Entropy production rate is maximized in non-contractile actomyosin

Seara, Daniel S.; Yadav, Vikrant; Linsmeier, Ian; Tabatabai, A. Pasha; Oakes, Patrick W.; Tabei, S. M. Ali; Banerjee, Shiladitya; Murrell, Michael P.

doi:10.1038/s41467-018-07413-5

Cited by 63 publications

(57 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once the myosin filaments reach the plus end of an actin filament, they could contribute to the condensation of actin filaments into clusters by catching and pulling adjacent actin filaments, leading to the formation of contractile zones in more-crowded regions of the actin filament network ( 3 , 51 , 52 ). The effect of myosin II filaments cross-linking multiple actin filaments on network contractility and entropy production was also reported by the Murrell group ( 42 ).…”

Section: Discussionsupporting

confidence: 62%

See 1 more Smart Citation

Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy

Mosby

Hundt

Young

et al. 2020

Biophysical Journal

View full text Add to dashboard Cite

The plasma membrane and the underlying cytoskeletal cortex constitute active platforms for a variety of cellular processes. Recent work has shown that the remodeling acto-myosin network modifies local membrane organization, but the molecular details are only partly understood because of difficulties with experimentally accessing the relevant time and length scales. Here, we use interferometric scattering microscopy to investigate a minimal acto-myosin network linked to a supported lipid bilayer membrane. Using the magnitude of the interferometric contrast, which is proportional to molecular mass, and fast acquisition rates, we detect and image individual membrane-attached actin filaments diffusing within the acto-myosin network and follow individual myosin II filament dynamics. We quantify myosin II filament dwell times and processivity as functions of ATP concentration, providing experimental evidence for the predicted ensemble behavior of myosin head domains. Our results show how decreasing ATP concentrations lead to both increasing dwell times of individual myosin II filaments and a global change from a remodeling to a contractile state of the acto-myosin network.

show abstract

Section: Discussionsupporting

confidence: 62%

“…However, the observed increase of

with decreasing ATP concentrations could be due to the binding of multiple myosin heads simultaneously to an actin filament. This rotational flexibility is likely to allow myosin filaments to connect multiple actin filaments ( 40 ), which would be important to generate network contraction ( 41 , 42 ).…”

Section: Resultsmentioning

confidence: 99%

Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy

Mosby

Hundt

Young

et al. 2020

Biophysical Journal

View full text Add to dashboard Cite

show abstract

“…The changes of the actin filaments organization were quantified by an order parameter , where the image was segmented to the number of overlapping blocks, then the local actin orientation (director) was calculated for each block, and is the angle difference between the directors of the central block and surrounding blocks (Code used to measure the nematic order parameter is available at: https://github.com/OakesLab/FFT_Alignment .) 70 , 71 . ranges from zero to one which is showing randomly oriented directors and parallel directors respectively.…”

Section: Resultsmentioning

confidence: 99%

Microfluidic investigation of the effect of graphene oxide on mechanical properties of cell and actin cytoskeleton networks: experimental and theoretical approaches

Ghorbani

Soleymani

Hashemzadeh

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Biomechanical and morphological analysis of the cells is a novel approach for monitoring the environmental features, drugs, and toxic compounds’ effects on cells. Graphene oxide (GO) has a broad range of medical applications such as tissue engineering and drug delivery. However, the effects of GO nanosheets on biological systems have not been completely understood. In this study, we focused on the biophysical characteristics of cells and their changes resulting from the effect of GO nanosheets. The biophysical properties of the cell population were characterized as follows: cell stiffness was calculated by atomic force microscopy, cell motility and invasive properties were characterized in the microfluidic chip in which the cells are able to visualize cell migration at a single-cell level. Intracellular actin was stained to establish a quantitative picture of the intracellular cytoskeleton. In addition, to understand the molecular interaction of GO nanosheets and actin filaments, coarse-grained (CG) molecular dynamics (MD) simulations were carried out. Our results showed that GO nanosheets can reduce cell stiffness in MCF7 cells and MDA-MB-231 cell lines and highly inhibited cell migration (39.2%) in MCF-7 and (38.6%) in MDA-MB-231 cell lines through the GO nanosheets-mediated disruption of the intracellular cytoskeleton. In the presence of GO nanosheets, the cell migration of both cell lines, as well as the cell stiffness, significantly decreased. Moreover, after GO nanosheets treatment, the cell actin network dramatically changed. The experimental and theoretical approaches established a quantitative picture of changes in these networks. Our results showed the reduction of the order parameter in actin filaments was 23% in the MCF7 cell line and 20.4% in the MDA-MB-231 cell line. The theoretical studies also showed that the GO nanosheet–actin filaments have stable interaction during MD simulation. Moreover, the 2D free energy plot indicated the GO nanosheet can induce conformational changes in actin filaments. Our findings showed that the GO nanosheets can increase the distance of actin-actin subunits from 3.22 to 3.5 nm and in addition disrupt native contacts between two subunits which lead to separate actin subunits from each other in actin filaments. In this study, the biomechanical characteristics were used to explain the effect of GO nanosheets on cells which presents a novel view of how GO nanosheets can affect the biological properties of cells without cell death. These findings have the potential to be applied in different biomedical applications.

show abstract

“…For example, bulk order parameters in complex reactions can switch from exhibiting incoherent, disordered behavior to stable static patterns 10,11 or traveling waves of excitation 12,13 that break time reversal symmetry in both time and space simply by altering the strength of the microscopic driving force. Recent advances in stochastic thermodynamics have highlighted entropy production as a quantity to measure a system's distance from equilibrium [14][15][16][17][18][19] . While much work has been done investigating the critical behavior of entropy production at continuous and discontinuous phase transitions [20][21][22][23][24][25][26][27][28] , dynamical phase transitions in spatially extended systems have only recently been investigated, and to date no non-analytic behavior in the entropy production has been observed 29,30 .…”

mentioning

confidence: 99%

Irreversibility in dynamical phases and transitions

2021

Self Cite

View full text Add to dashboard Cite

Living and non-living active matter consumes energy at the microscopic scale to drive emergent, macroscopic behavior including traveling waves and coherent oscillations. Recent work has characterized non-equilibrium systems by their total energy dissipation, but little has been said about how dissipation manifests in distinct spatiotemporal patterns. We introduce a measure of irreversibility we term the entropy production factor to quantify how time reversal symmetry is broken in field theories across scales. We use this scalar, dimensionless function to characterize a dynamical phase transition in simulations of the Brusselator, a prototypical biochemically motivated non-linear oscillator. We measure the total energetic cost of establishing synchronized biochemical oscillations while simultaneously quantifying the distribution of irreversibility across spatiotemporal frequencies.

show abstract

Entropy production rate is maximized in non-contractile actomyosin

Cited by 63 publications

References 62 publications

Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy

Myosin II Filament Dynamics in Actin Networks Revealed with Interferometric Scattering Microscopy

Microfluidic investigation of the effect of graphene oxide on mechanical properties of cell and actin cytoskeleton networks: experimental and theoretical approaches

Irreversibility in dynamical phases and transitions

Contact Info

Product

Resources

About