Environmental biodegradability of recombinant structural protein

Tachibana, Yuya; Darbe, Sunita; Hayashi, Senri; Kudasheva, Alina; Misawa, Haruna; Shibata, Yuka; Kasuya, Kazuhiko

doi:10.1038/s41598-020-80114-6

Cited by 13 publications

(5 citation statements)

References 39 publications

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“…Mineralization was evaluated through biochemical oxygen demand (BOD)-biodegradation tests with soil inocula. The BOD biodegradability of BP1 reached 70.2 ± 6.0 after 33 days, as reported by Tachibana et al [122].…”

Section: Biodegradabilitysupporting

confidence: 83%

Protein-Based Films and Coatings: An Innovative Approach

Purewal,

Kaur,

Bangar

et al. 2023

Coatings

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Protein-based films and coatings are highly biodegradable and represent sustainable alternatives to petroleum-based materials. These materials possess commendable barrier properties, effectively safeguarding against oxygen, moisture, and aroma compounds, rendering them well-suited for various food packaging applications. Beyond their role in food packaging, coatings and films have significant applications in the biomedical and pharmaceutical domains. Their inherent biocompatibility and controlled release properties make them valuable for applications such as drug-delivery systems, wound dressings, and tissue-engineering scaffolds. Moreover, the adaptability of these films to exhibit stimuli-responsive behavior opens avenues for on-demand drug release and sensing capabilities. Despite these promising attributes, challenges persist in terms of the mechanical strength, water resistance, and scalability of the processing of protein-based films and coatings. Ongoing research endeavors are dedicated to refining protein extraction methods, incorporating reinforcing agents, and implementing strategies to optimize the overall performance of these materials. Such efforts aim to overcome existing limitations and unlock the full potential of protein-based films and coatings in diverse applications, contributing to the advancement of sustainable and versatile biomaterials.

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Section: Biodegradabilitysupporting

confidence: 83%

Protein-Based Films and Coatings: An Innovative Approach

Purewal,

Kaur,

Bangar

et al. 2023

Coatings

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“…Biodegradability is one of the key desirable aspects of conventional plastic alternatives today. Conventional protein-based bioplastics have been shown to have high degradability under a short period, 162 compared to synthetic plastics. 163 However, little is known about the biodegradability of amyloid-based protein bioplastics.…”

Section: Properties and Applicationsmentioning

confidence: 99%

From Fundamental Amyloid Protein Self-Assembly to Development of Bioplastics

Li,

Kambanis,

Sorenson

et al. 2023

Biomacromolecules

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Proteins can self-assemble into a range of nanostructures as a result of molecular interactions. Amyloid nanofibrils, as one of them, were first discovered with regard to the relevance of neurodegenerative diseases but now have been exploited as building blocks to generate multiscale materials with designed functions for versatile applications. This review interconnects the mechanism of amyloid fibrillation, the current approaches to synthesizing amyloid protein-based materials, and the application in bioplastic development. We focus on the fundamental structures of self-assembled amyloid fibrils and how external factors can affect protein aggregation to optimize the process. Protein self-assembly is essentially the autonomous congregation of smaller protein units into larger, organized structures. Since the properties of the self-assembly can be manipulated by changing intrinsic factors and external conditions, protein self-assembly serves as an excellent building block for bioplastic development. Building on these principles, general processing methods and pathways from raw protein sources to mature state materials are proposed, providing a guide for the development of large-scale production. Additionally, this review discusses the diverse properties of protein-based amyloid nanofibrils and how they can be utilized as bioplastics. The economic feasibility of the protein bioplastics is also compared to conventional plastics in large-scale production scenarios, supporting their potential as sustainable bioplastics for future applications. P lastics are now found in every sector of our life, such as packaging, personal care products, electronics, and clothing, while the contamination caused by the accumulation of single-use plastic 1−5 and microplastics 6,7 has become a serious threat to the natural environment, wildlife, and humans. Current plastics can be classified based on their source and their biodegradability, as shown in Figure 1. 8 Some plastics are

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“…Biodegradation studies therefore have to be conducted in order to verify the degradation potential and speed in the natural environment. A study on compression-molded sheets [ 27 ] made from Spiber's Brewed Protein™ using soil-based inoculum shows quick degradation to oligopeptides and amino acids. Furthermore, biodegradation of Spiber's Brewed Protein™ fibers in seawater shows a fast and high level of mineralization, comparable to the known biodegradable compound succinic acid over 40 days ( Figure 2 ), suggesting high ultimate biodegradability in marine conditions.…”

Section: Responsible End-of-lifementioning

confidence: 99%

Practical considerations for delivering on the sustainability promise of fermentation-based biomanufacturing

Lips

2021

Emerging Topics in Life Sciences

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Increasingly, bio-based products made via sugar-powered microbial cell factories and industrial fermentation are reaching the market and presenting themselves as sustainable alternatives to fossil and animal-based products. The sustainability potential of biotechnology, however, has been shown to come with trade-offs and cannot be taken for granted. Shared environmental impact hotspots have been identified across industrial fermentation-based products, including biomass production, energy consumption, and end-of-life fate. Based on both these patterns and our direct experience in preparing for the commercial-scale production of Brewed Protein™, we outline practical considerations for improving the sustainability performance of bio-based products made via industrial fermentation.

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Environmental biodegradability of recombinant structural protein

Cited by 13 publications

References 39 publications

Protein-Based Films and Coatings: An Innovative Approach

Protein-Based Films and Coatings: An Innovative Approach

From Fundamental Amyloid Protein Self-Assembly to Development of Bioplastics

Practical considerations for delivering on the sustainability promise of fermentation-based biomanufacturing

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