth and productivity worldwide. Plants’ responses against these combined and sequential stresses clearly differ from that triggered by an individual stress. Until now, experimental studies were mainly focused on plant responses to individual stress, but have overlooked the complex stress response generated in plants against combined or sequential abiotic stresses, as well as their interaction with each other. However, recent studies have demonstrated that the combined and sequential abiotic stresses overlap with respect to the central nodes of their interacting signaling pathways, and their impact cannot be modelled by swimming in an individual extreme event. Taken together, deciphering the regulatory networks operative between various abiotic stresses in agronomically important crops will contribute towards designing strategies for the development of plants with tolerance to multiple stress combinations. This review provides a brief overview of the recent developments in the interactive effects of combined and sequentially occurring stresses on crop plants. We believe that this study may improve our understanding of the molecular and physiological mechanisms in untangling the combined stress tolerance in plants, and may also provide a promising venue for agronomists, physiologists, as well as molecular biologists.

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“…One example is rice cultivar R-64 which, through genetic modification and overexpression of trehalose-synthesizing trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes and fusion constructs trehalose-6-phosphate synthase/phosphatase (TPSP) from Escherichia coli, could tolerate multiple stresses like drought and salinity. The transgenic rice plants showed higher chlorophyll content, low sodium accumulation, high grain yield, and high trehalose accumulation (Joshi et al, 2020). Trehalose is a non-toxic carbon molecule characterized by its ability to accumulate at high concentrations in the cytoplasm and maintain turgidity besides its ability to evoke plant defense mechanisms (Schluepmann et al, 2004).…”

Section: Trehalose In Plants For Abiotic Stress Tolerancementioning

confidence: 99%

Deciphering the Role of Trehalose in Tripartite Symbiosis Among Rhizobia, Arbuscular Mycorrhizal Fungi, and Legumes for Enhancing Abiotic Stress Tolerance in Crop Plants

et al. 2020

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Drought is a critical factor limiting the productivity of legumes worldwide. Legumes can enter into a unique tripartite symbiotic relationship with root-nodulating bacteria of genera Rhizobium, Bradyrhizobium, or Sinorhizobium and colonization by arbuscular mycorrhizal fungi (AMF). Rhizobial symbiosis provides nitrogen necessary for growth. AMF symbiosis enhances uptake of diffusion-limited nutrients such as P, Zn, Cu, etc., and also water from the soil via plant-associated fungal hyphae. Rhizobial and AMF symbioses can act synergistically in promoting plant growth and fitness, resulting in overall yield benefits under drought stress. One of the approaches that rhizobia use to survive under stress is the accumulation of compatible solutes, or osmolytes, such as trehalose. Trehalose is a non-reducing disaccharide and an osmolyte reported to accumulate in a range of organisms. High accumulation of trehalose in bacteroids during nodulation protects cells and proteins from osmotic shock, desiccation, and heat under drought stress. Manipulation of trehalose cell concentrations has been directly correlated with stress response in plants and other organisms, including AMF. However, the role of this compound in the tripartite symbiotic relationship is not fully explored. This review describes the biological importance and the role of trehalose in the tripartite symbiosis between plants, rhizobia, and AMF. In particular, we review the physiological functions and the molecular investigations of trehalose carried out using omics-based approaches. This review will pave the way for future studies investigating possible metabolic engineering of this biomolecule for enhancing abiotic stress tolerance in plants.

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Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions

Abstract: Marker-free transgenic lines of rice are developed with enhanced trehalose accumulation that is associated with improved grain yield under salinity, sodicity, and drought stresses.

Cited by 98 publications

References 85 publications

Trehalose 6-phosphate signalling and impact on crop yield

Trehalose 6-phosphate signalling and impact on crop yield

Elucidating the Response of Crop Plants towards Individual, Combined and Sequentially Occurring Abiotic Stresses

Deciphering the Role of Trehalose in Tripartite Symbiosis Among Rhizobia, Arbuscular Mycorrhizal Fungi, and Legumes for Enhancing Abiotic Stress Tolerance in Crop Plants

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