Enumeration of Hydrocarbon-Degrading Bacteria

Rice, Laura E.; Hemmingsen, Barbara B.

doi:10.1385/0-89603-437-2:99

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…Nevertheless it is desirable to have molecular detection methods for n‐alkane degraders as the classical quantification methods with liquid or solid mineral media are not always reliable. Especially, quantification with agar plates often leads to false‐positive results ( Rice and Hemmingsen 1997; Thurmann et al. 1999 ).…”

Section: Discussionmentioning

confidence: 99%

Distribution of alkB genes within n-alkane-degrading bacteria

Vomberg

Klinner

2000

J Appl Microbiol

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Fifty‐four bacterial strains belonging to 37 species were tested for their ability to assimilate short chain and/or medium chain liquid n‐alkanes. A gene probe derived from the alkB gene of Pseudomonas oleovorans ATCC 29347 was utilized in hybridization experiments. Results of Southern hybridization of PCR‐amplificates were compared with those of colony hybridization and dot blot hybridization. Strongest signals were received only from Gram‐negative bacteria growing solely with short n‐alkanes (C10). Hybridization results with soil isolates growing with n‐alkanes of different chain lengths suggested as well that alkB genes seem to be widespread only in solely short‐chain n‐alkane‐degrading pseudomonads. PCR products of Rhodococcus sp., Nocardioides sp., Gordona sp. and Sphingomonas sp. growing additionally or solely with medium‐chain n‐alkane as hexadecane had only few sequence identity with alkB though hybridizing with the gene probe. The derived amino acid sequence of the alkB‐amplificate of Pseudomonas aureofaciens showed high homology (95%) with AlkB from Ps. oleovorans. alkB gene disruptants were not able to grow with decane.

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Section: Discussionmentioning

confidence: 99%

Distribution of alkB genes within n-alkane-degrading bacteria

Vomberg

Klinner

2000

J Appl Microbiol

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“…The liquid medium used consisted of a mineral medium (with the following composition in g l −1 of distillate water: NaCl, 24 [24]). Petroleum from Comodoro Rivadavia provided by Shell Capsa, was sterilized in autoclave (121 • C for 15 min) and used as substrate.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation pattern of hydrocarbons from a fuel oil-type complex residue by an emulsifier-producing microbial consortium

Nievas

Commendatore

Esteves

et al. 2008

Journal of Hazardous Materials

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“…MPNs of heterotrophs able to grow aerobically were determined by adding 200 l of one-half-strength ASW mineral medium (34) Phenanthrene-degrading bacteria were enumerated by two different methods: (i) the phenanthrene overlayer method (34) and (ii) a modification of the microtiter MPN procedure of Stieber et al (38). Phenanthrene (8 g/liter) was dissolved in isopropanol and pentane (3:1), and 100 l was added to each well of a sterile microtiter plate.…”

Section: Methodsmentioning

confidence: 99%

Impact of Irradiation and Polycyclic Aromatic Hydrocarbon Spiking on Microbial Populations in Marine Sediment for Future Aging and Biodegradability Studies

Melcher

Apitz²,

Hemmingsen

2002

Appl Environ Microbiol

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Experiments were carried out to develop methods to generate well-characterized, polycyclic aromatic hydrocarbon (PAH)-spiked, aged but minimally altered sediments for fate, biodegradation, and bioavailability experiments. Changes in indigenous bacterial populations were monitored in mesocosms constructed of relatively clean San Diego Bay sediments, with and without exposure to gamma radiation, and then spiked with five different PAHs and hexadecane. While phenanthrene and chrysene degraders were present in the unspiked sediments and increased during handling, PAH spiking of nonirradiated sediments led to dramatic increases in their numbers. Phenotypic characterization of isolates able to grow on phenanthrene or chrysene placed them in several genera of marine bacteria: Vibrio, Marinobacter or Cycloclasticus, Pseudoalteromonas, Marinomonas, and Halomonas. This is the first time that marine PAH degraders have been identified as the latter two genera, expanding the diversity of marine bacteria with this ability. Even at the highest irradiation dose (10 megarads), heterotrophs and endospore formers reappeared within weeks. However, while bacteria from the unirradiated sediments had the capacity to both grow on and mineralize 14 C-labeled phenanthrene and chrysene, irradiation prevented the reappearance of PAH degraders for up to 4 months, allowing spikes to age onto the sediments, which can be used to model biodegradation in marine sediments.

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Enumeration of Hydrocarbon-Degrading Bacteria

Cited by 6 publications

References 8 publications

Distribution of alkB genes within n-alkane-degrading bacteria

Distribution of alkB genes within n-alkane-degrading bacteria

Biodegradation pattern of hydrocarbons from a fuel oil-type complex residue by an emulsifier-producing microbial consortium

Impact of Irradiation and Polycyclic Aromatic Hydrocarbon Spiking on Microbial Populations in Marine Sediment for Future Aging and Biodegradability Studies

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