Enteritis due to enteropathogenic escherichia coli

Neter, Erwin

doi:10.1016/s0022-3476(59)80091-3

Cited by 82 publications

(17 citation statements)

References 56 publications

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“…3). As in the E. coli group, patients with initial coliform concentrations of less than 3.5 log/ml in the jejunum appeared to (18,19). The disease can be reproduced by feeding EEC to healthy adult volunteers (20,21).…”

Section: Resultsmentioning

confidence: 90%

Acute undifferentiated human diarrhea in the tropics

Gorbach¹,

Banwell²,

Chatterjee³

et al. 1971

J. Clin. Invest.

200

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Section: Resultsmentioning

confidence: 90%

Acute undifferentiated human diarrhea in the tropics

Gorbach¹,

Banwell²,

Chatterjee³

et al. 1971

J. Clin. Invest.

200

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“…Enteropathogenic Escherichia coli (EPEC) is the leading cause of bacterium-mediated infantile diarrhea, killing several hundred thousand children every year (19,30). Natural animal disease models such as rabbits infected with rabbit EPEC serogroup O103 (REPEC O103) have been used to study pathogenesis.…”

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confidence: 99%

Decreased Apoptosis in the Ileum and Ileal Peyer's Patches: a Feature after Infection with Rabbit Enteropathogenic Escherichia coli O103

et al. 2001

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Significant changes occur in intestinal epithelial cells after infection with enteropathogenic Escherichia coli (EPEC). However, it is unclear whether this pathogen alters rates of apoptosis. By using a naturally occurring weaned rabbit infection model, we determined physiological levels of apoptosis in rabbit ileum and ileal Peyer's patches (PP) and compared them to those found after infection with adherent rabbit EPEC (REPEC O103). Various REPEC O103 strains were first tested in vitro for characteristic virulence features. Rabbits were then inoculated with the REPEC O103 strains that infected cultured cells the most efficiently. After experimental infection, intestinal samples were examined by light and electron microscopy. Simultaneously, ileal apoptosis was assessed by using terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) and caspase 3 assays and by apoptotic cell counts based on morphology (hematoxylin-and-eosin staining). The highest physiological apoptotic indices were measured in PP germinal centers (median ‫؍‬ 14.7%), followed by PP domed villi (8.1%), tips of absorptive villi (3.8%), and ileal crypt regions (0.5%). Severe infection with REPEC O103 resulted in a significant decrease in apoptosis in PP germinal centers (determined by TUNEL assay; P ‫؍‬ 0.01), in the tips of ileal absorptive villi (determined by H&E staining; P ‫؍‬ 0.04), and in whole ileal cell lysates (determined by caspase 3 assay; P ‫؍‬ 0.001). We concluded that REPEC O103 does not promote apoptosis. Furthermore, we cannot rule out the possibility that REPEC O103, in fact, decreases apoptotic levels in the rabbit ileum.

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“…Breast-fed babies are relatively resistant to gastroenteritis (Alexander, 1948 ;Ross and Dawes, 1954;Hinton and MacGregor, 1958) ; various explanations suggested for this include passive transfer of antibodies to Esclzerichia coli in colostrum (Sussman, 1961 ;Michael, Ringenback and Hottenstein, 197 l), contamination of artificial feeds during preparation (Neter, 1959) and the nature of the intestinal environment (Ross and Dawes, 1954). The continuing occurrence of enteropathogenic E. coli infections in infants, together with the introduction of " humanised '' milks and the popularity of supplementing breast feeding with cows' milk preparations during the 1st week of life, has led us to re-examine this problem.…”

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confidence: 99%