The role of diet and housing-temperature in the production of genetically modified mouse embryos and their developmental capacity after cryopreservation

Ramin, Michael; Denk, Nora; Schenkel, Johannes

doi:10.1016/j.theriogenology.2015.07.010

Cited by 5 publications

(5 citation statements)

References 25 publications

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“…Mating success, embryo and sperm yield, and embryo and sperm quality all remained very high regardless of whether the mice were fed diets with high or low phytoestrogen content. This disagrees with a previously published report by Ramin et al., 25 who found that the embryo yield was reduced when mice were fed a diet containing approximately 110 mg/kg genistein. Their phytoestrogen-rich diet was similar to the standard phytoestrogen diet (ca.…”

Section: Discussioncontrasting

confidence: 99%

Phytoestrogen-containing diets offer benefits for mouse embryology but lead to fewer offspring being produced

2020

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One of the most commonly used protein sources in rodent diets is soy, which is naturally rich in phytoestrogens. Although phytoestrogens have shown potential health benefits in humans, they may also have the ability to disrupt reproduction. Consequently, there has been a tendency to try to exclude them from rodent diets. In the current study, we investigated whether phytoestrogen content in the mouse diet could affect reproduction in mice used as embryo donors. Donor mice (C57BL/6JOlaHsd) were maintained with three different diets: high phytoestrogen (ca. 400 mg/kg genistein), low phytoestrogen (ca. 10 mg/kg genistein) and standard breeding diet (ca. 120 mg/kg genistein). Mice fed a high phytoestrogen diet had a high yield of plugs, embryos, and injectable embryos, as well as producing good quality embryos. Results from donor mice fed a low phytoestrogen diet were consistently but only slightly inferior, whereas mice fed a standard diet performed the poorest. Interestingly, the largest number of born and weaned offspring were observed when recipient females received embryos from the standard diet group. Sperm yield and quality of stud males did not differ between the groups. We surmize that for experimental endpoints requiring fertilized embryos it may be more beneficial to feed mice a diet containing phytoestrogen, but if the goal is to produce transgenic mice, a diet high in phytoestrogen may be inadvisable. In conclusion, care should be taken when selecting a diet for experimental mouse colonies as phytoestrogen could influence the study outcome.

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Section: Discussioncontrasting

confidence: 99%

Phytoestrogen-containing diets offer benefits for mouse embryology but lead to fewer offspring being produced

2020

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“…In our study, the best reproductive outcome was found when females were kept at 25 °C, though there was little difference between 20 °C and 25 °C. This is in accordance with studies investigating the effects of ambient temperature on germ cell and embryo quality [ 38 , 55 ]. Sperm yield in males has been shown to decrease with rising ambient temperature but female oocyte quality was constant up to 28 °C [ 38 ].…”

Section: Discussionsupporting

confidence: 92%

“…Sperm yield in males has been shown to decrease with rising ambient temperature but female oocyte quality was constant up to 28 °C [ 38 ]. In addition, embryo production following superovulation was best at temperatures of 24 °C and 26 °C [ 55 ]. Here, we were not interested in the effects of ambient temperature on gamete quality, but rather on breeding outcome, therefore all females were mated at the same temperature of 20 °C.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Ambient Temperatures on Reproductive Outcome and Stress Level of Lactating Females in Two Mouse Strains

Kolbe

Lassnig

Poelzl

et al. 2022

Animals

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Ambient temperature is an important non-biotic environmental factor influencing immunological and oncological parameters in laboratory mice. It is under discussion which temperature is more appropriate and whether the commonly used room temperature in rodent facilities of about 21 °C represents a chronic cold stress or the 30 °C of the thermoneutral zone constitutes heat stress for the animals. In this study, we selected the physiological challenging period of lactation to investigate the influence of a cage temperature of 20 °C, 25 °C, and 30 °C, respectively, on reproductive performance and stress hormone levels in two frequently used mouse strains. We found that B6D2F1 hybrid mothers weaned more pups compared to C57BL/6N mothers, and that the number of weaned pups was reduced when mothers of both strains were kept at 30 °C. Furthermore, at 30 °C, mothers and pups showed reduced body weight at weaning and offspring had longer tails. Despite pronounced temperature effects on reproductive parameters, we did not find any temperature effects on adrenocortical activity in breeding and control mice. Independent of the ambient temperature, however, we found that females raising pups showed elevated levels of faecal corticosterone metabolites (FCMs) compared to controls. Peak levels of stress hormone metabolites were measured around birth and during the third week of lactation. Our results provide no evidence of an advantage for keeping lactating mice in ambient temperatures near the thermoneutral zone. In contrast, we found that a 30 °C cage temperature during lactation reduced body mass in females and their offspring and declined female reproductive performance.

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“…GM mice were bred in house, whereas wild type (WT) mice on a common genetic background (BALB/c, C57BL/6N, DBA/2) were received from commercial breeders in an age of 21 to 23 days (Charles River Germany, Sulzfeld Germany). All animals were fed with phytoestrogen-poor diet [16] ad libitum. The age of the males ranged between three and ten months, the genetic background was in the majority C57BL/6 with an unknown number of backcross generations, males on other genetic backgrounds (BALB/c, DBA/2, BDF-hybrids) were also investigated.…”

Section: Animals and Housingmentioning

confidence: 99%

“…Some investigations with oversized, but noninfected uteri showed a dramatic drop of oocyte yields (data not shown in detail). The reasons of this hyperplasia remains unclear, it might depend on environmental factors as the diet used [16].…”

Section: Figurementioning

confidence: 99%

Age-related Yields of Mouse Sperm and Oocytes

Ramin¹,

Letz²,

Schwab³

et al. 2017

J Anim Res Nutr

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Title: Genetically modified animals, in particular mice, are unique mutants with a major scientific potential. Cryopreservation of pre-implantation embryos or of spermatozoa is a common approach to protect those lines against loss or to archive them. Prerequisite to take a mutant line out of a breeding nucleus is the cryopreservation of a sufficient number of samples and a powerful quality assessment.Background: Several common protocols to cryopreserve spermatozoa and subsequently to forward these to an in vitro-fertilization under standardized conditions are published using donors of a strictly limited age-span. In practice, several limitations happen frequently for those approaches, e.g. the availability of genetically modified males, in particular if a line is in an experimental use or the mutation leads to restrictions. Female donors are often received from external sources and are subsequently not available before weaning. They are subject of several delays to induce an early superovulation. The aim of this report is to study the suitable age span of sperm and oocyte donors.

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The role of diet and housing-temperature in the production of genetically modified mouse embryos and their developmental capacity after cryopreservation

Cited by 5 publications

References 25 publications

Phytoestrogen-containing diets offer benefits for mouse embryology but lead to fewer offspring being produced

Phytoestrogen-containing diets offer benefits for mouse embryology but lead to fewer offspring being produced

Effect of Different Ambient Temperatures on Reproductive Outcome and Stress Level of Lactating Females in Two Mouse Strains

Age-related Yields of Mouse Sperm and Oocytes

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