Environmental applications of microbial extremophiles in the degradation of petroleum hydrocarbons in extreme environments

Rajkumari, Jina; Bhuyan, Bhrigu; Das, Nandita; Pandey, Piyush

doi:10.1007/s42398-019-00065-1

Cited by 15 publications

(6 citation statements)

References 139 publications

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“…Thermophiles have attracted considerable attention because they present specific features with biotechnological and industrial interest, such as the production of different biomolecules (exopolysaccharide, antimicrobial, biosurfactant) [3][4][5] and thermostable enzymes (amylases, cellulases, chitinases, pectinases, xylanases, proteases, lipase, and DNA polymerases), for biotechnological applications in medical, industrial, and agriculture processes [1]. These organisms may also serve in an increasing number of potential bioremediation applications, mainly removing of heavy metals from waste [6], biodegradation of petroleum hydrocarbons, [7], and in renewable energy [8].…”

Section: Introductionmentioning

confidence: 99%

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme activities were also investigated. A total of 293 strains were isolated from the hot springs' water and sediment using different culture media. Overall, five distinct bacterial groups were characterized by whole-cell protein pattern analysis. Based on the 16S rRNA gene sequencing of 100 selected strains, the isolates were assigned to the following three major phyla: Firmicutes (93%), Deinococcus-Thermus (5%), and Actinobacteria (2%), which included 27 distinct species belonging to 12 different phylotypes, Aeribacillus, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, Laceyella, Meiothermus, Saccharomonospora, Thermoactinomyces, Thermobifida, and Thermus. The screening for nine extracellular enzymes showed that 65.87% of the isolates presented at least five types of enzyme activities, and 6.48% of strains combined all tested enzymes (amylase, cellulase, pectinase, esculinase, protease, gelatinase, lipase, lecithinase, and nuclease). It was found that Bacillus, Anoxybacillus, Aeribacillus, and Aneurinibacillus were the genera showing the highest activities. Likewise, the study showed an abundant and diverse thermophilic community with novel taxa presenting a promising source of thermozymes with important biotechnological applications. This study showed that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully differentiate thermophilic bacteria from Algerian hot springs.

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Section: Introductionmentioning

confidence: 99%

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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“…The fungi belonging to species like Aspergillus and Candida excrete enzymes called laccases and peroxidases, which are used to degrade hydrocarbons in the process by producing reactive oxygen species to form oxidative cleavages of carbon-carbon bonds (Daccò, C. 2021). The microbial strains of metabolically flexible techniques can adapt to various environmental conditions, including temperature, pH, and nutrient levels, which makes these strains efficient hydrocarbon degraders in different habitats like the ocean, soil, and contaminated sites (Rajkumari, J., et al, 2019).…”

Section: Metabolic Pathways Of Key Microbial Strainsmentioning

confidence: 99%

Petroleum Hydrocarbons Biodegradation Uncovering the Variety and Capabilities of Oil-Oxidizing Microbes

Hassand,

Omirbekova,

Baseer

et al. 2024

ejtas

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The biodegradation of petroleum hydrocarbons is a valuable process used to reduce the ecological influences of oil spills and pollution. This comprehensive review immerses readers in the sophisticated universe of oil-oxidizing organisms, the diversity and functionality of which are unveiled. By examining different bacterial groups, such as aerobic and anaerobic bacteria, fungi, archaea, and algae, the study shows enzymatic and metabolic processes exploited during biodegradation. Environmental factors, substrate characteristics, and microbial interactions are the main determinants that contribute to the good performance of the biodegradation of petroleum hydrocarbons. The effectiveness of various biotechnological strategies like in-situ and ex-situ bioremediation, bioaugmentation, and bio stimulation is being tested to determine their ability to embrace the microbial capabilities for environmental restoration.

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“…In addition, thermophilic bacteria have several other biotechnological applications, including the production of bioactive compounds (e.g., exopolysaccharides, antibiotics, and biosurfactants) ( Nicolaus et al 2010 ; Mahajan and Balachandran 2017 ; Schultz et al 2022 ). Moreover, they can be used for the removal of heavy metals by bioremediation as well as for the biodegradation of plastic polymers and hydrocarbons ( Ilyas et al 2014 ; Rajkumari et al 2019 ; Ru et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Insights into Genomic Features and Potential Biotechnological Applications of Bacillus halotolerans Strain HGR5

Yahiaoui,

Bounabi,

Boukerb

et al. 2023

Polish Journal of Microbiology

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Algeria is one of the wealthiest countries in terms of hydrothermal sources, with more than two hundred hot springs. However, diverse and little-described microbial communities colonize these habitats, making them an intriguing research subject. This work reports the isolation of bacteria from two hot springs water samples in northeastern Algeria, evaluating their enzymatic activities and effect on plant pathogens. Out of the obtained 72 bacterial isolates and based on the 16S rRNA gene sequence analysis, the strain HGR5 belonging to Bacillus halotolerans had the most interesting activity profile. Interestingly, HGR5 was substantially active against Fusarium graminearum, Phytophthora infestans, and Alternaria alternata. Furthermore, this strain presented a high ability to degrade casein, Tween 80, starch, chitin, cellulose, and xylan. The genome sequence of HGR5 allowed taxonomic validation and screening of specific genetic traits, determining its antagonistic and enzymatic activities. Genome mining revealed that strain HGR5 encloses several secondary metabolite biosynthetic gene clusters (SM-BGCs) involved in metabolite production with antimicrobial properties. Thus, antimicrobial metabolites included bacillaene, fengycin, laterocidine, bacilysin, subtilosin, bacillibactin, surfactin, myxovirescin, dumulmycin, and elansolid A1. HGR5 strain genome was also mined for CAZymes associated with antifungal activity. Finally, the HGR5 strain exhibited the capacity to degrade polycaprolactone (PCL), a model substrate for polyester biodegradation. Overall, these results suggest that this strain may be a promising novel biocontrol agent with interesting plastic-degradation capability, opening the possibilities of its use in various biotechnological applications.

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Environmental applications of microbial extremophiles in the degradation of petroleum hydrocarbons in extreme environments

Cited by 15 publications

References 139 publications

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

Petroleum Hydrocarbons Biodegradation Uncovering the Variety and Capabilities of Oil-Oxidizing Microbes

Insights into Genomic Features and Potential Biotechnological Applications of Bacillus halotolerans Strain HGR5

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