Entomopathogen Metarhizium anisopliae promotes the early development of peanut root

Liu, Shaofang; Wang, Guangjun; Nong, Xiangqun; Liu, Bin; Wang, Miaomiao; Li, Shanlin; Cao, Guangchun; Zhang, Zehua

doi:10.17221/49/2016-pps

Cited by 23 publications

(3 citation statements)

References 25 publications

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“… 13 – 16 . Treating crop seed with B. bassiana and M. anisopliae resulted in successful endophytic colonization in crop tissues and promoted crop growth, as evidenced by stem height, root length, fresh root and fresh stem weight 17 – 19 . Soil inoculation and foliar spraying of B. bassiana are also the most used application method which considerably enhanced the growth of corn seedlings 20 .…”

Section: Introductionmentioning

confidence: 99%

Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae play roles of maize (Zea mays) growth promoter

Liu

Yang

Wang

2022

Sci Rep

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Beauveria bassiana and Metarhizium anisopliae are two of the most important and widely used entomopathogenic fungi (EPFs) to control insect pests. Recent studies have revealed their function in promoting plant growth after artificial inoculation. To better assess fungal colonization and growth-promoting effects of B. bassiana and M. anisopliae on crops, maize Zea mays seedlings were treated separately with 13 B. bassiana and 73 M. anisopliae as rhizosphere fungi in a hydroponic cultural system. Plant growth indexes, including plant height, root length, fresh weight, etc., were traced recorded for 35 days to prove the growth promoting efficiency of the EPFs inoculation. Fungal recovery rate (FRR) verified that both B. bassiana and M. anisopliae could endophytically colonize in maize tissues. The recovery rates of B. bassiana in stems and leaves were 100% on the 7th day, but dropped to 11.1% in the stems and 22.2% in the leaves on the 28th day. Meanwhile, B. bassiana was not detected in the roots until the 28th day, reaching a recovery rate of 33.3%. M. anisopliae strains were isolated from the plant roots, stems and leaves throughout the tracing period with high recovery rates. The systematical colonization of B. bassiana and M. anisopliae in different tissues were further corroborated by PCR amplification of fungus-specified DNA band, which showed a higher detection sensitivity of 100% positive reaction. Fungal density comparing to the initial value in the hydroponic solution, dropped to be well below 1% on the 21st day. Thus, the two selected entomopathogenic fungal strains successfully established endophytic colonization rather than rhizospheric colonization in maize, and significantly promoted its growth in a hydroponic cultural system. Entomopathogenic fungi have great application potential in eco-agricultural fields including biopesticides and biofertilizers.

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Section: Introductionmentioning

confidence: 99%

Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae play roles of maize (Zea mays) growth promoter

Liu

Yang

Wang

2022

Sci Rep

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“…Metarhizium anisopliae was reportedly also effective in killing S. litura larvae (Sowmya et al 2017;Yang et al 2019;Gustianingtyas et al 2020) and can kill 96% of S. litura larvae (Gustianingtyas et al 2020). Furthermore, Metarhizium rileyi also effectively kills S. litura larvae (Liu et al 2017). In addition to being effective in killing the insect pests, EPF can also be integrated with entomopathogenic viruses, such as nucleopolyhedroviruses (NPV) (Souza et al 2019) and botanical insecticides (Hernandez-Trejo et al 2019) and does not harm parasitoids (Ngangambe and Mwatawala 2020).…”

Section: Introductionmentioning

confidence: 99%

New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura

et al. 2020

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Abstract. Herlinda S, Efendi RA, Suharjo R, Hasbi, Setiawan A, Elfita, Verawaty M. 2020. New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas 21: 5102-5113. This study was carried out to confirm identity of Beauveria bassiana and Metarhizium anisopliae using sequence analysis of Internal Transcribed Spacer (ITS) region as well as the two unknown isolates of EPF and to investigate their filtrate and conidial insecticidal activity against S. litura larvae. The EPF species were identified based on morphological characters and molecular analysis. Their culture filtrate and conidial bioassays were performed on S. litura larvae. The species found were Penicillium citrinum (BKbTp), Talaromyces diversus (MSwTp1), B. bassiana (BSwTd4), and M. anisopliae (MSwTp3) and have been deposited in the GenBank. All EPF species have high insecticidal activity against S. litura larvae. The highest larvae mortality caused by filtrate was found on P. citrinum (98.67%) and not significantly different from those of T. diversus (96%), but significantly different from B. bassiana (86.67%) and M anisopliae (82.67%). The highest larvae mortality caused by the conidia was also occurred to P. citrinum (98.67%) which was not significantly different from those of T. diversus (97.33%), B. bassiana (86.67%), and M. anisopliae (86.67%). The LT50 caused by T. diversus filtrate was the shortest (6.19 days) and not significantly different from those caused by P. citrinum (6.62 days). The shortest LT50 caused by conidia was found on P. citrinum (5.45 days). Therefore, the conidia and filtrate of P. citrinum, a new emerging entomopathogenic fungus, has the highest insecticidal activity. P. citrinum has the potential to be developed into commercial mycoinsecticides.

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“…Plant growth promotion has been observed for multiple species of Metarhizium spp. in tomato [33,34], maize [32,35,36,37], soybean [38], peanut [39], potato [40], cassava [41], sweet pepper [42], switchgrass and haricot beans [43].…”

Section: Introductionmentioning

confidence: 99%

In vitro Screening of Natural Metarhizium spp. Isolates for Bioinsecticidal and Biofungicidal Activity

Doolotkeldieva,

Tolonbekova,

Bobusheva

2024

JAMB

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Aims: To increase conidia production of local Metarhizium spp—strains by selecting the low-cost media to test obtained bioformulation against the sucking pests and fungal plant pathogens. Study Design: The phytophages like black bean aphid (Aphis fabae), a scale insect (Aspidiotus nerii) and spider mite (Tetranychus urticae) are economically essential pests in Kyrgyzstan. The current requirement is to use environmentally friendly protection to reduce the number of these pests. The entomopathogenic fungus from the Metarhizium genus was used to reduce harmful pests in vitro and in vivo experiments. Place and Duration of Study: Department of Plant Protection, Kyrgyz-Turkish Manas University between May 2021 and May 2023. Methodology: Natural Metarhizium spp. strains were isolated from dead insect bodies of the Lepidoptera and Apidae families. A laboratory bioassay was conducted to evaluate isolated Metarhizium spp.strains against nymphal stages and adults of sucking pests like A. fabae ((Blackfly), A.nerii (Scale insect) and T.urticae (Spider mite). The inhibitory effect of Metarhizium spp.against Fusarium oxysporum, Alternaria spp. and Rhizoctonia solani was detected using a dual culture technique. Results: The results of in vitro bioassay tests against sucking pests have revealed the LC50 and LT50 values of TLK-1 isolate. The LC50 of this isolate for A. fabae adults was 1 × 106 conidia\ mL-1; for A.nerii, it was lower - 7 × 105 conidia\ ml-1; while for adults T.urticae it was higher- 13 × 106 conidia \ ml-1. Such mortality occurred after 49.63 hours in adults of A. fabae (LT50), after 46.30 hours in A.nerii (LT50), and after 75.87 hours in deutonymph and adults of T. urticae (LT50). Three isolates, TLK-1, TLA-2 and BZТК1, have exhibited biofingicide activity in pathogen fungi like Fusarium oxysporum and Alternaria spp. Conclusion: The found isolates can be simultaneously used against pathogenic fungi, essential for developing inexpensive biological formulas based on these fungi.

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Entomopathogen Metarhizium anisopliae promotes the early development of peanut root

Cited by 23 publications

References 25 publications

Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae play roles of maize (Zea mays) growth promoter

Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae play roles of maize (Zea mays) growth promoter

New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura

In vitro Screening of Natural Metarhizium spp. Isolates for Bioinsecticidal and Biofungicidal Activity

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