Environmental, Microbiological, and Immunological Features of Bacterial Biofilms Associated with Implanted Medical Devices

Caldara, Marina; Belgiovine, Cristina; Secchi, Eleonora; Rusconi, Roberto

doi:10.1128/cmr.00221-20

Cited by 67 publications

(33 citation statements)

References 427 publications

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“…The extracellular matrix confers stability to biofilms (1,2) and protects the bacterial community against chemical and mechanical insults (3,4). This protected environment makes biofilm bacteria a major cause of chronic infections in clinical environments and of clogging in industrial flow systems (5)(6)(7)(8). The diverse biopolymers composing the extracellular matrix form the three-dimensional scaffold of the biofilm and are responsible for both adhesion to the surface and cohesion within the biofilm.…”

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confidence: 99%

The structural role of bacterial eDNA in the formation of biofilm streamers

Secchi

Savorana

Vitale

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Significance Streamers, filamentous bacterial biofilms formed in flowing systems, are ubiquitous in natural and artificial environments, where they cause clogging of devices and spreading of infections. Despite their impact, little is known about the nature and properties of streamers and their response to fluid flow. Here, we uncover the specific contribution of bacterial secreted extracellular DNA and exopolysaccharide Pel, two important components in Pseudomonas aeruginosa biofilms, to the formation and the mechanical properties of the streamers. We then show how this knowledge can be used to control biofilm streamer formation, both to inhibit or to promote it.

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confidence: 99%

The structural role of bacterial eDNA in the formation of biofilm streamers

Secchi

Savorana

Vitale

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…These infections anchored to the surface are known as biofilms. Biofilms are formed by complex groups of microbial cells of both Gram-positive and Gram-negative bacteria, generating a protective extracellular polysaccharide matrix [7]. Removal of these communities is challenging since biofilms are resilient to the host's immune system and antibiotic treatment [8].…”

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confidence: 99%

Implant Surfaces Containing Bioglasses and Ciprofloxacin as Platforms for Bone Repair and Improved Resistance to Microbial Colonization

et al. 2022

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Coatings are an attractive and challenging selection for improving the bioperformance of metallic devices. Composite materials based on bioglass/antibiotic/polymer are herein proposed as multifunctional thin films for hard tissue implants. We deposited a thin layer of the polymeric material by matrix-assisted pulsed laser evaporation—MAPLE onto Ti substrates. A second layer consisting of bioglass+antibiotic was applied by MAPLE onto the initial thin film. The antimicrobial activity of MAPLE-deposited thin films was evaluated on Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa standard strains. The biocompatibility of obtained thin films was assessed on mouse osteoblast-like cells. The results of our study revealed that the laser-deposited coatings are biocompatible and resistant to microbial colonization and biofilm formation. Accordingly, they can be considered viable candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission.

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“…Biofilms are undesired in a wide range of industrial and medical settings, causing problems ranging from industrial biofouling to chronic infections of medical implants [13][14][15][16]. In most cases, the biofilm forms on a solid substrate and is exposed to fluid flow, which influences the transport of nutrients, signal molecules and metabolites, as well as the structure of biofilms through drag stresses [17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Experimental challenges in determining the rheological properties of bacterial biofilms

2022

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Bacterial biofilms are communities living in a matrix consisting of self-produced, hydrated extracellular polymeric substances. Most microorganisms adopt the biofilm lifestyle since it protects by conferring resistance to antibiotics and physico-chemical stress factors. Consequently, mechanical removal is often necessary but rendered difficult by the biofilm’s complex, viscoelastic response, and adhesive properties. Overall, the mechanical behaviour of biofilms also plays a role in the spreading, dispersal and subsequent colonization of new surfaces. Therefore, the characterization of the mechanical properties of biofilms plays a crucial role in controlling and combating biofilms in industrial and medical environments. We performed in situ shear rheological measurements of Bacillus subtilis biofilms grown between the plates of a rotational rheometer under well-controlled conditions relevant to many biofilm habitats. We investigated how the mechanical history preceding rheological measurements influenced biofilm mechanics and compared these results to the techniques commonly used in the literature. We also compare our results to measurements using interfacial rheology on bacterial pellicles formed at the air–water interface. This work aims to help understand how different growth and measurement conditions contribute to the large variability of mechanical properties reported in the literature and provide a new tool for the rigorous characterization of matrix components and biofilms.

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Environmental, Microbiological, and Immunological Features of Bacterial Biofilms Associated with Implanted Medical Devices

Cited by 67 publications

References 427 publications

The structural role of bacterial eDNA in the formation of biofilm streamers

The structural role of bacterial eDNA in the formation of biofilm streamers

Implant Surfaces Containing Bioglasses and Ciprofloxacin as Platforms for Bone Repair and Improved Resistance to Microbial Colonization

Experimental challenges in determining the rheological properties of bacterial biofilms

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