Enumeration of Vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization

Griffitt, Kimberly J.; Noriea, Nicholas F.; Johnson, Crystal N.; Grimes, D. Jay

doi:10.1016/j.mimet.2011.02.006

Cited by 33 publications

(23 citation statements)

References 24 publications

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“…Since the VBNC state was proposed, more than 60 species have been demonstrated to enter the VBNC state [25, 38]. The environmental conditions required to enter the VBNC state differ between bacterial species.…”

Section: Discussionmentioning

confidence: 99%

Formation and Resuscitation of Viable but NonculturableSalmonella typhi

Zeng

Zhao

Cao

et al. 2013

BioMed Research International

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Salmonella typhi is a pathogen that causes the human disease of typhoid fever. The aim of this study was to investigate the viable but nonculturable (VBNC) state of S. typhi. Some samples were stimulated at 4°C or −20°C, while others were induced by different concentrations of CuSO4. Total cell counts remained constant throughout several days by acridine orange direct counting; however, plate counts declined to undetectable levels within 48 hours by plate counting at −20°C. The direct viable counts remained fairly constant at this level by direct viable counting. Carbon and nitrogen materials slowly decreased which indicated that a large population of cells existed in the VBNC state and entered the VBNC state in response to exposure to 0.01 or 0.015 mmol/L CuSO4 for more than 14 or 12 days, respectively. Adding 3% Tween 20 or 1% catalase enabled cells to become culturable again, with resuscitation times of 48 h and 24 h, respectively. The atomic force microscope results showed that cells gradually changed in shape from short rods to coccoids, and decreased in size when they entered the VBNC state. Further animal experiments suggested that resuscitated cells might regain pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Formation and Resuscitation of Viable but NonculturableSalmonella typhi

Zeng

Zhao

Cao

et al. 2013

BioMed Research International

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“…When environmental conditions no longer favour the growth of V . parahaemolyticus , the bacterium enters a dormant state known as viable but non‐culturable (VBNC) (Coutard et al ., ; Griffit et al ., ). In the VBNC state cells continue to be metabolically active but at greatly reduced rates, pathogenic strains remain virulent and the organism will not grow on artificial laboratory media (Oliver, ).…”

Section: Introductionmentioning

confidence: 97%

Vibrio parahaemolyticus type IV pili mediate interactions with diatom‐derived chitin and point to an unexplored mechanism of environmental persistence

Frischkorn

Stojanovski

Paranjpye

2013

Environmental Microbiology

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Vibrio parahaemolyticus is a naturally occurring bacterium common in coastal waters where it concentrates in shellfish through filter feeding. The bacterium is a human pathogen and the leading cause of seafood-borne gastroenteritis. Presently there is little information regarding mechanisms of environmental persistence of V.parahaemolyticus or an accurate early warning system for outbreak prediction. Vibrios have been shown to adhere to several substrates in the environment, including chitin, one of the most abundant polymers in the ocean. Diatoms are abundant in estuarine waters and some species produce chitin as a component of the silica cell wall or as extracellular fibrils. We examined the role of specific surface structures on the bacterium, the type IV pilins PilA and MshA, in adherence to diatom-derived chitin. Biofilm formation and adherence of V.parahaemolyticus to chitin is mediated by the ability of the bacterium to express functional type IV pili. The amount of adherence to diatom-derived chitin is controlled by increased chitin production that occurs in later stages of diatom growth. The data presented here suggest late-stage diatom blooms may harbour high concentrations of V.parahaemolyticus and could serve as the foundation for a more accurate early warning system for outbreaks of this human pathogen.

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“…Among them, Vibrio parahaemolyticus has become a primary causative factor of food-borne disease outbreaks in coastal countries worldwide (Zarei et al 2012), including China, Japan, India, Thailand, Australia, and the United States (Wu et al 2014;Raghunath et al 2008;Iwamoto et al 2010;WHO/FAO 2011;Yu et al 2013): this affects the safety of seafood and the health of consumers. Vibrio parahaemolyticus is a natural bacterium in marine and estuarine environments, the animals carrying V. parahaemolyticus could be the principal vehicle transmitting the pathogenic bacterium to humans, including aquatic farmed animals (Griffitt et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Liu

Wang

Lǐ

et al. 2015

World J Microbiol Biotechnol

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Acute gastroenteritis caused by pathogenic Vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. Using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. In this study, a total of 249 isolates were isolated from four types of seafood (Litopenaeus vannamei, Oratosquilla oratoria, Mactra veneriformis and Portunus trituberculatus) and coastal sediment from Liaodong Bay in the Bohai Sea, China with five different separation agars. The most isolates came from the sample of coastal sediment and on agar of 2216E, which accounted for 36.14 and 54.62 % respectively. Twenty-four among 249 isolates displayed direct antimicrobial activity to V. parahaemolyticus with spot inoculation. Sixteen active isolates were selected for extracellular antimicrobial activity using the Oxford cup method. Only strains of B16 and J7 showed extracellular antimicrobial activity and were identified as Bacillus pumilus and Bacillus mojavensis respectively based on the physiological identification and 16S rRNA sequence analysis. Both of the strains B16 and J7 exhibited extracellular hydrolytic enzyme activity and antagonism against more than one indicator bacteria in vitro, which indicates that the two strains have broad potential application as suitable probiotic candidates in aquaculture while B. mojavensis was first reported to inhibit pathogenic Vibrio spp. in vitro. There is no particular trait as to antagonism of B. pumilus B16 or B. mojavensis J7 to Gram-positive or Gram-negative indicator bacteria.

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Enumeration of Vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization

Cited by 33 publications

References 24 publications

Formation and Resuscitation of Viable but NonculturableSalmonella typhi

Formation and Resuscitation of Viable but NonculturableSalmonella typhi

Vibrio parahaemolyticus type IV pili mediate interactions with diatom‐derived chitin and point to an unexplored mechanism of environmental persistence

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

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