Enhancing β-mannanase production by controlling fungal morphology in the bioreactor with microparticle addition

Mannooligosaccharide production by β‐mannanase enzyme application from coffee extract

“…However, before each β-mannanase fermentation, the cultures were re-cultivated and the fresh spores grown were employed for inoculation. For long-term storage, the cultures were kept at −80°C in 20% glycerol (Germec, Yatmaz, Karahalil, & Turhan, 2017;Karahalil, Germec, Karaoglan, et al, 2019;Karahalil, Germec, & Turhan, 2019;Ozturk, Cekmecelioglu, & Ogel, 2010;Yatmaz, Germec, Karahalil, & Turhan, 2020;Yatmaz, Karahalil, Germec, Ilgin, & Turhan, 2016;Yatmaz, Karahalil, Germec, Oziyci, et al, 2016). For inoculation, the salt-tween solution (0.8% NaCl (150 ml) and 0.005% Tween-80 (150 ml)) were individually prepared and sterilized at 121.1°C for 15 min.…”

“…β-Mannanase fermentation process was performed at 204 rpm, 5.0 pH, 0.5 vvm aeration rate, and 30°C by the inoculation of the prepared spore solution (300 ml, 1%, v/v) for 10 days in a 30-L stirred-tank bioreactor (Sartorius Stedim Biostad, C-Plus, Gottingen, Germany) (submitted to a journal). At the end of the fermentation, β-mannanase activity and specific β-mannanase activity of the fermented broth were measured as 180 U/ml and 1,176.47 U/mg, respectively, by standard analysis conditions in our previous studies (Germec et al, 2017;Karahalil, Germec, Karaoglan, et al, 2019;Karahalil, Germec, et al, 2019;Yatmaz et al, 2020;Yatmaz, Karahalil, Germec, Ilgin, et al, 2016;Yatmaz, Karahalil, Germec, Oziyci, et al, 2016). One unit of β-mannanase activity is equal to the amount of enzyme that generates 1 µmole of D-mannose at 50°C per minute.…”

The effects of mannanase activity on viscosity in different gums

“…The activity of β-mannanase was determined using a spectrophotometer (Thermo Scientific Evolution 201 UV-Vis, Manassas, MA, USA) at 540 nm as described in our previous studies (Germec et al, 2017;Karahalil, Germec, Karaoglan, et al, 2019;Karahalil, Germeç, et al, 2019;Yatmaz et al, 2020;Yatmaz, Karahalil, Germec, Ilgin, et al, 2016;Yatmaz, Karahalil, Germec, Oziyci, et al, 2016). One unit of enzyme activity (1 U = 16.7 nanokatal) is equal to the amount of enzyme that manufactures 1 µmole of D-mannose at 50°C per minute (Bailey, Biely, & Poutanen, 1992;Ozturk et al, 2010).…”

“…Nevertheless, Aspergillus sp. are also utilized in a widespread manner for the generation of microbial enzymes such as inulinase , 2020a, 2020bIlgın, Germec, & Turhan, 2020), phytase (Coban, Demirci, & Turhan, 2015a, 2015b, protease (Kalaskar, Kasinathan, Subrahmanyam, & Rao, 2020), lipase (Mandari, Nema, & Devarai, 2020), polygalacturonase (Karahalil et al, 2017), amylase (Pasin et al, 2020), cellulase (Dong et al, 2019), xylanase (de Souza et al, 2020 aside from β-mannanases (Duruksu, Ozturk, Biely, Bakir, & Ogel, 2009;Germec, Yatmaz, Karahalil, & Turhan, 2017;Karahalil, Germec, Karaoglan, et al, 2019;Karahalil, Germeç, & Turhan, 2019;Ozturk, Cekmecelioglu, & Ogel, 2010;Yatmaz, Germec, Karahalil, & Turhan, 2020;Yatmaz, Karahalil, Germec, Ilgin, & Turhan, 2016;Yatmaz, Karahalil, Germec, Oziyci, et al, 2016).…”

Scale‐up processing with different microparticle agent for β‐mannanase production in a large‐scale stirred tank bioreactor

“…In order to produce β-mannanase at low cost, many economical materials and industrial wastes were evaluated as substrate, including sugar beet molasses (Ozturk, Cekmecelioglu, & Ogel, 2010), whey (Karahalil, Germeç, et al, 2019), and coffee industry waste (Favaro et al, 2020). In this study, carob pod extract (CPE), which was evaluated for numerous fermentation processes such as ethanol, lactic acid, inulinase, and β-mannanase (Germec, Cheng, Karhan, Demirci, & Turhan, 2019;Germec, Karhan, Bialka, Demirci, & Turhan, 2018;Germec et al, 2020;Germec, Turhan, Demirci, & Karhan, 2016;Germec, Turhan, Karhan, & Demirci, 2015Germec, Yatmaz, Karahalil, & Turhan, 2017;Ilgın, Germec, & Turhan, 2019;Turhan, Bialka, Demirci, & Karhan, 2010;Yatmaz, Germec, Karahalil, & Turhan, 2020;Yatmaz, Karahalil, Germec, Ilgin, & Turhan, 2016;Yatmaz, Karahalil, Germec, Oziyci, et al, 2016), was used as an alternative carbon source in the production of β-mannanase.…”