Enteric Campylobacter: Purging Its Secrets?

Crushell, Ellen; Harty, Sinead; Sharif, Farhana; Bourke, Billy

doi:10.1203/01.pdr.0000099794.06260.71

Cited by 90 publications

(90 citation statements)

References 127 publications

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“…Raw material, especially of animal origin, is one of the main sources of microbial contamination in the food sector. In spite of the decline observed between 1999 and 2001 in the European Union, the majority of the cases of food-borne diseases are associated with Salmonella and Campylobacter (O' Brien & de Valk, 2003;Crushell et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

113

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Bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. In the present study, two probiotic strains, Lactobacillus rhamnosus CNCM-I-3698 and Lactobacillus farciminis CNCM-I-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference Lactobacillus strains (Lb. rhamnosus GG and Lb. farciminis CIP 103136). The autoaggregation potential of both forms was higher than that of reference strains and twice that of pathogenic strains. The coaggregation potential of these two beneficial micro-organisms was evaluated against several pathogenic agents that threaten the global safety of the feed/food chain: Escherichia coli, Salmonella spp., Campylobacter spp. and Listeria monocytogenes. The strongest coaggregative interactions were demonstrated with Campylobacter spp. by a coaggregation test, confirmed by electron microscopic examination for the two forms. Viable forms were investigated for the nature of the bacterial cell-surface molecules involved, by sugar reversal tests and chemical and enzymic pretreatments. The results suggest that the coaggregation between both probiotic strains and C. jejuni CIP 70.2 T is mediated by a carbohydrate-lectin interaction. The autoaggregation potential of the two probiotics decreased upon exposure to proteinase, SDS or LiCl, showing that proteinaceous components on the surface of the two lactobacilli play an important role in this interaction. Adhesion abilities of both Lactobacillus strains were also demonstrated at significant levels on Caco-2 cells, mucin and extracellular matrix material. Both viable and heat-killed forms of the two probiotic lactobacilli inhibited the attachment of C. jejuni CIP 70.2 T to mucin. In conclusion, in vitro assays showed that Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, as viable or heat-killed forms, are adherent to different intestinal matrix models and are highly aggregative in vitro with pathogens, especially Campylobacter spp., the most commonly reported zoonotic agent in the European Union. This study supports the need for further in vivo investigations to demonstrate the potential food safety benefits of Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, live or heat-killed, in the global feed/food chain.

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Section: Introductionmentioning

confidence: 99%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

113

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“…Campylobacter are the principal bacterial cause of foodborne gastrointestinal disease in developed countries and probably also in the developing world (Blaser, 1997;Crushell et al, 2004;Takkinen et al, 2003). Additionally, Campylobacter can cause invasive disease and extraintestinal sequelae including bacteraemia and GuillainBarré syndrome (Hannu et al, 2002;Reed et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro

2007

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“…1,5,6 No que respeita aos mecanismos patogénicos envolvidos na GEA, pensa-se que a bactéria lesa as células epiteliais intestinais por invasão direta, por indução de um processo inflamatório e pela produção de uma toxina. 1,[6][7][8][9]12,13 Está habitualmente associado a um reservatório animal (aves, gado bovino, caprino e ovino) e transmite-se maioritariamente pela ingestão de carne mal cozinhada (principalmente de aves), leite não pasteurizado, outros alimentos contaminados e água não tratada.…”

Section: Introductionunclassified

Gastroenterite Aguda por Campylobacter Spp: Casuística de uma Urgência Pediátrica

et al. 2014

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Results: Out of 216 stool cultures, 98 (45%) were positive. We identified Campylobacter in 49 (50%) cases; 30 (61%) were female. Median age was 23 months. Fourteen patients were under one year of age, 25 between one and five years old and 10 patients were over five years old. Watery diarrhea was identified in 5 (10%) patients, bloody diarrhea in 44 (90%) and mucosanguineous in 14 (29%), while 23 (47%) had fever, 14 (29%) complained of abdominal pain and 11 (22%) presented with vomiting. One patient was septic. Five patients were admitted as inpatients. Eight patients were treated with azithromycin. Discussion: This is the largest published national series on gastroenteritis by Campylobacter in children and the first in the south region. Campylobacter was the main bacteria isolated. Infection was self-resolving in the majority of cases. Nevertheless, severe forms of this infection should be considered. Increased resistance to quinolones is worrisome. Conclusion: Judicious use of stool cultures allows etiological identification of bacterial gastroenteritis. The increase in Campylobacter cases reinforces the need for better control of hygiene measures in handling food products.

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Enteric Campylobacter: Purging Its Secrets?

Cited by 90 publications

References 127 publications

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro

Gastroenterite Aguda por Campylobacter Spp: Casuística de uma Urgência Pediátrica

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