Enrichment of Clostridia enhances Geobacter population and electron harvesting in a complex electroactive biofilm

Rivalland, Caroline; Radouani, Fatima; Gonzalez‐Rizzo, Silvina; Robert, Florent; Salvin, Paule

doi:10.1016/j.bioelechem.2021.107954

Cited by 25 publications

(8 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…that utilizes H 2 was predominant in MFCs 76 . The results endorse the existing hypothesis that a pluralistic microbial community as the anodic biocatalyst in MFCs contributes to improving electrical power from complex fuel sources 11 . The studies on the correlation between functional profiles and electrogenic behaviors of bacteria are limited.…”

Section: Discussionsupporting

confidence: 87%

“…Definitely, Clostridium sp. has been progressively recognized as a dominating species in MFCs 11 , 70 , 71 . The relative abundance of Lactobacillus decreased from 5.47% in the coated anode (S4) sample to 2.185% in the uncoated anode (S1) sample.…”

Section: Discussionmentioning

confidence: 99%

“…A plethora of scientific reports are available on developing electrogenic microbial biofilm on electrode surfaces for harvesting power in MFC 8 , 9 . Electrogens are electrochemically active microbes, most commonly bacteria, which produce electrical energy in an MFC setup by degrading organic compounds and transferring the generated electrons to an electrode 10 , 11 . The biofilm formation of these electrogens on the electrode (mostly anode) surface is a prerequisite for harvesting sufficient metabolic electrons from the oxidation of organic compounds in the wastewater for generating desired power in MFCs 12 , 13 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell

Hemdan

El‐Taweel

Naha

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Formation of electrogenic microbial biofilm on the electrode is critical for harvesting electrical power from wastewater in microbial biofuel cells (MFCs). Although the knowledge of bacterial community structures in the biofilm is vital for the rational design of MFC electrodes, an in-depth study on the subject is still awaiting. Herein, we attempt to address this issue by creating electrogenic biofilm on modified graphite anodes assembled in an air–cathode MFC. The modification was performed with reduced graphene oxide (rGO), polyaniline (PANI), and carbon nanotube (CNTs) separately. To accelerate the growth of the biofilm, soybean-potato composite (plant) powder was blended with these conductive materials during the fabrication of the anodes. The MFC fabricated with PANI-based anode delivered the current density of 324.2 mA cm−2, followed by CNTs (248.75 mA cm−2), rGO (193 mA cm−2), and blank (without coating) (151 mA cm−2) graphite electrodes. Likewise, the PANI-based anode supported a robust biofilm growth containing maximum bacterial cell densities with diverse shapes and sizes of the cells and broad metabolic functionality. The alpha diversity of the biofilm developed over the anode coated with PANI was the loftiest operational taxonomic unit (2058 OUT) and Shannon index (7.56), as disclosed from the high-throughput 16S rRNA sequence analysis. Further, within these taxonomic units, exoelectrogenic phyla comprising Proteobacteria, Firmicutes, and Bacteroidetes were maximum with their corresponding level (%) 45.5, 36.2, and 9.8. The relative abundance of Gammaproteobacteria, Clostridia, and Bacilli at the class level, while Pseudomonas, Clostridium, Enterococcus, and Bifidobacterium at the genus level were comparatively higher in the PANI-based anode.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell

Hemdan

El‐Taweel

Naha

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Today, the number of isolated electroactive bacteria is increasing. Therefore, the number of bacteria that are found to be capable of carrying out direct or indirect electron transfer is also increasing [ 19 , 20 , 21 , 22 , 23 ]. This knowledge broadens the spectrum of known electrogenic bacteria with different metabolic and enzymatic capacities, and based on that knowledge, special microbial consortia can be established that can degrade simple [ 24 , 25 ] or more complex substrates, such as cellulose, lignin or proteins [ 11 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterisation of Electrogenic Bacteria from Mud Samples

et al. 2023

View full text Add to dashboard Cite

To develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation capacities and macromolecule degradation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identifications have revealed that isolates represented 18 known and 4 unknown genuses. They all had the capacities to reduce the Reactive Black 5 stain in the agar medium, and 48 of them were positive in the wolfram nanorod reduction assay. The isolates formed biofilm to different extents on the surfaces of both adhesive and non-adhesive 96-well polystyrene plates and glass. Scanning electron microscopy images revealed the different adhesion potentials of isolates to the surface of carbon tissue fibres. Eight of them (15%) were able to form massive amounts of biofilm in three days at 23 °C. A total of 70% of the isolates produced proteases, while lipase and amylase production was lower, at 38% and 27% respectively. All of the macromolecule-degrading enzymes were produced by 11 isolates, and two isolates of them had the capacity to form a strong biofilm on the carbon tissue one of the most used anodic materials in MFC systems. This study discusses the potential of the isolates for future MFC development applications.

show abstract

“…The published version will differ from this one as a result of linguistic and technical corrections and layout editing. 4 (18,19). The use of a design consortium is a developing research topic in metabolic engineering.…”

Section: Introductionmentioning

confidence: 99%

Biohydrogen Production in Microbial Electrolysis Cell Operating on Designed Consortium of Denitrifying Bacteria

Ekadewi

Arbianti

Gómez

et al. 2023

Food Technol. Biotechnol. (Online)

View full text Add to dashboard Cite

Research background. This study provides insight into the use of a designed microbial community to produce biohydrogen in simple, single-chambered microbial electrolysis cells (MECs). The ability of MECs to stably produce biohydrogen relies heavily on the setup and microorganisms working inside the system. Despite having the most straightforward configuration and effectively avoiding costly membranes, single-chambered MECs are prone to competing metabolic pathways. We present in this study one possible way of avoiding this problem using characteristically defined, designed microbial consortia. In this study, we are comparing the performance of MECs inoculated with a design consortium to MECs operating with a naturally occurring soil consortium. Experimental approach. We adapted a cost-efficient and simple single-chambered MEC design. The MEC was gas-tight, 100 mL in volume, and equipped with continuous monitoring for electrical output using a digital multimeter. Microorganisms were sourced from Indonesian environmental samples, either as denitrifying bacterial isolates grouped as a design consortium or natural soil microbiome used in its entirety. The design consortium consisted of 5 species spread among the Pseudomonas and Acinetobacter genera. The headspace gas profile was followed periodically by gas chromatography. At the end of the culture, the composition of the natural soil consortium was characterized by NGS and the anodes were observed for surface-growth of the bacteria by FE-SEM imaging. Results and conclusions. We found that MEC operating on a design consortium presented a better H2 production profile, with the ability of the system to maintain headspace H2 concentration relatively stable for a long time after reaching stationary growth period. In contrast, MECs inoculated with soil microbiome exhibited a strong decline in headspace H2 profile within the same timeframe. Novelty and scientific contribution. This work utilizes a designed, denitrifying bacterial consortium isolated from Indonesian environmental samples that can survive in a nitrate-rich environment. Here we propose using a designed consortium as a biological approach to avoid methanogenesis in MECs, as a simple and environmentally friendly alternative to current chemical/physical methods. Our findings offer an alternative solution to avoid the problem of H2 loss in single-chambered MECs along with optimizing biohydrogen production through bioelectrochemical routes.

show abstract

Enrichment of Clostridia enhances Geobacter population and electron harvesting in a complex electroactive biofilm

Cited by 25 publications

References 52 publications

Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell

Bacterial community structure of electrogenic biofilm developed on modified graphite anode in microbial fuel cell

Isolation and Characterisation of Electrogenic Bacteria from Mud Samples

Biohydrogen Production in Microbial Electrolysis Cell Operating on Designed Consortium of Denitrifying Bacteria

Contact Info

Product

Resources

About