Environmental influences on the production of pre-implantation embryos

Diercks, Ann-Kathrin; Schwab, Anna; Rittgen, Werner; Kruspel, Andreas; Heuss, Edgar; Schenkel, Johannes

doi:10.1016/j.theriogenology.2009.12.003

Cited by 12 publications

(11 citation statements)

References 10 publications

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“…As described earlier, humidity, noise, or simply the weather or the season plays an enormous role on the embryo yield [2,3]. Facilities housing laboratory animals must be run under standardized conditions, also with respect to other experiments, technical limitations, working conditions for the staff, or economic reasons.…”

Section: Discussionmentioning

confidence: 99%

“…Genetically modified mouse lines originally received from different sources were bred and expanded in-house, whereas wild-type (WT) mice with corresponding genetic backgrounds (C57BL/6; NMRI) were received from Charles River (Sulzfeld, Germany). Individually ventilated caging systems and barrier facilities (with open caging systems) were used as described in detail previously [2,3]. In the temperature experiment, ventilated cabinets with a controlled housing-temperature were used.…”

Section: Animal Experimentationmentioning

confidence: 99%

“…We have already published several studies on those factors, such as the role of the genetic background, the age of males, or the facility [1]; of pheromones, mating frequency, hygiene conditions, or annual rhythms [2]; or of environmental factors such as humidity or noise [3]. To better understand other embryo yield-influencing parameters, diet compounds might be of interest.…”

Section: Introductionmentioning

confidence: 99%

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The role of diet and housing-temperature in the production of genetically modified mouse embryos and their developmental capacity after cryopreservation

2015

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Section: Discussionmentioning

confidence: 99%

Section: Animal Experimentationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of diet and housing-temperature in the production of genetically modified mouse embryos and their developmental capacity after cryopreservation

2015

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“…Individually ventilated caging systems (IVC) and specified pathogen-free (SPF) facilities (barrier with open caging systems) were used as previously described in detail [5]. Ages of the male mice used ranged between 3 and 9 months.…”

Section: Methodsmentioning

confidence: 99%

“…State-of-the-art assessment of cryopreserved spermatozoa from small laboratory rodents involves IVF followed by embryo transfer [17,23,26]. Both of these procedures are sensitive and can be negatively influenced by the environment [5,14,15]. In addition, they require extensive resources, especially for assessment purposes.…”

Section: Introductionmentioning

confidence: 99%

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

et al. 2012

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Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or to store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. The quality of different donors within the same mouse line might be heterogeneous and the cryopreservation procedure might also be error-prone. However, only limited amounts of material are available for analysis. To improve the monitoring of frozen/thawed spermatozoa, commonly used in vitro fertilization (IVF) followed by embryo transfer were replaced with animal-free techniques. Major factors for assessing spermatozoa quality (i.e., density, viability, motility, and morphology) were evaluated by fluorescence microscopy. For this, a live/dead cell staining protocol requiring only small amounts of material was created. Membrane integrity was then examined as major parameter closely correlated with successful IVF. These complex analyses allow us to monitor frozen/thawed spermatozoa from GM mice using a relatively simple staining procedure. This approach leads to a reduction of animal experiments and contributes to the 3R principles (replacement, reduction and refinement of animal experiments).

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A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse

2020

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Environmental influences on the production of pre-implantation embryos

Cited by 12 publications

References 10 publications

The role of diet and housing-temperature in the production of genetically modified mouse embryos and their developmental capacity after cryopreservation

The role of diet and housing-temperature in the production of genetically modified mouse embryos and their developmental capacity after cryopreservation

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

A framework for high-resolution phenotyping of candidate male infertility mutants: from human to mouse

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