Enterohepatic Helicobacter spp. in cats with non-haematopoietic intestinal carcinoma: a survey of 55 cases

Abstract

Several enterohepatic Helicobacter spp. (EHS) have been isolated from cats. Despite the reported association between EHS infection and intestinal neoplasia in other species, this association has not been explored in cats. In this study, 55 non-haematopoietic feline intestinal carcinoma cases were histopathologically evaluated. In contrast with prior reports, large intestinal (LI) carcinoma was observed with greater frequency (61 %) relative to small intestinal (SI) carcinoma (35 %). There was a significant association between intestinal location and animal gender. Of males examined, 83 % had LI carcinoma, while no such trend was observed in females. Previously described associations between Siamese breed and intestinal carcinoma could not be definitively confirmed, although the Siamese breed may be predisposed to SI carcinoma location. Of all carcinomas examined in this study, 62 % were classified as adenocarcinoma, although mucinous adenocarcinoma (28 %) and solid carcinoma (11 %) were also identified. Tumours were all moderately or poorly differentiated. When considered by intestinal location and histopathologic classification, LI adenocarcinoma was associated with significantly advanced mean age (13 years) when compared to SI adenocarcinoma and LI mucinous adenocarcinoma (mean, 9 years in both cases), which were also frequently encountered. To determine whether EHS might play a role in feline intestinal neoplasia, Helicobacter genus-and species-specific fluorescence in situ hybridization was performed. Of these carcinoma cases, 56 % were positive for Helicobacter spp. and one or more speciesspecific assay for Helicobacter bilis, Helicobacter canis or Helicobacter marmotae. The presence of EHS was significantly associated with both LI location (68 %) and mucinous adenocarcinoma (92 %). These findings suggest a role for intestinal bacteria in non-haematopoietic feline intestinal neoplasia.

Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats

“…46 In a recent study, FISH showed that the presence of enterohepatic helicobacter was significantly associated with poorly differentiated large intestinal adenocarcinoma in cats. 48 Our results showed no significant difference in Helicobacter spp. bacterial counts in GI biopsies of cats with small cell GI LSA compared to GI biopsies of cats with IBD.…”

“…induces host responses influencing risk of oncogenesis, and promotes chronic gut inflammation in humans and mice 46. In a recent study, FISH showed that the presence of enterohepatic helicobacter was significantly associated with poorly differentiated large intestinal adenocarcinoma in cats 48. Our results showed no significant difference in Helicobacter spp.…”

“…This probe array was selected to identify specific bacterial groups and individual bacterial species previously shown to be relevant in the pathogenesis of intestinal cancers in humans, rodents, and dogs. 9,14,19,22,23,27,[35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] Tissue sections were bathed in 30μL of DNA-probe-hybridization buffer mix in a hybridization chamber maintained at 54 C overnight (12 hours). Buffer 1 (20mM Tris, 0.9M NaCl, 0.1% SDS, 20% Formamide, 10% Dextran sulfate) was used for the hybridization of Eub338, Erec482, Bacto1080, Fuso0664, Ebac1790 and Faecali698.…”

Complete Genome Sequencing and Comparative Genomic Analysis of Helicobacter Apodemus Isolated From the Wild Korean Striped Field Mouse (Apodemus agrarius) for Potential Pathogenicity

“…To date, more than 37 Helicobacter species have been identified in addition to H. pylori (Péré-Védrenne et al, 2017 ). Furthermore, non- H. pylori Helicobacters (NHPH) have been shown to infect both humans and animals, and NHPH infections are associated with intestinal carcinoma, and mucinous adenocarcinoma (Swennes et al, 2016 ). Despite the demonstrated association between NHPH and disease, most studies to date have investigated H. pylori in humans; thus, it is necessary to characterize NHPH and elucidate its role in the GI tract of wild rodents which are potential Helicobacter carriers (Taylor et al, 2007 ; Mladenova-Hristova et al, 2017 ).…”

Other Helicobacters, gastric and gut microbiota

“…in cats with nonhematopoietic intestinal carcinoma (55 cases) was shown to be as high as 56%, and one or more speciesspecific assays were positive for Helicobacter bilis, Helicobacter canis, or Helicobacter marmotae. The presence of NHPH was significantly associated with large intestinal carcinoma (68%) and mucinous adenocarcinoma (92%) 11. Sabry et al evaluated 149 animals and 10 human fecal samples for the presence of Helicobacter sp.…”