Ensuring seafood identity: Grouper identification by real-time nucleic acid sequence-based amplification (RT-NASBA)

Ulrich, Robert M.; John, David E.; Barton, Gregg H.; Hendrick, Gary S.; Fries, David; Paul, John H.

doi:10.1016/j.foodcont.2012.11.012

Cited by 10 publications

(19 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the traditional tools for the control and regulation of fisheries are extremely limited, and the modern methods available for the identification of epinephelid species, such as real-time PCR (Chen et al, 2012;Ulrich et al, 2013) and DNA barcoding which, while effective, demand expensive equipment and laboratory resources. In addition, no molecular identification procedure is currently in use for the detection of the illegal fishing of the Atlantic goliath grouper in Brazil.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular markers used in the multiplex fingerprinting technique for the authentication, control, and inspection of fishery stocks are based on the design of specific oligonucleotides and markers derived from the sequencebased amplification of the 5S, 12S, 18S (Asensio, 2008, Asensio et al, 2009, 16S (Ulrich et al, 2013), Cyt b (Melo Palmeira et al, 2013), and COI genes , as well as fragment length polymorphisms, RFLPs (Paine et al 2007;Torres et al 2013) or even nuclear markers, such as ITS 2 (Feldheim et al, 2010), SNPs (Torres et al, 2013) and microsatellites (Rodrigues et al, 2011), and realtime PCR techniques (Trotta et al, 2005). The COI gene is a highly effective and reliable tool for the diagnosis of the authenticity of fishery products (Veneza et al, 2014), and the multiplex PCR technique has proven especially effective for the detection of endangered species De-Franco et al, 2010;Alexandre de-Franco et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Genetic tools for the molecular identification of a number of different fish species have been developed during the past decade (Ward et al, 2005;Craig et al, 2009;Hashimoto et al, 2010;Rodrigues et al, 2011;Alexandre de-Franco et al, 2012;Ulrich et al, 2013;Veneza et al, 2014). Carvalho et al (2015) has shown the DNA barcoding to be an effective molecular tool for the detection of commercially-valuable species such as the groupers, while Torres et al (2013) successfully applied PCR RFLP and SNP detection techniques to the identification of specimens of E. itajara, E. morio, M. bonaci, and M. marginata.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR

et al. 2016

View full text Add to dashboard Cite

The Atlantic goliath grouper, Epinephelus itajara, is a critically endangered species, threatened by illegal fishing and the destruction of its habitats. A number of other closely related grouper species found in the western Atlantic are also fished intensively. While some countries apply rigorous legislation, illegal harvesting followed by the falsification of fish products, which impedes the correct identification of the species, is a common practice, allowing the catch to be marketed as a different grouper species. In this case, molecular techniques represent an important tool for the monitoring and regulation of fishery practices, and are essential for the forensic identification of a number of different species. In the present study, species-specific primers were developed for the Cytochrome Oxidase subunit I gene, which were applied in a multiplex PCR for the simultaneous identification of nine different species of Epinephelidae: Epinephelus itajara, E. quinquefasciatus, E. morio, Hyporthodus flavolimbatus, H. niveatus, Mycteroperca acutirostris, M. bonaci, M. marginata, and M. microlepis.Multiplex PCR is a rapid, reliable and cost-effective procedure for the identification of commercially-valuable endangered fish species, and may represent a valuable tool for the regulation and sustainable management of fishery resources.O mero, Epinephelus itajara, encontra-se criticamente ameaçado, resultado da pesca ilegal e destruição dos habitas. Filogeneticamente relacionadas a este táxon encontram-se garoupas que atualmente são intensamente pescadas no Atlântico Oeste. Apesar de leis mais restritivas aplicadas em alguns países, a captura ilegal com a descaracterização morfológica é uma prática comum que impossibilita a identificação correta da espécie permitindo que seja comercializada como garoupas, badejos ou chernes. A este respeito, técnicas moleculares representam ferramentas importantes para o monitoramento e fiscalização da pesca, provando ser essencial, na identificação forense de diversas espécies. Primers espécie-específicos foram desenvolvidos com base no gene Citocromo Oxidase subunidade I que aplicados em PCR-Multiplex possibilitam a identificação simultânea de nove espécies Epinephelidae: Epinephelus itajara, E. quinquefasciatus, E. morio, Hyporthodus flavolimbatus, H. niveatus, Mycteroperca acutirostris, M. bonaci, M. marginata e M. microlepis. A identificação via PCR multiplex de espécies de peixes ameaçadas e comercialmente importantes é um método rápido, prático, seguro e de baixo custo, que poderá ser útil o controle do uso e manejo sustentável de recursos pesqueiros.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR

et al. 2016

View full text Add to dashboard Cite

show abstract

“…We have previously developed a real-time nucleic acid sequence-based amplification assay (RT-NASBA) that is able to differentiate most of the FDA allowable groupers from potential surrogate fish species by targeting a region of the mitochondrial 16S rDNA gene (Ulrich et al, 2013). NASBA is an isothermal RNA amplification method that, when used in conjunction with fluorescently-labeled molecular beacons, provides real-time identification of specific nucleotide sequences (Compton, 1991;Tyagi & Kramer, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…We believe this technology will provide a useful on-site screening tool to aid seafood processors, distributors, retailers and restaurateurs in remaining compliant with compulsory FDA regulations on salable grouper species. Grouper specimens from which samples were taken were identified by phenotypic characterization by each of the above organizations, and were confirmed by in-house DNA sequence analysis performed by members of the Paul Lab at USF as described in Ulrich et al (2013). Any specimen designated as vouchered underwent more rigorous phenotypic authentication protocols performed by taxonomists at respective institutions.…”

Section: Introductionmentioning

confidence: 99%