Environmental enrichment alters LPS-induced changes in BDNF and PSD-95 expressions during puberty

Murack, Michael; Smith, Kevin B.; Traynor, Olivia H.; Pirwani, Atiqa F.; Gostlin, Sarah; Taha, Mohamed; Tata, Despina Α.; Messier, Claude; Ismail, Nafissa

doi:10.1016/j.brainres.2023.148283

Cited by 8 publications

(6 citation statements)

References 83 publications

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“…However, regarding CA3, EE increased SYN expression in both males and females. Although these findings are in agreement with previous studies supporting the beneficial role of EE on synaptic plasticity (Dandi et al., 2018; Murack et al., 2023), the sex‐dependent effects of EE housing during adolescence on specific markers of synaptic plasticity have been less investigated. Kokras et al.…”

Section: Discussionsupporting

confidence: 90%

Environmental enrichment initiated in adolescence restores the reduced expression of synaptophysin and GFAP in the hippocampus of chronically stressed rats in a sex‐specific manner

Dandi,

Theotokis,

Petri

et al. 2023

Developmental Psychobiology

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This study aims at investigating whether environmental enrichment (EE) initiated in adolescence can alter chronic unpredictable stress (CUS)‐associated changes in astroglial and synaptic plasticity markers in male and female rats. To this end, we studied possible alterations in hippocampal glial fibrillary acidic protein (GFAP) and synaptophysin (SYN) in CUS rats previously housed in EE. Wistar rats on postnatal day (PND) 23 were housed for 10 weeks in standard housing (SH) or enriched conditions. On PND 66, animals were exposed to CUS for 4 weeks. SYN and GFAP expressions were evaluated in CA1 and CA3 subfields and dentate gyrus (DG). CUS reduced the expression of SYN in all hippocampal areas, whereas lower GFAP expression was evident only in CA1 and CA3. The reduced expression of SYN in DG and CA3 was evident to male SH/CUS rats, whereas the reduced GFAP expression in CA1 and CA3 was limited to SH/CUS females. EE housing increased the hippocampal expression of both markers and protected against CUS‐associated decreases. Our findings indicate that the decreases in the expression of SYN and GFAP following CUS are region and sex‐specific and underline the neuroprotective role of EE against these CUS‐associated changes.

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Section: Discussionsupporting

confidence: 90%

Environmental enrichment initiated in adolescence restores the reduced expression of synaptophysin and GFAP in the hippocampus of chronically stressed rats in a sex‐specific manner

Dandi,

Theotokis,

Petri

et al. 2023

Developmental Psychobiology

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“…Remarkably, this phenotype was entirely reversed by an 8-week MS protocol when it was initiated in very young mice. We know that MS increases hippocampal neuroplasticity [34,35] and neurogenesis [36,37], but we did not address this process in the present study. It is also known that MS decreases neuroinflammation [38,39], and our data gives support to this important role of MS. We showed that there is a decrease in the expression of GFAP, a marker for astrocyte activation, in the HC of Adrβ3KO and WT mice after the 8-week MS protocol.…”

Section: Discussionmentioning

confidence: 84%

Multisensory Stimulation Reverses Memory Impairment in Adrβ3KO Mice

Ravache¹,

Batistuzzo²,

Nunes³

et al. 2023

Preprint

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Norepinephrine plays an important role in modulating memory consolidation and evocation through its beta-adrenergic receptors (Adrβ: β1, β2 and β3), which are expressed in the hippocampus (HC) and amygdala (AMY). Here we hypothesized that multisensory stimulation would reverse the memory impairment caused by the inactivation of the Adrβ3 with consequent inhibition of sustained glial-mediated inflammation and glutamatergic depuration. To test this, 21- and 86-day-old Adrβ3KO mice and respective controls underwent to (i) gustative and olfactive stimuli of positive and negative valence associated with (ii) intellectual challenges to reach the food in addition to (iii) objects in hidden places (iv) foraging for 8 weeks followed by (v) analysis of GFAP, Iba-1 and EAAT2 protein expression in the HC and AMY. While this protocol restored the memory impairment when applied to Adrβ3KO animals immediately after weaning, it had no effect when applied to adult animals. In fact, we observed that aging worsens the memory of Adrβ3KO mice. Although no significant expression of GFAP and Iba1 were observed in HC of young and old mice, Adrβ3KO increased EAAT2 expression HC of old mice, while MS didn’t change EAAT2 in young mice but enhanced it expression in older. Relative to AMY of old mice Adrβ3KO increased GFAP expression compared whit WT mice and MS sustained the GFAP expression and increased the EAAT2 expression compared with WT group. These results suggest that a richer and more diverse environment helps to correct memory impairment when applied right after weaning Adrβ3KO animals, also show that the changes in GFAP, Iba1 and EAAT2 expression levels in young and old mice indicate a functional significance in the process of learning and memory and that the control of neuroinflammation in limbic areas mediates this response. They also reinforce the idea that disruption of noradrenergic signaling could be involved in the cognitive impairment observed later in life.

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“…Remarkably, this phenotype was entirely reversed by an 8-week MS protocol when it was initiated in very young mice. We know that MS increases hippocampal neuroplasticity [ 26 , 27 ] and neurogenesis [ 28 , 29 ], but we did not address this process in the present study. It is also known that MS decreases neuroinflammation [ 30 , 31 ], and our data support this important role of MS. We showed that there was a decrease in the expression of GFAP, a marker for astrocyte activation, in the HC of Adrβ 3 KO and WT mice after the 8-week MS protocol.…”

Section: Discussionmentioning

confidence: 98%

Multisensory Stimulation Reverses Memory Impairment in Adrβ3KO Male Mice

Ravache

Batistuzzo

Nunes

et al. 2023

IJMS

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Norepinephrine plays an important role in modulating memory through its beta-adrenergic receptors (Adrβ: β1, β2 and β3). Here, we hypothesized that multisensory stimulation would reverse memory impairment caused by the inactivation of Adrβ3 (Adrβ3KO) with consequent inhibition of sustained glial-mediated inflammation. To test this, 21- and 86-day-old Adrβ3KO mice were exposed to an 8-week multisensory stimulation (MS) protocol that comprised gustatory and olfactory stimuli of positive and negative valence; intellectual challenges to reach food; the use of hidden objects; and the presentation of food in ways that prompted foraging, which was followed by analysis of GFAP, Iba-1 and EAAT2 protein expression in the hippocampus (HC) and amygdala (AMY). The MS protocol reduced GFAP and Iba-1 expression in the HC of young mice but not in older mice. While this protocol restored memory impairment when applied to Adrβ3KO animals immediately after weaning, it had no effect when applied to adult animals. In fact, we observed that aging worsened the memory of Adrβ3KO mice. In the AMY of Adrβ3KO older mice, we observed an increase in GFAP and EAAT2 expression when compared to wild-type (WT) mice that MS was unable to reduce. These results suggest that a richer and more diverse environment helps to correct memory impairment when applied immediately after weaning in Adrβ3KO animals and indicates that the control of neuroinflammation mediates this response.

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Environmental enrichment alters LPS-induced changes in BDNF and PSD-95 expressions during puberty

Cited by 8 publications

References 83 publications

Environmental enrichment initiated in adolescence restores the reduced expression of synaptophysin and GFAP in the hippocampus of chronically stressed rats in a sex‐specific manner

Environmental enrichment initiated in adolescence restores the reduced expression of synaptophysin and GFAP in the hippocampus of chronically stressed rats in a sex‐specific manner

Multisensory Stimulation Reverses Memory Impairment in Adrβ3KO Mice

Multisensory Stimulation Reverses Memory Impairment in Adrβ3KO Male Mice

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