Environmental hazard testing of nanobiomaterials

Amorim, Mónica J.B.; Fernández-Cruz, M. L.; Hund‐Rinke, Kerstin; Scott-Fordsmand, Janeck J.

doi:10.1186/s12302-020-00369-8

Cited by 16 publications

(10 citation statements)

References 54 publications

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“…An important aspect to consider is the suitability of current guidelines to assess the hazard of NPls. As discussed in Amorim et al [43] alternatives for highly durable materials, like plastics, can be studied using i) longer exposure durations and ii) after prior ageing and weathering of materials. These concerns have common aspects with the existing for PBTs (persistent, bioaccumulative and toxic) and vPvBs (very persistent and very bioaccumulative) compounds.…”

Section: Discussionmentioning

confidence: 99%

How Can Nanoplastics Affect the Survival, Reproduction, and Behaviour of the Soil Model Enchytraeus crypticus?

et al. 2020

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Nanoplastics (NPls) are ubiquitous in terrestrial environments, with numerous consequences for biodiversity and ecosystems. Research is urgently required to clarify the NPls environmental behaviour, fate and ecotoxicological effects to soil ecosystems. The aim of this research was to assess and comprehend the effects of polystyrene NPls to the terrestrial species Enchytraeus crypticus using survival, reproduction and avoidance behaviour as endpoints. A range of concentrations, 0.015 to 1500 mg NPls/kg LUFA 2.2 (Landwirtschaftliche Untersuchungs- und Forschungsanstalt Speyer, Germany) soil, was tested. Due to the effect of tween 20 and sodium azide (NaN3) on the NPls dispersion, the effects of these compounds were also assessed separately. After 21 d, 1200 and 1500 mg/kg NPls dispersion had significant effects on the organism survival and/or reproduction. However, these effects may be mainly associated with tween 20 and NaN3 present in the NPls dispersion and not with NPls themselves. After 48 h, there was a tendency of the organisms to avoid the NPls spiked soils, being this response significant at 0.015 mg/kg although a reduced avoidance behaviour was observed as NPls concentration increased. The present study provides screening data on the effects of NPls, alone and considering the presence of other compounds like the solvents, which is essential for regulators and strategic management of plastic pollution.

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Section: Discussionmentioning

confidence: 99%

How Can Nanoplastics Affect the Survival, Reproduction, and Behaviour of the Soil Model Enchytraeus crypticus?

et al. 2020

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“…The use of several soil organisms is recommended for ecotoxicological models for the risk assessment of chemicals. While adaptations have been proposed for engineered nanomaterials, including some specific applications such as nanobiomaterials [ 102 , 103 , 104 , 105 ], there is no specific standardized methodology to study the toxicity of nanopesticides. The model organisms currently employed in the risk assessment of chemicals include annelids (e.g., Enchytraeus crypticus [ 106 ] and Eisenia fetida [ 107 ]), nematodes (e.g., Caenorhabditis elegans [ 98 ]), and arthropods (e.g., Acanthoscelides obtectus [ 70 ], Folsomia candida [ 108 ], and Hypoaspis aculeifer ).…”

Section: Discussionmentioning

confidence: 99%

Hazard Assessment of the Effects of Acute and Chronic Exposure to Permethrin, Copper Hydroxide, Acephate, and Validamycin Nanopesticides on the Physiology of Drosophila: Novel Insights into the Cellular Internalization and Biological Effects

Demir

Kansız

Doğan

et al. 2022

IJMS

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New insights into the interactions between nanopesticides and edible plants are required in order to elucidate their impacts on human health and agriculture. Nanopesticides include formulations consisting of organic/inorganic nanoparticles. Drosophila melanogaster has become a powerful model in genetic research thanks to its genetic similarity to mammals. This project mainly aimed to generate new evidence for the toxic/genotoxic properties of different nanopesticides (a nanoemulsion (permethrin nanopesticides, 20 ± 5 nm), an inorganic nanoparticle as an active ingredient (copper(II) hydroxide [Cu(OH)2] nanopesticides, 15 ± 6 nm), a polymer-based nanopesticide (acephate nanopesticides, 55 ± 25 nm), and an inorganic nanoparticle associated with an organic active ingredient (validamycin nanopesticides, 1177 ± 220 nm)) and their microparticulate forms (i.e., permethrin, copper(II) sulfate pentahydrate (CuSO4·5H2O), acephate, and validamycin) widely used against agricultural pests, while also showing the merits of using Drosophila—a non-target in vivo eukaryotic model organism—in nanogenotoxicology studies. Significant biological effects were noted at the highest doses of permethrin (0.06 and 0.1 mM), permethrin nanopesticides (1 and 2.5 mM), CuSO4·5H2O (1 and 5 mM), acephate and acephate nanopesticides (1 and 5 mM, respectively), and validamycin and validamycin nanopesticides (1 and 2.5 mM, respectively). The results demonstrating the toxic/genotoxic potential of these nanopesticides through their impact on cellular internalization and gene expression represent significant contributions to future nanogenotoxicology studies.

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“…Although, for FOS, PECs did not cause toxic effects to the tested organisms, for other pesticides, the effects may be detected at environmentally relevant concentrations. Another important aspect is that the toxicity tests should consider longer exposure periods than the ones recommended by the guidelines [59]. In the environment, pesticides (and their metabolites and transformation products) may persist for long periods of time and the organisms may be continuously exposed [57].…”

Section: Integration Of Knowledge For Risk Assessmentmentioning

confidence: 99%

Is the Synthetic Fungicide Fosetyl-Al Safe for the Ecotoxicological Models Danio rerio and Enchytraeus crypticus?

et al. 2021

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Worldwide, pesticides have contaminated the environment, affecting non-target species. The aim of this work was to evaluate the effects of fosetyl-Al (FOS) on model organisms. Based on the 3 Rs for animal research and described guidelines, the OECD 236 and 220 were applied with some modifications. The FOS test concentrations were 0.02–0.2–2–20–200 mg/L for Danio rerio and 250–500–750–1000–1250 mg/kg for Enchytraeus crypticus. Besides the standard endpoints, additional endpoints were evaluated (D. rerio: behavior and biochemical responses; E. crypticus: extension of exposure duration (28 d (days) + 28 d) and organisms’ sizes). For D. rerio, after 96 h (h), hatching was inhibited (200 mg/L), proteins’ content increased (2 and 20 mg/L), lipids’ content decreased (2 mg/L), glutathione S-transferase activity increased (2 mg/L), and, after 120 h, larvae distance swam increased (20 mg/L). For E. crypticus, after 28 d, almost all the tested concentrations enlarged the organisms’ sizes and, after 56 d, 1250 mg/kg decreased the reproduction. In general, alterations in the organisms’ biochemical responses, behavior, and growth occurred at lower concentrations than the effects observed at the standard endpoints. This ecotoxicological assessment showed that FOS may not be considered safe for the tested species, only at higher concentrations than the predicted environmental concentrations (PECs). This research highlighted the importance of a multi-endpoint approach to assess the (eco)toxic effects of the contaminants.

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Environmental hazard testing of nanobiomaterials

Cited by 16 publications

References 54 publications

How Can Nanoplastics Affect the Survival, Reproduction, and Behaviour of the Soil Model Enchytraeus crypticus?

How Can Nanoplastics Affect the Survival, Reproduction, and Behaviour of the Soil Model Enchytraeus crypticus?

Hazard Assessment of the Effects of Acute and Chronic Exposure to Permethrin, Copper Hydroxide, Acephate, and Validamycin Nanopesticides on the Physiology of Drosophila: Novel Insights into the Cellular Internalization and Biological Effects

Is the Synthetic Fungicide Fosetyl-Al Safe for the Ecotoxicological Models Danio rerio and Enchytraeus crypticus?

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