Enrichment of aliphatic, alicyclic and aromatic acids by oil-degrading bacteria isolated from the rhizosphere of plants growing in oil-contaminated soil from Kazakhstan

Abstract

Three microbial strains were isolated from the rhizosphere of alfalfa (Medicago sativa), grass mixture (Festuca rubra, 75 %; Lolium perenne, 20 %; Poa pratensis, 10 %), and rape (Brassica napus) on the basis of their high capacity to use crude oil as the sole carbon and energy source. These isolates used an unusually wide spectrum of hydrocarbons as substrates (more than 80), including n-alkanes with chain lengths ranging from C12 to C32, monomethyl- and monoethyl-substituted alkanes (C12-C23), n-alkylcyclo alkanes with alkyl chain lengths from 4 to 18 carbon atoms, as well as substituted monoaromatic and diaromatic hydrocarbons. These three strains were identified as Gordonia rubripertincta and Rhodococcus sp. SBUG 1968. During their transformation of this wide range of hydrocarbon substrates, a very large number of aliphatic, alicyclic, and aromatic acids was detected, 44 of them were identified by GC/MS analyses, and 4 of them are described as metabolites for the first time. Inoculation of plant seeds with these highly potent bacteria had a beneficial effect on shoot and root development of plants which were grown on oil-contaminated sand.

From oil spills to barley growth – oil‐degrading soil bacteria and their promoting effects

“…Potent oil-degrading species of G. rubripertincta and of Rhodococcus sp. isolated from the Uzen deposit in the Mangystau region have been described previously [64]. However, good growth of Gordonia and Rhodococcus species on oil components and different hydrocarbons is not surprising.…”

“…Several hydrocarbon utilizing gordoniae among them G. ajoucoccus , G . alkanivorans, G. amicalis , G. hirsuta , G. paraffinivorans , G. rubripertincta , G. terrae , and unspecified species of Gordonia as well as Rhodococcus aetherivorans , R. baikonurensis , R. equi , R. erythropolis , R. fascians , R. phenolicus , R. rhodochrous , R. ruber , R. wratislaviensis , and other unspecified rhodococci have been isolated from oil‐contaminated sludge, sediments, sea‐ and stratal water, sand and soil . The contaminated samples were taken from oilfields in Tartastan, western Siberia, different parts of China, South Korea, and Kuwait, from offshore oil and gas platforms in the North Atlantic (Canada), from oil‐contaminated sediments and seawater in the Persian Gulf, in the Caspian Sea and of the northern coast of Tunisia and from oil‐contaminated areas of northeast Brazil, of southeast Mexico, of the west coast of Sicily (Italy), of different sites of France, of central Germany, of central and south Taiwan, of different parts of Korea and Japan, of the western portion of Pampanga (Philippines) and of different sites of Antarctica.…”

Nitrogen-Deficiency Stress Induces Protein Expression Differentially in Low-N Tolerant and Low-N Sensitive Maize Genotypes

“…Once the coleoptiles emerged, plants were transferred to plastic containers with thermocol sheet (2 inch thickness). Each container was filled with 10 L of Standardized Hoagland's solution (half strength; Hoagland and Arnon, 1950 ) for a period of 3 days initially and was replaced by full-strength solution afterwards. Fresh solution was added every 3 days until appearance of the deficiency symptoms.…”

Bacterial endophytes of Trans-Ili Alatau region's plants as promising components of a microbial preparation for agricultural use.

“…The washed roots were then suspended in physiological saline, and the number of microorganisms was counted on the PDA solid medium (34-35). As control, the Gordonia rubripertincta L-RP20 strain was used (Mikolasch et al, 2015).…”