Environmental DNA as an efficient tool for detecting invasive crayfishes in freshwater ponds

Mauvisseau, Quentin; Coignet, Aurore; Delaunay, Carine; Pinet, François; Bouchon, Didier; Souty‐Grosset, Catherine

doi:10.1007/s10750-017-3288-y

Cited by 52 publications

(54 citation statements)

References 66 publications

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“…To date, a handful of crayfish eDNA studies have shown promising results but have largely focused on lentic environments (e.g. Agersnap et al., ; Cai et al., ; Dougherty et al., ; Mauvisseau et al., ; Tréguier et al., ), although one study (Ikeda, Doi, Tanaka, Kawai, & Negishi, ) used eDNA to successfully detect the endemic Japanese crayfish ( Cambaroides japonicus ) in small headwater streams. Despite these encouraging results, it remains to be seen whether eDNA can reflect presence and abundance of narrowly endemic crayfishes in larger, perennial streams where eDNA transport is more likely to be a problem (Deiner & Altermatt, ).…”

Section: Introductionmentioning

confidence: 99%

“…To date, a handful of crayfish eDNA studies have shown promising results but have largely focused on lentic environments (e.g. Agersnap et al, 2017;Cai et al, 2017;Dougherty et al, 2016;Mauvisseau et al, 2017;Tr eguier et al, 2014), although one study (Ikeda, Doi, Tanaka, Kawai, & Negishi, 2016) used eDNA to successfully detect the endemic Japanese crayfish (Cambaroides japonicus) in small headwater streams.…”

Section: Introductionmentioning

confidence: 99%

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Environmental DNA detects a rare large river crayfish but with little relation to local abundance

2018

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Environmental DNA (eDNA) is DNA extracted from environmental samples (e.g. water) that can be used to infer presence or abundance of species, sometimes with greater sensitivity than conventional sampling methods. Previous eDNA applications to lotic ecosystems have shown promise in accurately inferring species presence, although studies attempting to estimate species abundance have had mixed results. This may be because eDNA applications in lotic environments are challenged by directional streamflow, which has the potential to transport detectable eDNA downstream from its source. Our study sought to evaluate whether results from eDNA corresponded well with the presence and abundance of the narrowly endemic, large river specialist crayfish Faxonius eupunctus obtained through a rigorous, well‐tested conventional sampling method, or instead, if downstream eDNA transport in this large river system might overwhelm the effect of local species abundance. We used a species‐specific quantitative PCR (qPCR) assay to amplify F. eupunctus eDNA collected in surface water samples from streams within the Eleven Point River drainage, Arkansas and Missouri, U.S.A. We estimated F. eupunctus eDNA detection probabilities and examined relationships between eDNA detection probability and site‐scale variables using a hierarchical occupancy and detection probability modelling framework. Results from eDNA sampling showed ˜90% agreement relative to our conventional sampling method in estimating F. eupunctus presence, although eDNA failed to detect F. eupunctus eDNA at two upstream sites where conventional sampling detected F. eupunctus individuals. We found a poor relationship between F. eupunctus eDNA detection probability and local F. eupunctus abundance, and a strong relationship between eDNA detection probability and upstream river distance, which we used as a proxy for the risk of downstream transport of eDNA from upstream F. eupunctus populations. Our results demonstrate eDNA is a largely reliable tool for estimating the presence of benthic organisms in large, freshwater rivers. However, the likelihood of detecting F. eupunctus eDNA presence in our study increased as we moved down the stream network, even though local species abundances were greatest at more upstream locations. Therefore, the ability of eDNA to accurately reflect species presence or abundance in some lotic environments may be hindered by the downstream transport of detectable eDNA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Environmental DNA detects a rare large river crayfish but with little relation to local abundance

2018

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“…In this context, further monitoring could be assisted with the development of eDNA techniques targeted for I. nubecula. eDNA is a relatively new tool available to conservationists, ecologists and environmental managers alike (Thomsen & Willerslev, 2014) and can significantly aid in understanding a species home range with reduced physical surveying needs and reduced costs (Mauvisseau et al, 2017). Access to this DNA sequence and a greater comprehension of the phylogenetic relationships (Fig.…”

Section: Resultsmentioning

confidence: 99%

Rediscovery of the critically endangered ‘scarce yellow sally stonefly’ Isogenus nubecula in United Kingdom after a 22 year period of absence

Davy‐Bowker

Hammett²,

Mauvisseau

et al. 2018

Zootaxa

Self Cite

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The critically endangered 'scarce yellow sally stonefly' Isogenus nubecula (Newman, 1833) (Plecoptera: Perlodidae) was rediscovered in the United Kingdom (UK) in 2017. This rediscovery comes after a 22-year period of absence despite numerous surveys since its last record in 1995. This species is one of the rarest stoneflies in the UK and Europe and its rediscovery is of international significance, being the westernmost point in Europe where the species is found, with the next nearest populations occurring in Austria and western Hungary, Slovakia, and central Sweden. The species is classed as pRDB2 (vulnerable), however is not listed in the British Red Data Book despite only being present (as far as records detail) in one river, the River Dee in North Wales, UK. Only fourteen individuals were caught and the need for conservation of this rare stonefly is therefore of paramount importance. We have made recommendations for the need to increase survey effort using environmental DNA (eDNA) techniques in order to fully understand the species range in this river and those in the surrounding area. The DNA sequence of I. nubecula has been uploaded on GenBank for further genetic studies. Captive rearing could also be explored with possible reintroductions to sites within its former UK range.

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“…In contrast to other essays developed for crayfish detection (Agersnap et al 2017; Cai et al 2017; Dougherty et al 2016; Mauvisseau et al 2018), our single, closed tube reaction, reduces not only the processing time and number of reactions but also the risk of contamination inherent to carry out a larger number of amplifications. HRM has already proved highly specific and useful for multiple species identification (Naue et al 2014) and for the management of terrestrial invasive species (Ramón-Laca et al 2014) but had never been applied to the detection of aquatic invasive species and their impacts using eDNA.…”

Section: Discussionmentioning

confidence: 97%

“…While most studies have mainly focussed on crayfish eDNA detection in closed systems (Agersnap et al 2017; Cai et al 2017; Dougherty et al 2016; Mauvisseau et al 2018), our method has also proved useful for monitoring in flowing water bodies. This is important for early detection of invasive crayfish which use rivers and streams as a means for dispersal (Bubb, Thom and Lucas 2004), and particularly for A. pallipes whose detection was marginally better using eDNA (7%) than trapping (0%).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous detection of invasive signal crayfish, endangered white-clawed crayfish and the crayfish plague pathogen using environmental DNA

Robinson

Uren-Webster

Cable

et al. 2018

Preprint

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Environmental DNA as an efficient tool for detecting invasive crayfishes in freshwater ponds

Cited by 52 publications

References 66 publications

Environmental DNA detects a rare large river crayfish but with little relation to local abundance

Environmental DNA detects a rare large river crayfish but with little relation to local abundance

Rediscovery of the critically endangered ‘scarce yellow sally stonefly’ Isogenus nubecula in United Kingdom after a 22 year period of absence

Simultaneous detection of invasive signal crayfish, endangered white-clawed crayfish and the crayfish plague pathogen using environmental DNA

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