Entrapment of Lactobacillus acidophilus into alginate beads for the effective treatment of cold restraint stress induced gastric ulcer

Abstract

Lactobacillus acidophilus (LAB) loaded alginate floating beads (FBs) were developed with an intent to (i) preserve their viability during manufacture and upon exposure to adverse physiological conditions existing in the stomach, (ii) achieve an increased stay of the system in the stomach for improved pharmacodynamics and to provide for their effective establishment within the gastric mucosa. In vitro characterization of developed beads was performed in terms of entrapment efficiency, buoyancy, and surface as well as cross sectional morphology and viability studies of LAB in a gastric environment. The developed system was evaluated and was found to be significantly better in an experimental model of cold restraint stress (CRS) induced gastric ulcer model in terms of ulcer index, hemorrhagic streak length, histopathological and biochemical markers and their cross talk with reactive oxygen/nitrogen species. The present study emphasizes the advantages and future potential of probiotic loaded FBs in gastric disorders.

Role of Lactobacillus acidophilus loaded floating beads in chronic fatigue syndrome: behavioral and biochemical evidences

“…We evaluated the effect of administering LAB to rats with CFS and observed a significant improvement which was further enhanced by incorporating LAB into FBs. Latter improves the stay of LAB with in stomach to more than 10 h 31,32,40 while in normal routine orally administered material passes out of the stomach within 2–4 h. Further to this, incorporation within FBs improves the viability of LAB in the gastric fluid as shown in Table 1.…”

“…Measured amount (20 mg LAB) of the probiotic was suspended in water. Details of the procedure are reported earlier 31,39,40 . The beads were prepared aseptically using sterile solutions, vehicle and container.…”

“…We have also reported on the entrapment of LAB within alginate beads to prolong their stay in GIT (for better establishment in gut mucosa), maintaining the viability especially in acidic conditions of the stomach. The developed beads also showed an enhanced performance in the gastric ulcer model 31,32 …”

Tocotrienol Attenuates Stress-Induced Gastric Lesions via Activation of Prostaglandin and Upregulation of COX-1 mRNA

“…The pathological basis for the development of stress-induced gastric lesions has been postulated to be multifactorial. These include changes in the gastric acid secretion [2], reduction of gastric mucosal blood flow [3], inhibition of gastric mucus and bicarbonate secretion [4], and inhibition of gastric mucosal prostaglandin synthesis [5]. …”

Novel Drug Delivery Systems for Modulation of Gastrointestinal Transit Time

“…They evaluated the efficacy of the approach in experimental model of cold restraint stress induced gastric ulcer in terms of ulcer index; hemorrhagic streak length; mucus content and histopathological examinations. According to the authors, the developed formulations not only efficiently protected the entrapped probiotic cells, but also effectively delivered and retained viable bacteria in the stomach [Singh et al, 2012]. The prolonged and continuous release of the probiotic in the gastric mucosa allowed them for an ensured therapeutic efficacy of the developed floating system against ulcers post-induction, thus suggesting an efficient line of treatment.…”