Enumeration of bacteria in pure cultures and in foods using a commercial flow cytometer

Development of a Flow Cytometric Method To Analyze Subpopulations of Bacteria in Probiotic Products and Dairy Starters

“…FCM has been used before for bacteria in milk, but it has been used mostly for pathogens and for bacteriological quality control (10,13,20,24,29,34). FCM can also be used to measure fermentation starter bacteria.…”

“…A recently described approach for specific detection is the use of immunomagnetic separation and FCM, which was used for detection of Escherichia coli O157:H7 (29). For direct enumeration in milk, staining with ethidium bromide and mithramycin was tried; however, the sensitivity was too low to detect 6 ϫ 10 7 CFU of added E. coli per ml because of high background signals (24). The sensitivity was increased by a sampleclearing procedure using Promega's milk-clearing reagents, which was used for immunofluorescence detection of Salmonella enterica serovar Typhimurium (20).…”

Improved detection of bacteria by flow cytometry using a combination of antibody and viability markers

“…Medical applications include bacterial responses to antibiotics (Gant et al 1993 ;Mason et al 1995), analysis of blood (Mansour et al 1985) and other clinical samples (Humphreys et al 1994 ;van der Waaij et al 1994). Interest in food analysis has centred around pathogenic bacteria and spoilage organisms (Donnelly and Baigent 1986 ;Patchett et al 1991 ;Laplace-Builhé et al 1993).…”

New Insights into Cell Encapsulation and the Role of Proteins During Flow Cytometry

“…Numerous technologies, inclusive of FACS have been developed to accelerate data acquisition compared to traditional culture methods. In the food industry, techniques utilizing antibodies for cell labelling prior to FACS analysis have been developed for Salmonella enterica serovar Typhimurium and Listeria monocytogenes in milk and other dairy products (Patchett et al, 1991). Moreover, Doherty et al, (2010) demonstrated that FACS, coupled with fluorescent techniques, can be successfully applied for the assessment of cell viability in seven different protein matrices ranging in structural complexity.…”