Enrichment of cancer stem-like cells by controlling oxygen, glucose and fluid shear stress in a microfluidic spheroid culture device

Barisam, Maryam; Niavol, Fazeleh Ranjbar; Kinj, Moslem Afrasiabi; Saidi, Mohammad R.; Ghanbarian, Hossein; Kashaninejad, Navid

doi:10.1016/j.jsamd.2022.100439

Cited by 10 publications

(11 citation statements)

References 42 publications

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“…With this device, 100 μm diameter MCF-7 breast cancer spheroids were produced in 2 days of culture, and spheroids’ sizes could be adjusted using different cell concentrations. A microfluidic device with U-shaped arrays was also utilized for a 3D cell culture of A549 cells under a continuous flow of culture medium . With this device, 16 spheroids can be produced in 72 h.…”

Section: Spheroid Engineering In Microfluidic Systemsmentioning

confidence: 99%

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Spheroid Engineering in Microfluidic Devices

et al. 2023

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cell culture techniques are commonly employed to investigate biophysical and biochemical cellular responses. However, these culture methods, having monolayer cells, lack cell−cell and cell−extracellular matrix interactions, mimicking the cell microenvironment and multicellular organization. Three-dimensional (3D) cell culture methods enable equal transportation of nutrients, gas, and growth factors among cells and their microenvironment. Therefore, 3D cultures show similar cell proliferation, apoptosis, and differentiation properties to in vivo. A spheroid is defined as self-assembled 3D cell aggregates, and it closely mimics a cell microenvironment in vitro thanks to cell−cell/matrix interactions, which enables its use in several important applications in medical and clinical research. To fabricate a spheroid, conventional methods such as liquid overlay, hanging drop, and so forth are available. However, these labor-intensive methods result in low-throughput fabrication and uncontrollable spheroid sizes. On the other hand, microfluidic methods enable inexpensive and rapid fabrication of spheroids with high precision. Furthermore, fabricated spheroids can also be cultured in microfluidic devices for controllable cell perfusion, simulation of fluid shear effects, and mimicking of the microenvironment-like in vivo conditions. This review focuses on recent microfluidic spheroid fabrication techniques and also organ-on-a-chip applications of spheroids, which are used in different disease modeling and drug development studies.

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Section: Spheroid Engineering In Microfluidic Systemsmentioning

confidence: 99%

“…A microfluidic device with U-shaped arrays was also utilized for a 3D cell culture of A549 cells under a continuous flow of culture medium. 112 With this device, 16 spheroids can be produced in 72 h.…”

Section: Spheroid Engineering In Microfluidic Systemsmentioning

confidence: 99%

Spheroid Engineering in Microfluidic Devices

et al. 2023

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“…A microfluidic platform with U-shaped barriers was developed by Barisam et al to study the influence of the environmental conditions on in vitro cultures. 39 The U-shaped structures trap the cells and form spheroids, while the microfluidic channel surrounds the spheroids. Using this platform, the research team studied shear stress, hypoxia, glucose metabolism, and anoikis through gene expression from the spheroids.…”

Section: Microfluidic Devicesmentioning

confidence: 99%

Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Kim

Cho

2022

Analyst

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“…These organotypic spheroids are small tumor fragments from human or mouse tissue generated by dissociating tissue specimens into fragments, typically ~100 μm in diameter. Organotypic tumor spheroids retain the native tumor stroma as well as many cell types, and lymphocyte populations can be maintained by supplementing IL-2 to sustain their growth within the spheroids or by adding PBMC or tumor-infiltrating lymphocytes derived from the same sample [ 32 , 33 , 34 , 35 , 36 , 37 , 38 ].…”

Section: Microphysiological Models For Cell Therapy Testingmentioning

confidence: 99%

Engineered Microphysiological Systems for Testing Effectiveness of Cell-Based Cancer Immunotherapies

Shelton

Chen

Kamm

et al. 2022

Cancers

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Cell therapies, including adoptive immune cell therapies and genetically engineered chimeric antigen receptor (CAR) T or NK cells, have shown promise in treating hematologic malignancies. Yet, immune cell infiltration and expansion has proven challenging in solid tumors due to immune cell exclusion and exhaustion and the presence of vascular barriers. Testing next-generation immune therapies remains challenging in animals, motivating sophisticated ex vivo models of human tumor biology and prognostic assays to predict treatment response in real-time while comprehensively recapitulating the human tumor immune microenvironment (TIME). This review examines current strategies for testing cell-based cancer immunotherapies using ex vivo microphysiological systems and microfluidic technologies. Insights into the multicellular interactions of the TIME will identify novel therapeutic strategies to help patients whose tumors are refractory or resistant to current immunotherapies. Altogether, these microphysiological systems (MPS) have the capability to predict therapeutic vulnerabilities and biological barriers while studying immune cell infiltration and killing in a more physiologically relevant context, thereby providing important insights into fundamental biologic mechanisms to expand our understanding of and treatments for currently incurable malignancies.

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Enrichment of cancer stem-like cells by controlling oxygen, glucose and fluid shear stress in a microfluidic spheroid culture device

Cited by 10 publications

References 42 publications

Spheroid Engineering in Microfluidic Devices

Spheroid Engineering in Microfluidic Devices

Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Engineered Microphysiological Systems for Testing Effectiveness of Cell-Based Cancer Immunotherapies

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