Enterohaemorrhagic Escherichia coli in human medicine

Karch, Helge; Tarr, Phillip I.; Bielaszewska, Martina

doi:10.1016/j.ijmm.2005.06.009

Cited by 590 publications

(472 citation statements)

References 138 publications

(195 reference statements)

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“…This foodborne enteric pathogen is acquired from contaminated foodstuffs and non-chlorinated drinking water (Karch et al, 2005;Maki, 2006;Rangel et al, 2005). In the severest cases of infection (~10-15 % of cases), the haemolytic uraemic syndrome develops as a systemic complication, leading to patient hospitalization, kidney failure and death (Serna & Boedeker, 2008;Tarr et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Probiotics prevent enterohaemorrhagic Escherichia coli O157 : H7-mediated inhibition of interferon-γ-induced tyrosine phosphorylation of STAT-1

et al. 2009

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Enterohaemorrhagic Escherichia coli (EHEC) O157 : H7 inhibits interferon (IFN)-c-stimulated tyrosine phosphorylation of signal transducer and activator of transcription (STAT)-1 in epithelial cells. We determined the effects of probiotics on EHEC-mediated disruption of IFN-c-stimulated STAT-1 activation in epithelial cell lines. Confluent Intestine 407, HEp-2 and Caco-2 epithelial cells were pre-treated (3 h) with either probiotics or surface-layer proteins derived from Lactobacillus helveticus R0052 prior to infection with EHEC O157 : H7 strain CL56 (m.o.i. 100 : 1, 6 h, 37 6C in 5 % CO 2 ). Subsequently, cells were washed and stimulated with human recombinant IFN-c (50 ng ml "1 , 0.5 h, 37 6C) followed by whole-cell protein extraction and immunoblotting for tyrosine-phosphorylated STAT-1. Relative to uninfected cells, STAT-1-activation was reduced after EHEC O157 : H7 infection. Pre-incubation with the probiotic L. helveticus R0052 followed by EHEC infection abrogated pathogen-mediated disruption of IFNc-STAT-1 signalling. As determined using Transwell inserts, probiotic-mediated protection was independent of epithelial cell contact. In contrast, pre-incubation with boiled L. helveticus R0052, an equal concentration of viable Lactobacillus rhamnosus R0011, or surface-layer proteins (0.14 mg ml "1 ) did not restore STAT-1 signalling in EHEC-infected cells. The viable probiotic agent L. helveticus R0052 prevented EHEC O157 : H7-mediated subversion of epithelial cell signal transduction responses. INTRODUCTIONOutbreaks of enterohaemorrhagic Escherichia coli (EHEC) serotype O157 : H7 infection occur frequently across the developed world (Serna & Boedeker, 2008). This foodborne enteric pathogen is acquired from contaminated foodstuffs and non-chlorinated drinking water (Karch et al., 2005;Maki, 2006;Rangel et al., 2005). In the severest cases of infection (~10-15 % of cases), the haemolytic uraemic syndrome develops as a systemic complication, leading to patient hospitalization, kidney failure and death (Serna & Boedeker, 2008;Tarr et al., 2005). Renal disease results from bacterial cytotoxin [Shiga-like toxin (Stx)-1 and -2] production in the gut, release into the systemic circulation and damage to vascular endothelial cells in the renal glomerulus (Blackall & Marques, 2004). EHEC pathogenesis is also attributed to intimate bacterial adhesion conferred by the locus of enterocyte effacement (LEE) pathogenicity island, the activity of effector proteins secreted through a type three secretion system (T3SS), and pathogen modulation of host cell signal transduction cascades (Bhavsar et al., 2007;Kaper et al., 2004).EHEC disease pathogenesis includes subversion of epithelial cell signal transduction cascades involved in host innate immune responses to infection (Bhavsar et al., 2007). For instance, EHEC inhibits interferon (IFN)-c-stimulated tyrosine phosphorylation of signal transducer and activator of transcription-1 (STAT-1) in multiple cell lines (Ceponis et al., 2003), which is mediated by a bacterially encoded fac...

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Section: Introductionmentioning

confidence: 99%

Probiotics prevent enterohaemorrhagic Escherichia coli O157 : H7-mediated inhibition of interferon-γ-induced tyrosine phosphorylation of STAT-1

et al. 2009

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“…Human infections have been associated with undercooked meat, unpasteurized milk or contamined fruit, vegetables or water (2,5,19,20) and cattle are considered the main reservoir of these strains.…”

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confidence: 99%

Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA)

Bustamante¹,

Lucchesi²,

Parma³

2009

Braz. J. Microbiol.

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The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates studied, the panel of VNTR used made it possible to perform a first approach of the high genetic diversity of native strains of O157:H7 by MLVA.

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“…1, 2 For example, verotoxin-producing Escherichia coli (VT EC) produce Shiga-like toxin, a main source of foodborne illness. 2,3 VT EC are oftentimes found in contaminated water, meat, dairy products and juice. 4 When infecting humans, they example, within the European Union the permitted E. coli concentration of pasteurized milk is 100 CFU/ml 10 and that of milk for cheese production up to 10 4 CFU/ml.…”

Section: Introductionmentioning

confidence: 99%

A versatile-deployable bacterial detection system for food and environmental safety based on LabTube-automated DNA purification, LabReader-integrated amplification, readout and analysis

Hoehl

Bocholt

Kloke³

et al. 2014

Analyst

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Contamination of foods is a public health hazard that episodically causes thousands of deaths and sickens millions worldwide. T o ensure food safety and quality, rapid, low-cost and easy-to-use detection methods are desirable. Here, the LabSystem is introduced for integrated, automated DNA purification, 10 amplification and detection. It consists of a disposable, centrifugally -driven DNA purification platform (LabT ube) and the subsequent amplification and detection in a low-cost UV/vis-reader (LabReader). For demonstration of the LabSystem in the context of food safety, purification of Escherichia coli (nonpathogenic E. coli and pathogenic verotoxin-producing E. coli (VT EC)) in water and milk, and the product-spoiler Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice was integrated and 15 optimized in the LabT ube. Inside the LabReader, the purified DNA was amplified, readout and analyzed using both qualitative isothermal loop-mediated DNA amplification (LAMP) and quantitative real-time PCR. For the LAMP -LabSystem, the combined detection limits for purification and amplification of externally lysed VT EC and A. acidoterrestris is 10 2 -10 3 cell-equivalents. In the PCR-LabSystem for E. coli cells, the quantification limit is 10 2 cell-equivalents including LabT ube-integrated lysis. T he 20 demonstrated LabSystem only requires a laboratory centrifuge (to operate the disposable, fully closed LabT ube) and the low-cost LabReader for DNA amplification, readout and analysis. Compared with commercial DNA amplification devices, the LabReader improves sensitivity and specificity by the simultaneous readout of four wavelengths and the continuous readout during temperature cycling. T he use of a detachable eluate tube as an interface affords semi-automation of the LabSystem, which does not 25 require specialized training. It reduces hands-on time from about 50 to 3 min with only two handling steps: sample input and transfer of the detachable detection tube.

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Enterohaemorrhagic Escherichia coli in human medicine

Cited by 590 publications

References 138 publications

Probiotics prevent enterohaemorrhagic Escherichia coli O157 : H7-mediated inhibition of interferon-γ-induced tyrosine phosphorylation of STAT-1

Probiotics prevent enterohaemorrhagic Escherichia coli O157 : H7-mediated inhibition of interferon-γ-induced tyrosine phosphorylation of STAT-1

Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA)

A versatile-deployable bacterial detection system for food and environmental safety based on LabTube-automated DNA purification, LabReader-integrated amplification, readout and analysis

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