Enteral broad-spectrum antibiotics antagonize the effect of fecal microbiota transplantation in preterm pigs

Brunse, Anders; Mosegaard, Josefine Juliane; Deng, Ling; Damborg, Peter; Nielsen, Dennis Sandris; Sangild, Per Torp; Thymann, Thomas; Nguyen, Duc Ninh

doi:10.1080/19490976.2020.1849997

Cited by 18 publications

(13 citation statements)

References 41 publications

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“…There were several novel scientific projects that could be further studied based on our results. Considering the similar bacterial community of goats rumen were identified in the colon of mice recipient, a developed RMT approach through colon perfusion or rectal inoculation 41 could be further tested in the future study and may serve as a feasible and effective method to test the ruminal microbial roles in mice model. Furthermore, the previous study has also reported the goats with acidosis susceptible or acidosis resistance when high grain diet was supplied 42 , which has not been studied in the present study.…”

Section: Discussionmentioning

confidence: 99%

Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis

Chen

et al. 2021

npj Biofilms Microbiomes

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Ruminal microbiota changes frequently with high grain diets and the occurrence of subacute ruminal acidosis (SARA). A grain-induced goat model of SARA, with durations of a significant decrease in the rumen pH value to less than 5.6 and an increase in the rumen lipopolysaccharides concentration, is constructed for real-time monitoring of bacteria alteration. Using 16 S rRNA gene sequencing, significant bacterial differences between goats from the SARA and healthy groups are identified at every hour for six continuous hours after feeding. Moreover, 29 common differential genera between two groups over 6 h after feeding are all related to the altered pH and lipopolysaccharides. Transplanting the microbiota from donor goats with SARA could induce colonic inflammation in antibiotic-pretreated mice. Overall, significant differences in the bacterial community and rumen fermentation pattern between the healthy and SARA dairy goats are real-time monitored, and then tested using ruminal microbe transplantation to antibiotic-treated mice.

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Section: Discussionmentioning

confidence: 99%

Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis

Chen

et al. 2021

npj Biofilms Microbiomes

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“…All piglets (cross-bred, Landrace x Yorkshire x Duroc) were delivered by elective cesarean section at gestational day 106 corresponding to ∼90% of the total length of gestation. Sow’s anesthesia and surgical procedures are described in details elsewhere (44). After delivery, the animals were single-housed in ventilated, heated (37°C) incubators with oxygen supply (1 l/min).…”

Section: Methodsmentioning

confidence: 99%

Parenteral glucose supply and pharmacological glycolysis inhibition determine the clinical fate of infected preterm newborns

Muk

Brunse

Henriksen

et al. 2021

Preprint

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Preterm infants are susceptible to bloodstream infection that can lead to sepsis. High parenteral glucose supplement is commonly used to support their growth and energy expenditure, but may exceed endogenous regulation during infection, causing dysregulated immune response and clinical deterioration. Using a preterm piglet model of neonatal sepsis induced by Staphylococcus epidermidis infection, we demonstrate the delicate interplay between immunity and energy metabolism to regulate the host infection response. Circulating glucose levels, glycolysis and inflammatory response to infection are closely connected across the states of tolerance, resistance and immunoparalysis. Further, high parenteral glucose provision during infection induces hyperglycemia, elevated glycolysis and inflammation, leading to lactate acidosis and sepsis, whereas glucose restricted individuals are clinically unaffected with increased gluconeogenesis to maintain moderate hypoglycemia. Finally, pharmacological glycolysis inhibition during normoglycemia enhances bacterial clearance and dampens inflammation but fails to prevent sepsis. Our results uncover how blood glucose controls immune cell metabolism and function, in turn determining the clinical fate of infected preterm neonates. This also questions the current practice of parenteral glucose supply for infected preterm infants.

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Section: The Pig Microbiomementioning

confidence: 99%

“…It has been hypothesized that a fecal matter transplant (FMT) from a healthy donor could help restore the microbiota after antibiotic treatment [66]. However, antibiotic treatment prior to FMT could undermine the beneficial effect of FMT in piglets [66]. In fact, FMT received after an antibiotic treatment did not restore the immune response or the level of antibiotic resistance in the microbiota to match that of the group that only received FMT.…”

Section: The Pig Microbiomementioning

confidence: 99%

Antibiotic Resistance: From Pig to Meat

et al. 2021

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Pork meat is in high demand worldwide and this is expected to increase. Pork is often raised in intensive conditions, which is conducive to the spread of infectious diseases. Vaccines, antibiotics, and other biosafety measures help mitigate the impact of infectious diseases. However, bacterial strains resistant to antibiotics are more and more frequently found in pig farms, animals, and the environment. It is now recognized that a holistic perspective is needed to sustainably fight antibiotic resistance, and that an integrated One Health approach is essential. With this in mind, this review tackles antibiotic resistance throughout the pork raising process, including their microbiome; many factors of their environment (agricultural workers, farms, rivers, etc.); and an overview of the impact of antibiotic resistance on pork meat, which is the end product available to consumers. Antibiotic resistance, while a natural process, is a public health concern. If we react, and act, collectively, it is expected to be, at least partially, reversible with judicious antibiotic usage and the development of innovative strategies and tools to foster animal health.

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Enteral broad-spectrum antibiotics antagonize the effect of fecal microbiota transplantation in preterm pigs

Cited by 18 publications

References 41 publications

Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis

Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis

Parenteral glucose supply and pharmacological glycolysis inhibition determine the clinical fate of infected preterm newborns

Antibiotic Resistance: From Pig to Meat

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