In this research, the three-dimensional nanofluid thin-film flow of Casson fluid over an inclined steady rotating plane is examined. A thermal radiated nanofluid thin film flow is considered with suction/injection effects. With the help of similarity variables, the partial differential equations (PDEs) are converted into a system of ordinary differential equations (ODEs). The obtained ODEs are solved by the homotopy analysis method (HAM) with the association of MATHEMATICA software. The boundary-layer over an inclined steady rotating plane is plotted and explored in detail for the velocity, temperature, and concentration profiles. Also, the surface rate of heat transfer and shear stress are described in detail. The impact of numerous embedded parameters, such as the Schmidt number, Brownian motion parameter, thermophoretic parameter, and Casson parameter (Sc, Nb, Nt, γ), etc., were examined on the velocity, temperature, and concentration profiles, respectively. The essential terms of the Nusselt number and Sherwood number were also examined numerically and physically for the temperature and concentration profiles. It was observed that the radiation source improves the energy transport to enhance the flow motion. The smaller values of the Prandtl number, Pr, augmented the thermal boundary-layer and decreased the flow field. The increasing values of the rotation parameter decreased the thermal boundary layer thickness. These outputs are examined physically and numerically and are also discussed.