1964
DOI: 10.3181/00379727-115-29071
|View full text |Cite
|
Sign up to set email alerts
|

Enterovirus Typing by Complement Fixation

Abstract: Complement fixation has been successfully employed in identifying unknown enteroviruses by their reactions with poliomyelitis ( 1-3 ) , ECHO Types 1-14(4-6), and Coxsackie Group B, Type l ( 5 ) antisera. This report extends the experience to identifications with specific monkey antisera of ECHO Types 15-25, Coxsackie Group B, Types 2-5, and Group A, Type 9. One hundred and fortyeight freshly isolated enterovirus samples grown in monkey kidney tissue culture were classified and the results correlated with neutr… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

1967
1967
1981
1981

Publication Types

Select...
3
2

Relationship

0
5

Authors

Journals

citations
Cited by 5 publications
(2 citation statements)
references
References 1 publication
0
2
0
Order By: Relevance
“…Immediately before use, the plates were washed five times with phosphate-buffered saline (pH 7.4) containing 0.05% Tween 20, and a portion of antigen, tissue culture fluid, or clinical specimen was added to the wells in such a way that the test specimen was reacted in two wells coated with an individual monkey anticoxsackievirus antiserum and in two wells coated with the normal monkey serum (24). Complement fixation antigens were tested at a concentration of 10-2 complement fixation units as defined by Gnesh et al (8). Clinical specimens and tissue culture fluids were diluted at least 1:10 before testing.…”
Section: Methodsmentioning
confidence: 99%
“…Immediately before use, the plates were washed five times with phosphate-buffered saline (pH 7.4) containing 0.05% Tween 20, and a portion of antigen, tissue culture fluid, or clinical specimen was added to the wells in such a way that the test specimen was reacted in two wells coated with an individual monkey anticoxsackievirus antiserum and in two wells coated with the normal monkey serum (24). Complement fixation antigens were tested at a concentration of 10-2 complement fixation units as defined by Gnesh et al (8). Clinical specimens and tissue culture fluids were diluted at least 1:10 before testing.…”
Section: Methodsmentioning
confidence: 99%
“…[9,111 . While other methods of identifying Coxsackie viruses in tissue cultures such as immunofluorescence [16] , complement fixation (CF) [5] and hemagglutination inhibition [14] have been described, these methods have not attained widespread usage in clinical diagnostic laboratories.…”
Section: Introductionmentioning
confidence: 99%