Enrichment of rare cancer cells through depletion of normal cells using density and flow-through, immunomagnetic cell separation

Lara, Oscar; Tong, Xiaodong; Zborowski, Maciej; Chalmers, Jeffrey J.

doi:10.1016/j.exphem.2004.07.007

Cited by 183 publications

(176 citation statements)

References 47 publications

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“…Negative enrichment technologies evade some of the pitfalls of positive enrichment; for example, CTCs are not tagged with a difficult-to-remove antibody, and antibody selection does not bias the subpopulation of CTCs captured. However, these advantages come at the cost of purity, as negative enrichment strategies typically have a much lower purity than positive enrichment (Baccelli et al, 2013;Lara et al, 2004;Yang et al, 2009).…”

Section: Vortex Sizementioning

confidence: 99%

“…While they generally do not achieve the same high purity levels as positive enrichment, depletion methods may be preferred for some studies, as they do not bias the sample according to selection markers or apply difficult-to-remove labels. Two negative enrichment platforms that researchers have used to isolate CTCs from clinical samples include the commercialized EasySep Ò system (STEMCELL Technologies, Vancouver, Canada) and the Quadrupole Magnetic Separator (QMS) (Lara et al, 2004;Wu et al, 2013). The EasySepä system immunomagnetically depletes unwanted cells by first incubating samples with the EasySepä Human CD45 Depletion kit, which contains magnetic nanoparticles and tetrameric antibody complexes targeting CD45.…”

Section: Negative Enrichment Technologiesmentioning

confidence: 99%

“…Simple, easy-to-use batch separation QMS Continuous flow, high-throughput (Lara et al, 2004;Wu et al, 2013) MACS (Giordano et al, 2012;Lara et al, 2006) mF, IM CTC-iChip CD45, CD66b, Size (Karabacak et al, 2014;Ozkumur et al, 2013) Biophysical e Density Gradient Centrifugation Sample placed on top of a separation medium and centrifuged to separate different cell populations based on their relative densities.…”

Section: Cd45mentioning

confidence: 99%

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Circulating tumor cell technologies

2016

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Cancer CTC capture CTC clustersImmunoaffinity enrichment A B S T R A C TCirculating tumor cells, a component of the "liquid biopsy", hold great potential to transform the current landscape of cancer therapy. A key challenge to unlocking the clinical utility of CTCs lies in the ability to detect and isolate these rare cells using methods amenable to downstream characterization and other applications. In this review, we will provide an overview of current technologies used to detect and capture CTCs with brief insights into the workings of individual technologies. We focus on the strategies employed by different platforms and discuss the advantages of each. As our understanding of CTC biology matures, CTC technologies will need to evolve, and we discuss some of the present challenges facing the field in light of recent data encompassing epithelial-to-mesenchymal transition, tumor-initiating cells, and CTC clusters.

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Section: Vortex Sizementioning

confidence: 99%

Section: Negative Enrichment Technologiesmentioning

confidence: 99%

Section: Cd45mentioning

confidence: 99%

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Circulating tumor cell technologies

2016

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“…Recent magnetic separation technology development has led to such diverse applications as cancer progression monitoring by separation of circulating tumor cells (1)(2)(3)(4)(5)(6) and reconstitution of hematopoiesis in patients having undergone chemotherapy (7)(8)(9)(10)(11).…”

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confidence: 99%

Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies

et al. 2005

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Background: Continuous flow immunomagnetic separation is an attractive alternative to current batch mode immunomagnetic separation methods because it is capable of high sorting speeds at mild cell conditions, and grants the operator better control of separation process. The control of the separation is dependent on knowledge of the amount of magnetic label attached to the cell (magnetic labeling intensity), however. Determination of the magnetic labeling is accomplished by measuring cell magnetophoretic mobility using a newly developed technique of Cell Tracking Velocimetry (CTV). Methods: Flow cytometry was used to define the antibody binding characteristics of a fluorescently tagged primary antibody. Subsequently, CTV was used to measure antibody‐binding characteristics of a magnetically tagged secondary antibody. Results: The results of this study show that CTV is capable of providing valuable information concerning the cell labeling by magnetically tagged antibodies. It was demonstrated that the magnetically conjugated antibody binding curve exhibits the same exponential increase to saturation characteristics as that seen with the fluorescently tagged antibody. Further, it was shown that the intensity of the secondary magnetic labeling is directly proportional to the intensity of the primary fluorescent label. Conclusions: CTV is an accurate tool for evaluation of magnetically conjugated antibodies. The ability to determine the intensity of magnetic labeling is necessary for the development of continuous flow immunomagnetic separations based on cell magnetophoresis. © 2005 Wiley‐Liss, Inc.

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“…This is especially important in low concentration bio-samples. [13][14][15] The number of rings in the conducting structure can be customized according to the size of the area, from which the MPs need to be attracted, trapped, and transported without the need to increase the number of switches or to unduly increase the complexity of the fabrication. By grouping the rings into sets the same number of switches can be used.…”

Section: An Integrated Micro-chip For Rapid Detection Of Magnetic Parmentioning

confidence: 99%

An integrated micro-chip for rapid detection of magnetic particles

Gooneratne

Cai

Giouroudi

et al. 2012

Journal of Applied Physics

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This paper proposes an integrated micro-chip for the manipulation and detection of magnetic particles (MPs). A conducting ring structure is used to manipulate MPs toward giant magnetoresistance (GMR) sensing elements for rapid detection. The GMR sensor is fabricated in a horseshoe shape in order to detect the majority of MPs that are trapped around the conducting structure. The GMR sensing elements are connected in a Wheatstone bridge circuit topology for optimum noise suppression. Full fabrication details of the micro-chip, characterization of the GMR sensors, and experimental results with MPs are presented in this paper. Experimental results showed that the micro-chip can detect MPs from low concentration samples after they were guided toward the GMR sensors by applying current to the conducting ring structure.

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Enrichment of rare cancer cells through depletion of normal cells using density and flow-through, immunomagnetic cell separation

Cited by 183 publications

References 47 publications

Circulating tumor cell technologies

Circulating tumor cell technologies

Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies

An integrated micro-chip for rapid detection of magnetic particles

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