Enumeration of coliforms and Escherichia coli in frozen black tiger shrimp Penaeus monodon by conventional and rapid methods

Suwansonthichai, Sasithorn; Rengpipat, Sirirat

doi:10.1016/s0168-1605(02)00190-3

Cited by 20 publications

(9 citation statements)

References 19 publications

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“…Successful detection and/or confirmation of E. coli isolates from various water samples employing fluorogenic substartes is well documented (O'Toole & Chang, 1999;Prats et al, 2008). The results reported in this study using Fluorocult  LMX broth showed 90% and 96% accurate identification of E. coli isolates from clinical and environmental sources respectively, with no statistical significant difference (P  0.5, χ 2 ) (Table 4), these results consistent with results reported elsewhere from investigation on water and food (Suwansonthichai & Rengpipat, 2003;Abulreesh et al, 2004;Nikaeen et al, 2009). Of the 200 E. coli isolates examined in this study, 14 isolates were not identified by Fluorocult  LMX broth, four of these isolates were also not identified as E. coli by API 20E (Table 5).…”

Section: Discussionsupporting

confidence: 90%

Efficacy of Two Commercial Systems for Identification of Clinical and Environmental Escherichia coli

Abulreesh¹

2014

IJB

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The aim of this study was to test the efficacy of API 20E and Fluorocult  LMX broth in identifying a collection of 200 E. coli isolates. A total of 100 isolates originated from clinical samples (UTI) and 100 isolates from environmental water receiving faecal contamination. Randomly selected isolates that were identified by API 20E and Fluorocult  LMX broth were further identified by PCR targeting a fragment of the E. coli 16S rRNA gene. The results showed that overall 95% and 100% of the clinical and environmental isolates respectively were identified with various degrees of accuracy as E. coli by API 20E. However, only 86% of the clinical isolates and 32% of environmental isolates were identified with high level of discrimination (90% and above). Identification by Fluorocult  LMX broth successfully identified 90% and 96% of the clinical and environmental isolates respectively as E. coli. Further identification by PCR showed that 70% (n = 20) and 55% (n = 20) of the isolates that were previously identified by the two commercial systems were successfully identified by PCR. Identification of E. coli isolates of clinical and environmental origins by rapid commercial systems should be interpreted with care, PCR might be used to further confirm the result of rapid identification systems.

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Section: Discussionsupporting

confidence: 90%

Efficacy of Two Commercial Systems for Identification of Clinical and Environmental Escherichia coli

Abulreesh¹

2014

IJB

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“…CFUs were counted and expressed per gram of sample after logarithmic conversion. The coliform and E. coli assays followed Sasithorn and Sirirat [30] using 1 mL of masticated tissue liquid plated on 3 M Petrifilm no. 6414 (St. Paul, MN), and the plates were incubated at 37°C for 24 h in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Application of Highly Purified Electrolyzed Chlorine Dioxide for Tilapia Fillet Disinfection

Huang

Chung

et al. 2014

The Scientific World Journal

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This research aimed to develop an electrolysis method to generate high-concentration chlorine dioxide (ClO2) for tilapia fillet disinfection. The designed generator produced up to 3500 ppm of ClO2 at up to 99% purity. Tilapia fillets were soaked in a 400 ppm ClO2 solution for 5, 10, and 25 min. Results show that total plate counts of tilapia, respectively, decreased by 5.72 to 3.23, 2.10, and 1.09 log CFU/g. In addition, a 200 ppm ClO2 solution eliminated coliform bacteria and Escherichia coli in 5 min with shaking treatment. Furthermore, ClO2 and trihalomethanes (THMs) residuals on tilapia fillets were analyzed by GC/MS and were nondetectable (GC-MS detection limit was 0.12 ppb). The results conform to Taiwan's environmental protection regulations and act governing food sanitation.

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“…Most probable number (MPN) method was used to detect the coliforms in apple juice (Suwansonthichai and Rengpipat, 2003). In the pre-fermentation test, samples were diluted into three appropriate series, and 1.0 mL of each diluted (or non-dilute) samples was inoculated in triplicate tubes of 10 mL lauryl sulfate tryptose (LST) broth (Beijing Luqiao Biological Technology Co. Ltd., Beijing, China).…”

Section: Microbiological Enumerationmentioning

confidence: 99%

Effects of high pressure CO2 treatments on microflora, enzymes and some quality attributes of apple juice

Zhang

Wang

et al. 2011

Journal of Food Engineering

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Enumeration of coliforms and Escherichia coli in frozen black tiger shrimp Penaeus monodon by conventional and rapid methods

Cited by 20 publications

References 19 publications

Efficacy of Two Commercial Systems for Identification of Clinical and Environmental Escherichia coli

Efficacy of Two Commercial Systems for Identification of Clinical and Environmental Escherichia coli

Application of Highly Purified Electrolyzed Chlorine Dioxide for Tilapia Fillet Disinfection

Effects of high pressure CO2 treatments on microflora, enzymes and some quality attributes of apple juice

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