A b s t r a c t Biomass reduction and amino acid content in plants of). The fresh weight of weeds and the content of free branched-chain amino acids (valine, leucine and isoleucine) in plant tissue were recorded. Tribenuron methyl was the herbicide that most limited biomass productivity, regardless of weed species and climate conditions. S. media was more sensitive to herbicides than P. rhoeas. Weed response to herbicides was dependent on temperature, but not on relative humidity. Tribenuron methyl applied to both weed species under various temperature regimes caused significant amino acid deficiency. The reduction in amino acid content in plants of P. rhoeas was greater at warm temperature compared to the cold regime due to stronger reaction to tribenuron methyl applied under these conditions. In most of cases, the mixture of 2.4-D + dicamba induced overproduction of amino acids.