Enumeration of circulating tumor cells and investigation of cellular responses using aptamer-immobilized AlGaN/GaN high electron mobility transistor sensor array

Pulikkathodi, Anil Kumar; Sarangadharan, Indu; Hsu, Cheng-Wei; Chen, Yi Hong; Hung, Lien Yu; Lee, Geng Yen; Chyi, Jen Inn; Lee, Gwo‐Bin; Wang, Yu Lin

doi:10.1016/j.snb.2017.10.127

Cited by 31 publications

(17 citation statements)

References 30 publications

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“…Nevertheless, the absolute values of the ∆V T shift remained approximately the same (Figure 8). A similar influence of the change in matrix on the signal direction was already observed by Pulikkathodi et al [53], who obtained an opposite response of circulating tumour cell detection in PBS and cell culture mediums. The author and his co-authors observed that this influence is due to divalent cation (Ca 2+ and Mg 2+ ) presence in the culture cell medium.…”

Section: Resultssupporting

confidence: 81%

Development of an ImmunoFET for Analysis of Tumour Necrosis Factor-α in Artificial Saliva: Application for Heart Failure Monitoring

et al. 2021

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Assessing tumour necrosis factor-α (TNF-α) levels in the human body has become an essential tool to recognize heart failure (HF). In this work, label-free, rapid, easy to use ImmunoFET based on an ion-sensitive field effect transistor (ISFET) was developed for the detection of TNF-α protein. Monoclonal anti-TNF-α antibodies (anti-TNF-α mAb) were immobilized on an ISFET gate made of silicon nitride (Si3N4) after salinization with 11-(triethoxysilyl) undecanal (TESUD). The obtained ISFET functionalized with the mAbs (ImmunoFET) was used to detect TNF-α protein in both phosphate buffer saline (PBS) and artificial saliva (AS). The change in the threshold voltage of the gate (∆VT) showed approximately linear dependency on the concentration of the antigens in the range 5–20 pg/mL for both matrixes. The cross-selectivity study showed that the developed ImmunoFET demonstrated to be selective towards TNF-α, when compared to other HF biomarkers such as N-terminal pro-brain natriuretic peptide (NT-proBNP), interleukin-10 (IL-10), and cortisol, even if further experiments have to be carried out for decreasing possible unspecific absorption phenomena. To the best of our knowledge, this is the first ImmunoFET that has been developed based on Si3N4 for TNF-α detection in AS by electrical measurement.

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Section: Resultssupporting

confidence: 81%

Development of an ImmunoFET for Analysis of Tumour Necrosis Factor-α in Artificial Saliva: Application for Heart Failure Monitoring

et al. 2021

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“…The use of gDNA allows for the extraction of enormous amounts of data from a small number of cells without the need for genome amplification. In another study, Pulikkathodi et al developed an AlGaN/GaN high-electron-mobility (HEMT) biosensor array for the detection and isolation of CTCs [165]. Furthermore, these chips are mounted on a thermoscurable polymeric substrate.…”

Section: Biological Interaction-based Isolationmentioning

confidence: 99%

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

et al. 2022

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CTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood samples has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, including immunomagnets, macroscale filters, centrifuges, dielectrophoresis, and immunological approaches. These procedures, on the other hand, are time-consuming and necessitate multiple high-level operational protocols. In addition, considering their low efficiency and throughput, the processes of capturing and isolating CTCs face tremendous challenges. Meanwhile, recent advances in microfluidic devices promise unprecedented advantages for capturing and isolating CTCs with greater efficiency, sensitivity, selectivity and accuracy. In this regard, this review article focuses primarily on the various fabrication methodologies involved in microfluidic devices and techniques specifically used to capture and isolate CTCs using various physical and biological methods as well as their conceptual ideas, advantages and disadvantages.

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“…Meanwhile, compound semiconductor-based AlGaN/GaN high electron mobility transistors (HEMTs) exhibit low on-resistance and rapid switching speeds owing to the high-mobility two-dimensional electron gas induced by the discontinuity of the conduction band in the AlGaN/GaN heterostructure and its piezoelectric polarization [10]. In addition, AlGaN/GaN HEMT-based sensors have shown potential for application for the detection of DNA, antigens, glucose, cellular responses, and gas; further, these sensors can be monolithically integrated with signal processing and amplification circuits or radio frequency (RF) signal transmission circuits, which are convenient for reading data and receiving remote signals [11][12][13][14][15][16][17][18]. Nevertheless, the leakage current of AlGaN/GaN HEMTs due to the trap-assisted tunneling and surface state is crucial.…”

Section: Introductionmentioning

confidence: 99%

High-Sensitivity pH Sensor Based on Coplanar Gate AlGaN/GaN Metal-Oxide-Semiconductor High Electron Mobility Transistor

Cho

2021

Chemosensors

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The sensitivity of conventional ion-sensitive field-effect transistors is limited to the Nernst limit (59.14 mV/pH). In this study, we developed a pH sensor platform based on a coplanar gate AlGaN/GaN metal-oxide-semiconductor (MOS) high electron mobility transistor (HEMT) using the resistive coupling effect to overcome the Nernst limit. For resistive coupling, a coplanar gate comprising a control gate (CG) and a sensing gate (SG) was designed. We investigated the amplification of the pH sensitivity with the change in the magnitude of a resistance connected in series to each CG and SG via Silvaco TCAD simulations. In addition, a disposable extended gate was applied as a cost-effective sensor platform that helped prevent damages due to direct exposure of the AlGaN/GaN MOS HEMT to chemical solutions. The pH sensor based on the coplanar gate AlGaN/GaN MOS HEMT exhibited a pH sensitivity considerably higher than the Nernst limit, dependent on the ratio of the series resistance connected to the CG and SG, as well as excellent reliability and stability with non-ideal behavior. The pH sensor developed in this study is expected to be readily integrated with wide transmission bandwidth, high temperature, and high-power electronics as a highly sensitive biosensor platform.

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Enumeration of circulating tumor cells and investigation of cellular responses using aptamer-immobilized AlGaN/GaN high electron mobility transistor sensor array

Cited by 31 publications

References 30 publications

Development of an ImmunoFET for Analysis of Tumour Necrosis Factor-α in Artificial Saliva: Application for Heart Failure Monitoring

Development of an ImmunoFET for Analysis of Tumour Necrosis Factor-α in Artificial Saliva: Application for Heart Failure Monitoring

Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

High-Sensitivity pH Sensor Based on Coplanar Gate AlGaN/GaN Metal-Oxide-Semiconductor High Electron Mobility Transistor

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