Enrichment and Purification of Polyphenol Extract from Sphallerocarpus gracilis Stems and Leaves and in Vitro Evaluation of DNA Damage-Protective Activity and Inhibitory Effects of α-Amylase and α-Glucosidase

Abstract

An efficient preparative separation method for Sphallerocarpus gracilis stems and leaves polyphenols (SGslP) was established in this study. An X-5 macroporous adsorption resin was selected for the purification of the SGslP, and the polyphenol content of the purified SGslP (PSGslP) was increased 5.11-fold from 8.29% to 42.38% after one treatment run. The chemical composition of the PSGslP was analyzed by HPLC-MS/MS, and the predominant compounds were found to be luteolin-7-glucoside, acacetin-7-acetyglycoside and its isomers. In addition, the PSGslP was evaluated in vitro to determine the DNA damage-protective activity and inhibitory effects of α-amylase and α-glucosidase. The results indicated that the PSGslP exhibited significant protective activities against both ROO‚ and ‚OH radical-induced DNA damage. Moreover, the PSGslP exerted a dose-dependent inhibition effect on α-glucosidase but no inhibitory effect on α-amylase. These findings indicate that the Sphallerocarpus gracilis stems and leaves are good natural sources of antioxidants and are potent inhibitors of α-glucosidase activity and are potential anti-diabetic inhibitor.

Phenolic composition, antioxidant capacity and inhibitory effects on α-glucosidase and lipase of immature faba bean seeds

“…Acarbose, a α-glucosidase inhibitor, was used as positive control in many types of research to evaluate the inhibitory effect of bioactive compounds on α-glucosidase. [3,30] Surprisingly, in the present study, the IC 50 values of the inhibitory effects of the phenolic extracts were even significant lower than that of the acarbose. Additionally, the inhibitory activities on α-glucosidase found in this study were markedly higher than those of reported for other beans.…”

“…The α-glucosidase inhibitory activity was measured as described by Ma et al [30] with slight modifications. Briefly, 0.1 mL of pehnolic solutions or acarbose solutions with different concentrations were added to 0.1 mL of 1.25 U/mL α-glucosidase solution.…”

Nutritional properties and biological activities of kiwifruit (Actinidia) and kiwifruit products under simulated gastrointestinal in vitro digestion

“…Foods, especially fruits rich in polyphenols, offer an attractive means of managing postprandial hyperglycemia for type 2 diabetes by controlling starch breakdown and intestinal glucose absorption (30, 39). Thus, the inhibitory effects of α-amylase and α-glucosidase in kiwifruit and kiwifruit products during in vitro GI digestion were investigated (Fig.…”

“…The methods used for the α-amylase enzymatic inhibition assays performed in this study were adapted from those developed in a previous study (30), with slight modifications. In addition, the α-glucosidase inhibitory activity was measured as described in a previous report (30), with slight modifications. The inhibitory activity (I) was calculated using the following equation:…”

Influence of Sodium Alginate on Hypoglycemic Activity of Metformin Hydrochloride in the Microspheres Obtained by the Spray Drying

“…where IA is the percent of -amylase inhibition, C is the concentration of starch in the control reaction, and S is the concentration of starch in the test sample [34][35][36]. Differences between groups were considered to be significant at < 0.05.…”