Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro

Wan, Peng-Fei; Sheng, Zunlai; Han, Qiang; Yun-feng, Zhao; Cheng, Guangdong; Li, Yanhua

doi:10.1016/j.jchromb.2013.11.033

Cited by 66 publications

(48 citation statements)

References 37 publications

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“…Compared with other techniques, resin adsorption seemed to be the most suitable method due to its low cost, high efficiency and simple procedure (Sun et al, 2013). Nowadays, the technique has been widely employed in separating bioactive components from crude extracts of herbal raw materials, such as alkaloids from Aconiti kusnezoffii radix extracts (Liu et al,4 2014), phlorotannins from brown seaweed Ecklonia cava extracts (Kim et al, 2014), and flavonoids from Flos populi extracts (Wan, Sheng, Han, Zhao, Cheng, & Li, 2014). However, to the best of our knowledge, there is no report about the enrichment of total anthocyanins from N. tangutorun Bobr.…”

mentioning

confidence: 99%

In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits

Ding

et al. 2016

Food Chemistry

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mentioning

confidence: 99%

In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits

Ding

et al. 2016

Food Chemistry

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“…This result indicates that hydrogen bonding may play a significant role in the adsorption process of the XAD-7HP resin. According to Wan et al, at higher pH values, the phenolic hydroxyl groups in the polyphenols are dissociated, which resulting in the reduction of hydrogen bonding interactions and therefore, leading to lower adsorption capacity of polyphenols (Wan et al, 2014). The same result was reported by Wang et al , who found that pH 2.0 was optimal for the adsorption sample solution.…”

Section: Static Adsorption and Desorption On Xad-7hpmentioning

confidence: 61%

Optimation of Enrichment and Purification of Polyphenols From Blueberries (Vaccinium Spp.) by Macroporous Resins Xad-7hp

Jiao

Zhang

et al. 2017

Emir J Food Agric

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Introduction: Blueberries are rich sources of phenolic compounds and exhibit strong antioxidant activities and a wide variety of biological activities. Methods: Polyphenols were extracted from blueberry by solvent extraction method using ethanol solution. The purification of polyphenols from blueberries (Vaccinium spp.) by macroporous resin XAD-7HP was optimized by adsorption and desorption tests. The cellular antioxidant activities of the purified polyphenols were investigated. Results: The adsorption data indicated that the process was best explained by the Langmuir isotherm model. The best conditions were determined as: blueberry polyphenols concentration, 2.00 mg/g; pH 2.0; ethanol desorption solution concentration, 70 % (v/v); flow rates for feeding and elution, 1 and 2 bed volume (BV)/h, respectively; and a water wash volume of 3 BV. Under these conditions, the purity of the blueberry polyphenols was improved from 46.3 to 86.48 %. The EC50 (concentration for 50 % of maximal effect) and CAA (cellular antioxidant activity) values of the blueberry polyphenols were 151.58 mg/mL and 6.39 µmol QE /100 g sample, respectively.Conclusion: The results demonstrate that purification of polyphenols by macroporous resins XAD-7HP will provide foundation for the development of large-scale separation of blueberry polyphenols in industry.

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“…Generally, an ultrasonic method is used for the extraction of chlorogenic acid (Li et al, 2005;Mazvimba et al, 2012). Many materials, for example polyamide, macroporous resins (Wan et al, 2014;Zhang et al, 2008), silica gel column are used in separating and purifying chlorogenic acid. In recent years, polyamide has been widely used in separating plant effective components such as phenolic acids, flavonoids, quinones (Guo et al, 2011;Sun et al, 2013) and good results are obtained.…”

Section: Abbreviations: Ca Chlorogenic Acid; Lc-ms Liquid Chromatogmentioning

confidence: 99%

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

Zhao¹,

Chen²,

Li³

et al. 2015

Afr. J. Biotechnol.

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Methods for separation of chlorogenic acid (CA) from tobacco by-products were established. The liquid chromatography tandem mass spectrometry (LC-MS) method for identification and analysis of chlorogenic acid from tobacco materials has been developed. CA was isolated by polyamide and further purified by silicagel column chromatography. Results reveal that polyamide is suitable for separation of CA from tobacco extract. After one run treatment with polyamide, the content of chlorogenic acids was 40.3%. The product was further purified using silicagel column chromatography; the content of total chlorogenic acid was increased 2.29-fold from 40.3 to 92.2%. The LC-MS results showed that total chlorogenic acids were made up of four components: 5-CQA, 3-CQA, 4-CQA and cis-5-CQA. The content of 5-CQA was the most (78.2%) and other three chlorogenic acid derivatives were 1.9, 10.1 and 2.0%, respectively.

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Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro

Cited by 66 publications

References 37 publications

In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits

In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits

Optimation of Enrichment and Purification of Polyphenols From Blueberries (Vaccinium Spp.) by Macroporous Resins Xad-7hp

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

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