Environmental control of adventitious rooting in Eucalyptus and Populus cuttings

Almeida, Márcia Rodrigues de; Aumond, Márcio L.; Costa, Cibele Tesser da; Schwambach, Joséli; Ruedell, Carolina Michels; Correa, L. R.; Fett-Neto, Arthur Germano

doi:10.1007/s00468-017-1550-6

Cited by 59 publications

(30 citation statements)

References 95 publications

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“…Previous experiments showed that leaf cuttings with axillary buds were able to form new plants, like citrus, Eucalyptus, Populus plants, etc. [29,30]. Our findings concur with the existing understanding of a low rooting rate being the primary challenge facing Chinese-fir propagation through leaf cuttings.…”

Section: Discussionsupporting

confidence: 88%

Effects of Leaf Age and Exogenous Hormones on Callus Initiation, Rooting Formation, Bud Germination, and Plantlet Formation in Chinese Fir Leaf Cuttings

Zhou

Huang

et al. 2018

Forests

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To guide the cultivation of superior Chinese fir plantlets, we designed an L16(4)4 orthogonal experiment to determine how leaf age and exogenous hormones influence key growth processes in leaf cuttings. Hormone concentration and treatment duration significantly affected leaf cuttings in all three age categories; 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA), and treatment time exerted the strongest effects on callus initiation rates. Additionally, NAA had the largest effect on the rooting rate across all cuttings, and all three hormones significantly influenced the bud germination rate. Based on our experimental results, expected optimal treatments for callus initiation were 10 mg∙L−1 indole-3-butyric acid (IBA) for 10 min, 30 mg∙L−1 NAA for 15 min, and 10 mg∙L−1 NAA plus 30 mg∙L−1 IBA for 10 min. For the rooting rate, the expected optimal treatment was 50 mg∙L−1 NAA and 40 mg∙L−1 IBA for 5–20 min. Finally, for bud germination, optimal treatments were 20 min of immersion in water, 30 mg∙L−1 6-BA plus 50 mg∙L−1 NAA for 15 min, and 30 mg∙L−1 6-BA for 5 min. Plantlet formation only occurred in the <one-year-old leaves, and at very low rates (maximum 5.8%); this outcome is likely attributable to the mother plant’s relatively old age (five years). Plantlet formation from cuttings is dependent on ensuring the rooting rate after callus initiation. Therefore, to promote rooting rates and bud germination, we recommend leaving more xylem at the base of leaf cuttings.

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Section: Discussionsupporting

confidence: 88%

Effects of Leaf Age and Exogenous Hormones on Callus Initiation, Rooting Formation, Bud Germination, and Plantlet Formation in Chinese Fir Leaf Cuttings

Zhou

Huang

et al. 2018

Forests

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“…Detailed knowledge about the growth behaviour, ecophysiological and biochemical responses and the subspecifi c variation of eucalypt in water stress conditions could contribute to the selection of promising species and the success of reforestation programs. However, after the selection of resistant species, we can plant them in arid regions on marginal soils using treated wastewater for irrigation (De Almeida et al, 2017) because wastewater irrigation can improve the growth and productivity of Eucalyptus plantation wood (Minhas et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

An overview of adaptative responses to drought stress in Eucalyptus spp.

et al. 2017

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Eucalyptus is a plant frequently present and cultivated in arid regions because of its high adaptation to drought. Furthermore, it is known by its numerous species which represents a great opportunity to choose several tolerant species with variable uses. Therefore, the choice of species, hybrids or genotypes resistant to aridity becomes a necessity before any plantation in regions and countries characterized by semi-arid, arid and Saharan climates. Our review shows an important effect of drought on anatomical, physiological and biochemical parameters of Eucalyptus spp. Indeed, water stress acts directly on growth, yield and response to pests. However, the response to drought varies significantly among species, genotypes, hybrids and clones. This result represents a field for the selection of eucalypts tolerant and adapted to water deficit or climate change.

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“…Auxin is also known for regulating different downstream factors that can affect AR development, such as nitric oxide and microtubule-related transcripts ( Steffens and Rasmussen, 2016 ; De Almeida et al, 2017 ). Juvenile cuttings derived from 6-month old plants of E. grandis showed higher nitric oxide production ( Abu-Abied et al, 2012 ) and increased responsiveness of microtubule-related transcripts upon auxin treatment ( Abu-Abied et al, 2014 ) compared to their mature counterparts.…”

Section: Discussionmentioning

confidence: 99%

Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

et al. 2017

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The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in E. globulus, the time required for a significant decline in rhizogenic ability without exogenous auxin was determined in microcuttings derived from donor plants of different ages after sowing. Tip cuttings of donor plants were severed before and after loss of rooting competence of microcuttings to test the hypothesis that auxin and carbohydrate homeostasis regulate rooting competence decline. There were no significant changes in concentration of carbohydrates, flavonoids, or proteins before and after the loss of rooting capacity. Peroxidase (EC 1.11.1.7) total activity increased with loss of rooting competence. Auxin concentration showed the opposite pattern. In good agreement, TAA1, a key gene in auxin biosynthesis, had lower expression after loss of rooting capacity. The same applied to the auxin receptor gene TIR1, suggesting reduced auxin sensitivity. On the other hand, genes associated with auxin response repression (TPL, IAA12) or with the action of cytokinins, the rhizogenesis inhibitor-related ARR1, showed higher expression in plants with lower rooting competence. Taken together, data suggest that age negatively affects E. globulus rooting by a combination of factors. Decreased endogenous auxin concentration, possibly caused by less biosynthesis, lower auxin sensitivity, higher expression of genes inhibiting auxin action, as well as of genes related to the action of cytokinins, appear to play roles in this process.

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Environmental control of adventitious rooting in Eucalyptus and Populus cuttings

Cited by 59 publications

References 95 publications

Effects of Leaf Age and Exogenous Hormones on Callus Initiation, Rooting Formation, Bud Germination, and Plantlet Formation in Chinese Fir Leaf Cuttings

Effects of Leaf Age and Exogenous Hormones on Callus Initiation, Rooting Formation, Bud Germination, and Plantlet Formation in Chinese Fir Leaf Cuttings

An overview of adaptative responses to drought stress in Eucalyptus spp.

Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

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