Entry of Alphaherpesviruses Mediated by Poliovirus Receptor-Related Protein 1 and Poliovirus Receptor

Geraghty, Robert J.; Krummenacher, Claude; Cohen, Gary H.; Eisenberg, Roselyn J.; Spear, Patricia G.

doi:10.1126/science.280.5369.1618

Cited by 847 publications

(846 citation statements)

References 18 publications

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“…For many HSV-1 types, HveA and HveC seem to be the predominant receptor, whereas entry of most HSV-2 involves HveB. 14,16,18 We have previously demonstrated that HSV-2-based recombinant vectors can infect primary human ALL with high efficiency. 3 In accordance with high level expression of all three Hve receptors, we have now extended this observation to HSV-1-derived amplicon vectors.…”

Section: Discussionmentioning

confidence: 99%

“…With regard to primary human cells, we focused our analysis on ALL, the single most common childhood malignancy. The entry of HSV into mammalian cells involves HSV envelope glycoproteins B and/or C. [12][13][14][15] Additional receptors are critical for viral entry into target cells termed herpes virus entry mediators (Hve). HveA is a member of the tumor necrosis factor receptor family, 16,17 whereas HveB and HveC belong to proteins of the immunoglobulin superfamily.…”

Section: Introductionmentioning

confidence: 99%

“…HveA is a member of the tumor necrosis factor receptor family, 16,17 whereas HveB and HveC belong to proteins of the immunoglobulin superfamily. 14,18 Thus, in addition to gene transfer rates, we examined expression of all three HSV receptor genes HveA, HveB and HveC 14,[16][17][18] following infection of primary ALL blasts with HSV-1 amplicon vectors.…”

Section: Introductionmentioning

confidence: 99%

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Herpes simplex virus type-1 amplicon vectors for vaccine generation in acute lymphoblastic leukemia

et al. 2005

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For leukemia vaccine generation, high-efficiency gene transfer is required to express immunomodulatory molecules that stimulate potent antileukemic immune responses. In this context, herpes simplex virus type-1 (HSV-1)-derived vectors have proven to be a promising tool for genetic modification of lymphoblastic leukemia cells. Yet, vector-associated viral protein expression might inadvertently modulate vaccine efficacy facilitating both immune evasion and immune stimulation. To explore the issue of immune-stimulation versus immune-suppression in immature lymphoblastic leukemia cells, two types of HSV-1 amplicon vectors, helper virus-dependent and helper virus-free that express the immunomodulatory molecules CD70 and IL-2, were compared with regard to their vector-associated immunomodulatory potential. We first established that lymphoblastic cell lines and primary acute lymphoblastic leukemia (ALL) cells express HSV receptor genes. Lymphoblastic cell lines were transduced with high efficiency, and in primary ALL cells high gene transfer rates of 47715 and 42714% were obtained with helper virus-dependent and -free HSV-1 amplicon vectors, respectively. The efficacy of the two amplicon vectors to induce antineoplastic responses was assessed in a vaccine setting in mice with pre-existing highly malignant lymphoblastic disease. Treatment of mice with vaccine cells transgenically expressing CD70+IL2 significantly suppressed lymphoblastic cell proliferation and improved survival. Of note, when helper virus-dependent HSV-1 amplicon vectors were used for vaccine preparation, the high immunogenic potential of the vector itself, in the absence of transgenic CD70+IL2 expression, seemed to be sufficient to mediate protection comparable to the antineoplastic response achieved by expression of immunomodulatory molecules. Thus for vaccine generation in B lymphoblastic leukemia, the immunogenic potential of HSV-1 helper virus-dependent amplicon vectors does provide additional benefit to the high transduction efficiency of HSV-1-derived vectors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Herpes simplex virus type-1 amplicon vectors for vaccine generation in acute lymphoblastic leukemia

et al. 2005

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“…7 HveA is a member of the TNF receptor family 8 and HveC has been identified as nectin-1a. 7,9 Flow cytometry and indirect immunocytochemistry demonstrated that these receptors were present on the surface of both human preadipocytes and mature adipocytes following differentiation in vitro (Figure 1). By flow cytometry, 2777% of cultured preadipocytes ( Figure 1a) expressed HveA, while 9075% expressed HveC (Figure 1f).…”

Section: Presence Of Hsv Entry Receptors On Adipose Cellsmentioning

confidence: 99%

HSV vector-mediated transduction and GDNF secretion from adipose cells

et al. 2004

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“…23 To initiate infection, HSV attaches to cell surface glycosaminoglycans such as heparan sulfate and dermatan sulfate, [29][30][31][32][33][34][35] which stabilize the virus such that is can interact with secondary protein receptors required for entry into host cells. [36][37][38] Thus, one possibility to account for the loss of susceptibility to HSV infection is that maturation leads to a decrease in the expression of cell surface molecules required for efficient infection.…”

Section: Introductionmentioning

confidence: 99%

Efficient infection of mature skeletal muscle with herpes simplex virus vectors by using dextran sulfate as a co-receptor

1999

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The use of herpes simplex virus (HSV) vectors for gene chondroitin ABC lyase, HSV infection was restored, which delivery to skeletal muscle is hampered by a maturationsuggests that virus secondary receptors were present but dependent loss of muscle fiber infectivity. Previous studies not readily accessible to the virus in the intact myofiber. of HSV type 1 (HSV-1) infection in the rodent show that Surprisingly, we also found that HSV-1 infectivity could be the loss of infectivity may be due, at least in part, to the restored in vitro and in vivo by exposing myofibers to low development of the basal lamina throughout the course of concentrations of the glycosaminoglycan analog dextran maturation, which may block the initial events in HSV infecsulfate, which appears to act as a surrogate receptor to tion. To initiate infection, HSV normally attaches to cell surstabilize the virus at the myofiber surface such that HSV face heparan sulfate, which stabilizes the virus such that can engage additional receptors. This demonstration that it can interact with secondary protein receptors required the basal lamina is not an absolute block to HSV-1 infecfor entry into host cells. In this study, we demonstrate that tion is remarkable because it allows for the nondestructive heparan sulfate biosynthesis is downregulated during skeltargeting of HSV-1 to mature myofibers and greatly etal muscle maturation. When myofibers were treated with expands the usefulness of HSV as a gene therapy vector a variety of enzymes, including collagenase type IV or for the treatment of inherited and acquired diseases.

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Entry of Alphaherpesviruses Mediated by Poliovirus Receptor-Related Protein 1 and Poliovirus Receptor

Cited by 847 publications

References 18 publications

Herpes simplex virus type-1 amplicon vectors for vaccine generation in acute lymphoblastic leukemia

Herpes simplex virus type-1 amplicon vectors for vaccine generation in acute lymphoblastic leukemia

HSV vector-mediated transduction and GDNF secretion from adipose cells

Efficient infection of mature skeletal muscle with herpes simplex virus vectors by using dextran sulfate as a co-receptor

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