Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Ro, Jooyoung; Kim, Junyoung; Cho, Yoon‐Kyoung

doi:10.1039/d2an00172a

Cited by 19 publications

(10 citation statements)

References 76 publications

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“…Interactions between tumor spheroids and neighboring stromal cells, such as endothelial, mesenchymal, and fibroblast cells, contribute to tumorigenesis, metastasis, and drug resistance. 151 To mimic this type of cell-to-cell interaction in vitro, Chung et al 152 developed a tree-branched droplet microfluidic chip to fabricate spheroids containing different ratios of MCF-7 and NIH-3T3 fibroblast cells (Figure 6b). The microfluidic chip contained two inlets for injecting suspensions of MCF-7 and NIH-3T3 cells.…”

Section: D Cellmentioning

confidence: 99%

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Droplet Microfluidics for Current Cancer Research: From Single-Cell Analysis to 3D Cell Culture

Jiang,

Guo,

Chen

et al. 2024

ACS Biomater. Sci. Eng.

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Cancer is the second leading cause of death worldwide. Differences in drug resistance and treatment response caused by the heterogeneity of cancer cells are the primary reasons for poor cancer therapy outcomes in patients. In addition, current in vitro anticancer drug-screening methods rely on two-dimensional monolayer-cultured cancer cells, which cannot accurately predict drug behavior in vivo. Therefore, a powerful tool to study the heterogeneity of cancer cells and produce effective in vitro tumor models is warranted to leverage cancer research. Droplet microfluidics has become a powerful platform for the single-cell analysis of cancer cells and three-dimensional cell culture of in vitro tumor spheroids. In this review, we discuss the use of droplet microfluidics in cancer research. Droplet microfluidic technologies, including single-or double-emulsion droplet generation and passive-or active-droplet manipulation, are concisely discussed. Recent advances in droplet microfluidics for single-cell analysis of cancer cells, circulating tumor cells, and scaffold-free/based 3D cell culture of tumor spheroids have been systematically introduced. Finally, the challenges that must be overcome for the further application of droplet microfluidics in cancer research are discussed.

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Section: D Cellmentioning

confidence: 99%

Section: Applications In Current Cancer Researchmentioning

confidence: 99%

Droplet Microfluidics for Current Cancer Research: From Single-Cell Analysis to 3D Cell Culture

Jiang,

Guo,

Chen

et al. 2024

ACS Biomater. Sci. Eng.

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“…The use of these hydrogels allows for high throughput fabrication of readily adaptable and physiologically relevant environments, making it appealing for the study of ECM for cancer proliferation and invasion. Other approaches to mimicking the 3D microenvironment include 3D bioprinting which is beneficial in creating an exact replication of target tissue with consideration for cellular components, ECM and 3D spatial components (for reviews see; ( Charbe et al, 2017 ; Ro et al, 2022 )). Alternatively, the decellularization of tumors also offers a new Frontier in tissue engineering that maintains not only the mechanical components of the ECM but also the composition and structure (for review see; ( García-Gareta et al, 2022 )).…”

Section: Tools For Recapitulating the Tumor Microenvironmentmentioning

confidence: 99%

Modelling the Tumor Microenvironment: Recapitulating Nano- and Micro-Scale Properties that Regulate Tumor Progression

Vahala

Choi

2022

Front. Cell Dev. Biol.

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Breast cancer remains a significant burden with 1 in 8 women affected and metastasis posing a significant challenge for patient survival. Disease progression involves remodeling of the extracellular matrix (ECM). In breast cancer, tissue stiffness increases owing to an increase in collagen production by recruited cancer-associated fibroblasts (CAFs). These stromal modifications are notable during primary tumor growth and have a dualistic action by creating a hard capsule to prevent penetration of anti-cancer therapies and forming a favorable environment for tumor progression. Remodeling of the tumor microenvironment immediately presented to cells can include changes in protein composition, concentration and structural arrangement and provides the first mechanical stimuli in the metastatic cascade. Not surprisingly, metastatic cancer cells possess the ability to mechanically adapt, and their adaptability ensures not only survival but successful invasion within altered environments. In the past decade, the importance of the microenvironment and its regulatory role in diseases have gained traction and this is evident in the shift from plastic culture to the development of novel biomaterials that mimic in vivo tissue. With these advances, elucidations can be made into how ECM remodeling and more specifically, altered cell-ECM adhesions, regulate tumor growth and cancer cell plasticity. Such enabling tools in mechanobiology will identify fundamental mechanisms in cancer progression that eventually help develop preventative and therapeutic treatment from a clinical perspective. This review will focus on current platforms engineered to mimic the micro and nano-properties of the tumor microenvironment and subsequent understanding of mechanically regulated pathways in cancer.

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“…For the past decade, formation of multicellular clusters known as spheroids has been among the most popular strategies for 3D cell culture. [1][2][3][4] Control of stem cell differentiation has been achieved using tissue-like organoids that can recapitulate the developmental fields of multicellular organoids. 5,6 To better model the responses of cells to drug candidates, organotypic multicellular spheroids have also been created.…”

Section: Introductionmentioning

confidence: 99%

A multiscale, vertical-flow perfusion system with integrated porous microchambers for upgrading multicellular spheroid culture

et al. 2023

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Recent advances in spheroid-based microfluidic models to mimic the tumour microenvironment

Abstract: Three-dimensional (3D) multicellular spheroid models can recapitulate the human tumour microenvironment with more accuracy than conventional cell culture models, as they include complex architectural structures and dynamic cellular interactions. Among...

Cited by 19 publications

References 76 publications

Droplet Microfluidics for Current Cancer Research: From Single-Cell Analysis to 3D Cell Culture

Droplet Microfluidics for Current Cancer Research: From Single-Cell Analysis to 3D Cell Culture

Modelling the Tumor Microenvironment: Recapitulating Nano- and Micro-Scale Properties that Regulate Tumor Progression

A multiscale, vertical-flow perfusion system with integrated porous microchambers for upgrading multicellular spheroid culture

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