Enumeration of phenanthrene-degrading bacteria by an overlayer technique and its use in evaluation of petroleum-contaminated sites

Bogardt, Anne H.; Hemmingsen, Barbara B.

doi:10.1128/aem.58.8.2579-2582.1992

Cited by 110 publications

(26 citation statements)

References 13 publications

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“…Those that were turbid were scored as positive. Numbers of phenanthrene degraders were determined on agar overlay plates [13]. These were incubated at 16°C for 6 weeks, and colonies surrounded by a zone of clearing (indicative of phenanthrene degradation) were counted.…”

Section: Soil Analysesmentioning

confidence: 99%

Hydrocarbon contamination changes the bacterial diversity of soil from around Scott Base, Antarctica

Saul

Aislabie

Brown

et al. 2005

FEMS Microbiology Ecology

240

137

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A combination of culture-independent and culturing methods was used to determine the impacts of hydrocarbon contamination on the diversity of bacterial communities in coastal soil from Ross Island, Antarctica. While numbers of culturable aerobic heterotrophic microbes were 1-2 orders of magnitude higher in the hydrocarbon-contaminated soil than control soil, the populations were less diverse. Members of the divisions Fibrobacter/Acidobacterium, Cytophaga/Flavobacterium/Bacteroides, Deinococcus/Thermus, and Low G+C gram positive occurred almost exclusively in control soils whereas the contaminated soils were dominated by Proteobacteria; specifically, members of the genera Pseudomonas, Sphingomonas and Variovorax, some of which degrade hydrocarbons. Members of the Actinobacteria were found in both soils.

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Section: Soil Analysesmentioning

confidence: 99%

Hydrocarbon contamination changes the bacterial diversity of soil from around Scott Base, Antarctica

Saul

Aislabie

Brown

et al. 2005

FEMS Microbiology Ecology

240

137

View full text Add to dashboard Cite

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“…Stock solutions of each test compound were prepared in dimethylformamide at a concentration of either 5 or 10 mg ml −1 . The basal salts medium (BSM) contained (l −1 ): 0·4 g K 2 HPO 4 , 0·4 g KH 2 PO 4 , 0·4 g (NH 4 ) 2 SO 4 , 0·3 g NaCl, 5 ml trace elements solution, 5 ml vitamin solution ( Bogardt and Hemmingsen 1992) and 5 ml magnesium/calcium solution (0·4 g MgSO 4 .7H 2 O and 0·4 g CaCl 2 .2H 2 O). Vitamin, trace elements and magnesium/calcium solutions were sterilized by filtration (0·22 µm, Millipore) and added to BSM after autoclaving.…”

Section: Methodsmentioning

confidence: 99%

Enrichment and isolation of non-specific aromatic degraders from unique uncontaminated (plant and faecal material) sources and contaminated soils

Juhasz

Naidu

2000

J Appl Microbiol

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Microbial analysis of contaminated soil and uncontaminated plant and faecal material resulted in the enrichment of a number of microbial communities capable of utilizing a range of environmental pollutants. Growth was observed on polycyclic aromatic hydrocarbons, polychlorinated biphenyls, heterocyclic aromatic compounds and organochlorine pesticides. However, none of the communities could grow on pentachlorophenol. Pure cultures were isolated from microbial communities using phenanthrene and pyrene as the sole carbon and energy source. Isolates were also obtained using DDT, DOH, DBH and PCPA when peptone was supplemented to the medium. Strain AJR39,504, isolated using DDT and peptone, could not be positively identified on the basis of substrate utilization tests. However, it most closely resembled Stenotrophomonas maltophilia (0·424 similarity) using the Microlog 3 database software. Isolate AJR39,504 could also grow on polycyclic aromatic hydrocarbons, chlorinated‐ and nitro‐aromatic compounds. In addition, the degradation of DDT (100 mg l−1) by isolate AJR39,504 resulted in a 35% decrease in DDT concentration after 28 days with a concomitant increase in DDD concentration.

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“…17 rpm) 30 min; serial dilutions (sterile water) Grossbard and Hall (1964) 50 g soil + 450 ml 0.001 M Tris buffer; waring blender (16-18,000 rpm) for 1-5 min. Kanazawa, Takeshima, and Ohta (1986) 1 g soil + 24 ml filtered, reverse osmosis water; shake by hand for 1 min, stand 2 min; upper 10 ml removed and serial dilutions were prepared Bogardt and Hemmingsen (1992) 10 g soil + 100 ml sterile 0.2% tetrasodium pyrophosphate; shake 150 rpm for 30 min, stand 2-5 min. Klein and Dott (1992) 1 g soil + 9 ml phosphate buffer; shake 350 rpm for 15 min, stand 1 min.…”

Section: Soil Samplingmentioning

confidence: 99%

A research note on the assessment of optimum conditions for preparing soils for bioremediation feasibility testing

Guerin¹

2019

Remediation Journal

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In this applied study, the effects of short‐term storage at 22°C, 6°C, and −25°C on the numbers of microorganisms enumerated were examined with soils collected from a petrochemical contaminated soil containing multiple contaminants including phenol, polycyclic aromatic hydrocarbons, and petroleum hydrocarbons. Short term storage of soils at refrigerator temperature did not significantly change the number of microorganisms compared to those in the fresh soil (0 days of storage); however, at −25°C there was a slight decrease in the phenol utilizers and total viable count (TVC). Long‐term storage caused a significant decrease in the number of phenol utilizers in the petrochemical‐contaminated soil samples. Chemical dispersing agents were used in an attempt to increase the extraction of microorganisms from naphthalene contaminated soil which were predominantly clay soils. These did not significantly change the enumeration of naphthalene utilizers or TVC. While these results are not unexpected from current research and knowledge of microbial community succession in laboratory environments, the results from the applied nature of this study confirm that it is best practice to keep soil samples designated for bacterial enumeration for the shortest possible time, and not longer than 1–2 weeks, and at refrigerated temperature (6°C) in preference to room (22°C) or deep freezer (−18°C) temperatures.

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Enumeration of phenanthrene-degrading bacteria by an overlayer technique and its use in evaluation of petroleum-contaminated sites

Cited by 110 publications

References 13 publications

Hydrocarbon contamination changes the bacterial diversity of soil from around Scott Base, Antarctica

Hydrocarbon contamination changes the bacterial diversity of soil from around Scott Base, Antarctica

Enrichment and isolation of non-specific aromatic degraders from unique uncontaminated (plant and faecal material) sources and contaminated soils

A research note on the assessment of optimum conditions for preparing soils for bioremediation feasibility testing

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