Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America

Larson, Eric R.; Renshaw, Mark A.; Gantz, Crysta A.; Umek, John; Chandra, Sudeep; Lodge, David M.; Egan, Scott P.

doi:10.1007/s10750-017-3210-7

Cited by 80 publications

(70 citation statements)

References 53 publications

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“…Furthermore, eDNA can be stored in sediments for months and possibly years, which may then get flushed periodically during spring releases (e.g., Turner et al 2015). eDNA has been successfully used in very large freshwater habitats like the Great Lakes of North America, but evidence suggests that target species DNA was not well mixed across such large expanses (e.g., Jerde et al 2013, Larson et al 2017. Relative to comparable eDNA sampling within reservoirs, eDNA sampling at downstream streamgages may result in a high proportion of negatives, even when the target species is present in the reservoir.…”

Section: Discussionmentioning

confidence: 99%

“…If transport time is slow and water quality conditions are caustic to DNA (e.g., warm temperatures and high UV exposure; Pilliod et al 2014, Seymour et al 2018, then the target DNA may decay before it reaches a downstream streamgage. In these studies, positive eDNA detections were limited to <34 km and ≤4 km of previously observed localities for crayfish (Faxonius rusticus; Larson et al 2017) and Asian carp (Hypophthalmichthys spp. Reservoirs in the West, including those in this study, are also large.…”

Section: Discussionmentioning

confidence: 99%

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Adding invasive species biosurveillance to the U.S. Geological Survey streamgage network

et al. 2019

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The costs of invasive species in the United States alone are estimated to exceed US$100 billion per year, so a critical tactic in minimizing the costs of invasive species is the development of effective, early-detection systems. To this end, we evaluated the efficacy of adding environmental (e)DNA surveillance to the U.S. Geological Survey (USGS) streamgage network, which consists of >8200 streamgages nationwide systemically visited by USGS hydrologic technicians. Incorporating strategic eDNA sample collection during routine streamgage visits could provide early-detection surveillance of aquatic invasive species with minimal additional cost. For this evaluation, USGS hydrologic technicians collected monthly eDNA water samples, May-September 2018, from streamgages downstream of reservoirs in the Columbia River Basin thought to be vulnerable to invasive dreissenid mussel (Dreissenidae spp.) establishment. We tested water samples for dreissenid mussel DNA and also for kokanee (Oncorhynchus nerka) and yellow perch (Perca flavescens) DNA; the two fishes were used to assess if streamgages are adequately located to provide early-detection eDNA surveillance of taxa known to be present in upstream reservoirs. No Columbia River Basin streamgage samples met our criteria for being scored as positive for dreissenid DNA. We did detect kokanee and yellow perch DNA at all streamgages downstream of reservoirs where these species are known to occur. Field collection, laboratory analyses, and personnel time required for collection of four eDNA samples at a streamgage site cost US$500-US$600 (net). Given these results, incorporating eDNA biosurveillance into routine streamgage visits might decrease costs associated with an invasion since early detection maximizes the potential for eradication, containment, and mitigation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Adding invasive species biosurveillance to the U.S. Geological Survey streamgage network

et al. 2019

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“…In general, eDNA has performed well for representing occupancy of species (Dougherty et al., ; Pilliod et al., ; Smart et al., ), and some studies have found strong agreement between abundance or biomass of organisms and abundance of eDNA in the environment (Doi et al., ; Klobucar et al., ). Specific to crayfishes, studies have generally found eDNA to accurately represent occupancy of crayfishes, potentially with more sensitivity than some conventional methods, but eDNA has performed poorly in reflecting crayfish abundance (Agersnap et al., ; Cai et al., ; Dougherty et al., ; Larson et al., ). In some of these cases, the poor ability of eDNA to estimate abundance relative to conventional sampling methods could be related to weaknesses of the conventional methods themselves; for example, baited trapping of crayfishes has known biases and limitations that could fail to accurately reflect crayfish biomass (Larson & Olden, ; Stuecheli, ).…”

Section: Discussionmentioning

confidence: 99%

Environmental DNA detects a rare large river crayfish but with little relation to local abundance

2018

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Environmental DNA (eDNA) is DNA extracted from environmental samples (e.g. water) that can be used to infer presence or abundance of species, sometimes with greater sensitivity than conventional sampling methods. Previous eDNA applications to lotic ecosystems have shown promise in accurately inferring species presence, although studies attempting to estimate species abundance have had mixed results. This may be because eDNA applications in lotic environments are challenged by directional streamflow, which has the potential to transport detectable eDNA downstream from its source. Our study sought to evaluate whether results from eDNA corresponded well with the presence and abundance of the narrowly endemic, large river specialist crayfish Faxonius eupunctus obtained through a rigorous, well‐tested conventional sampling method, or instead, if downstream eDNA transport in this large river system might overwhelm the effect of local species abundance. We used a species‐specific quantitative PCR (qPCR) assay to amplify F. eupunctus eDNA collected in surface water samples from streams within the Eleven Point River drainage, Arkansas and Missouri, U.S.A. We estimated F. eupunctus eDNA detection probabilities and examined relationships between eDNA detection probability and site‐scale variables using a hierarchical occupancy and detection probability modelling framework. Results from eDNA sampling showed ˜90% agreement relative to our conventional sampling method in estimating F. eupunctus presence, although eDNA failed to detect F. eupunctus eDNA at two upstream sites where conventional sampling detected F. eupunctus individuals. We found a poor relationship between F. eupunctus eDNA detection probability and local F. eupunctus abundance, and a strong relationship between eDNA detection probability and upstream river distance, which we used as a proxy for the risk of downstream transport of eDNA from upstream F. eupunctus populations. Our results demonstrate eDNA is a largely reliable tool for estimating the presence of benthic organisms in large, freshwater rivers. However, the likelihood of detecting F. eupunctus eDNA presence in our study increased as we moved down the stream network, even though local species abundances were greatest at more upstream locations. Therefore, the ability of eDNA to accurately reflect species presence or abundance in some lotic environments may be hindered by the downstream transport of detectable eDNA.

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“…Thermal flexibility has also been reported in crayfishes (Haubrock et al, 2019;Veselý, Buřič, & Kouba, 2015); for instance, Veselý et al (2015) reported from laboratory experi- (Larson et al, 2017) and P. clarkii (Tréguier et al, 2014). In addition, niche modelling cannot consider thermal flexibility of some species.…”

Section: Potential Distributions Under Current and Future Climate Smentioning

confidence: 99%

Impacts of climate change on the global potential distribution of two notorious invasive crayfishes

et al. 2019

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1. Invasive alien species and climate change are two of the most serious global environmental threats. In particular, it is of great interest to understand how changing climates could impact the distribution of invaders that pose serious threats to ecosystems and human activities.2. In this study, we developed ensemble species distribution models for predicting the current and future global distribution of the signal crayfish Pacifastacus leniusculus and the red swamp crayfish Procambarus clarkii, two of the most highly problematic invaders of freshwater ecosystems worldwide. We collected occurrence records of the species, from native and alien established ranges worldwide.These records in combination with averaged observations of current climatic conditions were used to calibrate a set of 10 distinct correlative models for estimating the climatic niche of each species. We next projected the estimated niches into the geographical space for the current climate conditions and for the 2050s and 2070s under representative concentration pathway 2.6 and 8.5 scenarios.3. Our species distribution models had high predictive abilities and suggest that annual mean temperature is the main driver of the distribution of both species.Model predictions indicated that the two crayfish species have not fully occupied their suitable climates and will respond differently to future climate scenarios in different geographic regions. Suitable climate for P. leniusculus was predicted to shift poleward and to increase in extent in North America and Europe but decrease in Asia. Regions with suitable climate for P. clarkii are predicted to widen in Europe but contract in North America and Asia.4. This study highlights that invasive species with different thermal preference are likely to respond differently to future climate changes. Our results provide important information for policy makers to design and implement anticipated measures for the prevention and control of these two problematic species. K E Y W O R D Sclimate change, habitat suitability, Pacifastacus leniusculus, Procambarus clarkii, species distribution modelling

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Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America

Cited by 80 publications

References 53 publications

Adding invasive species biosurveillance to the U.S. Geological Survey streamgage network

Adding invasive species biosurveillance to the U.S. Geological Survey streamgage network

Environmental DNA detects a rare large river crayfish but with little relation to local abundance

Impacts of climate change on the global potential distribution of two notorious invasive crayfishes

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